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Gong Cheng Xue Bao (Chinense
ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 4, pp. 323–343. © Pleiades Publishing, Inc., 2019. Russian Text © The Author(s), 2019, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2019, Vol. 55, No. 4, pp. 315–337. Biotechnological Aspects of the Enzymatic Preparation of Bioactive Chitooligosaccharides (Review) a, a b G. E. Aktuganov *, A. I. Melentiev , and V. P. Varlamov Ufa Institute of Biology, Ufa Federal Research Center, Russian Academy of Sciences, Ufa, 450054 Russia Institute of Bioengineering, Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences, Moscow, 119071 Russia *e-mail: gleakt@anrb.ru Received October 19, 2018; revised November 14, 2018; accepted February 20, 2019 Abstract—The review describes recent technologies for the enzymatic preparation of chitooligosaccharides with chitosanases. Features of the microbial synthesis of chitosanases and approaches to the implementation of their biotechnological production are also highlighted. Tendencies in the development of methods and technologies for the targeted preparation of chitosan oligomers with the use of bioreactors and immobilized enzymes are considered. Keywords: chitosanase, chitosan, chitooligosaccharides, enzymatic depolymerization, membrane bioreac- tors, immobilized enzymes DOI: 10.1134/S00036838190 40021 INTRODUCTION the purification and drying of the target oligomeric product (Fig. 1). A serious limitation to the widespread Water-soluble oligomeric chitosan
Applied Biochemistry and Microbiology – Springer Journals
Published: Jul 8, 2019
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