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CD4+CD25highCD127- regulatory T-cells in COPD: smoke and drugs effect

CD4+CD25highCD127- regulatory T-cells in COPD: smoke and drugs effect Background: Chronic obstructive pulmonary disease (COPD) is a progressive lung disorder characterized by poorly reversible airway obstruction and its pathogenesis remains largely misunderstood. Local changes of regulatory T-cell populations in the lungs of COPD patients have been demonstrated although data concerning their pathologic role are contrasting. The aim of our study was to evaluate the relative percentage of regulatory T-cells in the peripheral blood of current and former smoker subjects, affected or not by COPD. Furthermore, the effect of different concentrations of budesonide and formoterol, on regulatory T-cells has been investigated. + high - Methods: T regulatory lymphocytes were isolated and assessed as CD4 CD25 CD127 cells by flow cytometry and cultured for 48 hours in the absence or in the presence of budesonide and/or formoterol at different doses. + high - Results: CD4 CD25 CD127 regulatory T-cells percentage was significantly reduced in COPD patients, both + high - current and former smokers, with respect to volunteers. Furthermore, CD4 CD25 CD127 cells of COPD patients showed a not statistically significant response to drugs compared to healthy subjects. + high - Discussion: Our results evidenced a different behaviour of CD4 CD25 CD127 Treg cells in COPD patients after in vitro http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png World Allergy Organization Journal Springer Journals

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References (50)

Publisher
Springer Journals
Copyright
Copyright © 2016 by Chiappori et al.
Subject
Medicine & Public Health; Allergology; Immunology
eISSN
1939-4551
DOI
10.1186/s40413-016-0095-2
pmid
26904157
Publisher site
See Article on Publisher Site

Abstract

Background: Chronic obstructive pulmonary disease (COPD) is a progressive lung disorder characterized by poorly reversible airway obstruction and its pathogenesis remains largely misunderstood. Local changes of regulatory T-cell populations in the lungs of COPD patients have been demonstrated although data concerning their pathologic role are contrasting. The aim of our study was to evaluate the relative percentage of regulatory T-cells in the peripheral blood of current and former smoker subjects, affected or not by COPD. Furthermore, the effect of different concentrations of budesonide and formoterol, on regulatory T-cells has been investigated. + high - Methods: T regulatory lymphocytes were isolated and assessed as CD4 CD25 CD127 cells by flow cytometry and cultured for 48 hours in the absence or in the presence of budesonide and/or formoterol at different doses. + high - Results: CD4 CD25 CD127 regulatory T-cells percentage was significantly reduced in COPD patients, both + high - current and former smokers, with respect to volunteers. Furthermore, CD4 CD25 CD127 cells of COPD patients showed a not statistically significant response to drugs compared to healthy subjects. + high - Discussion: Our results evidenced a different behaviour of CD4 CD25 CD127 Treg cells in COPD patients after in vitro

Journal

World Allergy Organization JournalSpringer Journals

Published: Feb 12, 2016

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