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Abstract We have developed an antibody microarray chip for biomarker analysis in rat livers exposed to five different toxic pollutants: volatile organic compounds (VOCs), dichloromethane, ethylbenzene, and trichloroethylene; and polycyclic aromatic hydrocarbons (PAHs), benzoanthracene and phenanthrene. The antibody chip consisted of antibodies against different biomarkers: alpha-2μ globulin, glutathione S transferase (GST)-α, Hemoglobin β, and Phenylalanine hydroxylase. VOC- and PAH-treated rat livers showed differential expression patterns of the biomarker proteins. These results demonstrate that a protein chipbased antibody array is useful for the analysis of biomarkers induced by environmental toxic pollutants and can be a powerful tool to study the mechanisms of pollutants in biological samples.
BioChip Journal – Springer Journals
Published: Sep 1, 2010
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