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Bioluminescent Toxicity Assay of Polyethylenimine-Based Sorbents

Bioluminescent Toxicity Assay of Polyethylenimine-Based Sorbents The toxicity of polyethylenimine-based sorbents and their extracts was evaluated, and their effect on the bioluminescence of Photobacterium phosphoreum photobacteria was studied. These test bacteria are commonly used as objects to evaluate the toxicity of various materials. The analyzed materials were synthesized via polyethylenimine (PEI) cross-linking with diethylene glycol diglycidyl ether (DGDE) at mass contents of the latter of 1.9–120.0% with subsequent freezing. It was found that the degree of luminescence inhibition in P. phosphoreum cells depended on the PEI/DGDE ratio in the sorbent. Sorbents with a high DGDE content (60–120%) did not affect the cell luminescence activity, while those with a lower percentage of the cross-linker (0.9–30%) exhibited a pronounced inhibitory effect on luminescence of photobacteria according to the data obtained with the standard biotesting method. It was also established that the inhibitory effect of sorbents with a reduced DGDE percentage (<30%) in a phosphate buffer was significantly lower than that in salt solutions. Water and ethanol extracts of sorbents with a DGDE mass proportion of more than 15% did not significantly inhibit the luminescence of P. phosphoreum in 1 h of incubation. The immobilization of P. phosphoreum cells on the surface and internal parts of the studied sorbents was observed via scanning electron microscopy. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

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Publisher
Springer Journals
Copyright
Copyright © Pleiades Publishing, Inc. 2021. ISSN 0003-6838, Applied Biochemistry and Microbiology, 2021, Vol. 57, No. 7, pp. 828–835. © Pleiades Publishing, Inc., 2021. Russian Text © The Author(s), 2020, published in Biotekhnologiya, 2020, Vol. 36, No. 3, pp. 73–81.
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/s0003683821070061
Publisher site
See Article on Publisher Site

Abstract

The toxicity of polyethylenimine-based sorbents and their extracts was evaluated, and their effect on the bioluminescence of Photobacterium phosphoreum photobacteria was studied. These test bacteria are commonly used as objects to evaluate the toxicity of various materials. The analyzed materials were synthesized via polyethylenimine (PEI) cross-linking with diethylene glycol diglycidyl ether (DGDE) at mass contents of the latter of 1.9–120.0% with subsequent freezing. It was found that the degree of luminescence inhibition in P. phosphoreum cells depended on the PEI/DGDE ratio in the sorbent. Sorbents with a high DGDE content (60–120%) did not affect the cell luminescence activity, while those with a lower percentage of the cross-linker (0.9–30%) exhibited a pronounced inhibitory effect on luminescence of photobacteria according to the data obtained with the standard biotesting method. It was also established that the inhibitory effect of sorbents with a reduced DGDE percentage (<30%) in a phosphate buffer was significantly lower than that in salt solutions. Water and ethanol extracts of sorbents with a DGDE mass proportion of more than 15% did not significantly inhibit the luminescence of P. phosphoreum in 1 h of incubation. The immobilization of P. phosphoreum cells on the surface and internal parts of the studied sorbents was observed via scanning electron microscopy.

Journal

Applied Biochemistry and MicrobiologySpringer Journals

Published: Dec 1, 2021

Keywords: bioluminescence; biotest; toxicity analysis; photobacteria; polymer sorbents; polyethylenimine

References