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Comparative analysis of SERPINA1 gene expression in tumor cell lines

Comparative analysis of SERPINA1 gene expression in tumor cell lines The expression of SERPINA1 gene in prostate (DU145, PC-3 and LNCaP) and liver (HepG2) tumor cell lines was studied. Alpha1-antitrypsin (AAT) level in the whole cell extracts, secretomes, subcellular fractions and SERPINA1 mRNA level in the corresponding cells were detected. Discordance between expression at these two levels in PC-3 and LNCaP lines was revealed. A new 37 kDa AAT N-terminus truncated isoform was detected in the nuclear extracts of some prostate tumor cell lines. The mechanism of 37 kDa AAT isoform intracellular retention was proposed. Two polyadenylation sites in the 3'-untranslated region of SERPINA1 transcripts were identified. A SERPINA1 gene 3'-untranslated region influence on AAT translation has been discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Moscow University Biological Sciences Bulletin Springer Journals

Comparative analysis of SERPINA1 gene expression in tumor cell lines

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References (20)

Publisher
Springer Journals
Copyright
Copyright © 2015 by Allerton Press, Inc.
Subject
Life Sciences; Biochemistry, general; Cell Biology; Life Sciences, general; Plant Sciences; Zoology
ISSN
0096-3925
eISSN
1934-791X
DOI
10.3103/S0096392515030086
Publisher site
See Article on Publisher Site

Abstract

The expression of SERPINA1 gene in prostate (DU145, PC-3 and LNCaP) and liver (HepG2) tumor cell lines was studied. Alpha1-antitrypsin (AAT) level in the whole cell extracts, secretomes, subcellular fractions and SERPINA1 mRNA level in the corresponding cells were detected. Discordance between expression at these two levels in PC-3 and LNCaP lines was revealed. A new 37 kDa AAT N-terminus truncated isoform was detected in the nuclear extracts of some prostate tumor cell lines. The mechanism of 37 kDa AAT isoform intracellular retention was proposed. Two polyadenylation sites in the 3'-untranslated region of SERPINA1 transcripts were identified. A SERPINA1 gene 3'-untranslated region influence on AAT translation has been discussed.

Journal

Moscow University Biological Sciences BulletinSpringer Journals

Published: Sep 23, 2015

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