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Coimmobilization of Superoxide Dismutase, Catalase, and Peroxidase

Coimmobilization of Superoxide Dismutase, Catalase, and Peroxidase Various orders of sequential coimmobilization of superoxide dismutase (SOD), catalase, and horseradish peroxidase (HRP) were tested in order to prepare a multienzyme antioxidant complex of these enzymes. Simultaneous coimmobilization of catalase with a preliminarily cross-linked complex between SOD and HRP was found to be the optimum procedure. The catalytic enzyme activity and working stability of catalase was tested kinetically in the multienzyme complexes prepared by different methods. The effects of ascorbic acid, glutathione, and ethanol on the kinetic parameters of catalase were studied. A possible scheme of H2O2 degradation catalyzed by coimmobilized SOD, catalase, and HRP in the presence of reducing substrates is suggested. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Coimmobilization of Superoxide Dismutase, Catalase, and Peroxidase

Applied Biochemistry and Microbiology , Volume 37 (1) – Oct 19, 2004

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References (9)

Publisher
Springer Journals
Copyright
Copyright © 2001 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Medical Microbiology; Biochemistry, general; Microbiology
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1023/A:1002840324543
Publisher site
See Article on Publisher Site

Abstract

Various orders of sequential coimmobilization of superoxide dismutase (SOD), catalase, and horseradish peroxidase (HRP) were tested in order to prepare a multienzyme antioxidant complex of these enzymes. Simultaneous coimmobilization of catalase with a preliminarily cross-linked complex between SOD and HRP was found to be the optimum procedure. The catalytic enzyme activity and working stability of catalase was tested kinetically in the multienzyme complexes prepared by different methods. The effects of ascorbic acid, glutathione, and ethanol on the kinetic parameters of catalase were studied. A possible scheme of H2O2 degradation catalyzed by coimmobilized SOD, catalase, and HRP in the presence of reducing substrates is suggested.

Journal

Applied Biochemistry and MicrobiologySpringer Journals

Published: Oct 19, 2004

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