Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

BG-58S macrospheres produced using BG powder synthesized by alkali-mediated sol–gel process and different phosphorus precursor

BG-58S macrospheres produced using BG powder synthesized by alkali-mediated sol–gel process and... PurposeBioactive glass (BG) spheres with uniform shape and specific size variation are allowed to be packed into a 3D arrangement, which results in an open porosity that improves bone growth.MethodsBG-58S macrospheres were produced using BG powder synthesized by alkali-mediated sol–gel process and different phosphorus precursors (TEP or phosphoric acid-AF). Macrospheres (MAF-1 M, MAF-2 M, MTEP-1 M, and MTEP-2 M) were characterized as to surface morphology and size, theoretical density, and specific surface area/pore size distribution. In vitro bioactivity was evaluated in simulated body fluid (SBF). In vitro tests were conducted (for MAF-2 M and MTEP-2 M) as cell viability, total protein content, determination of alkaline phosphatase, cell adhesion by means of SEM, and mineralization nodules formation compared to commercial product (BG-45S5). Biological performance was verified through histological and histomorphometric analyses around the samples: BG-45S5, MTEP-2 M, and control (clot).ResultsAll physicochemical characterizations demonstrated favorable macrospheres for application in bone grafting. MTEP-2 M and MAF-2 M showed higher cell viability and total protein content when compared to BG-45S5 with a statistical difference (p < 0.05); however, no statistical difference was detected among the groups regarding the determination of alkaline phosphatase (p > 0.05). Cells adhered to the surface were observed for all samples as well as nodules of mineralization. The results referring to in vivo biological assays showed no statistical difference between the experimental groups MTEP-2 M, BV45S5, and clot control in the two periods evaluated (p > 0.05).ConclusionThe method used in this study was able to prepare macrosphere bioglass, and this material seems to be a promising biomaterial to improve bone tissue regeneration. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Research on Biomedical Engineering Springer Journals

BG-58S macrospheres produced using BG powder synthesized by alkali-mediated sol–gel process and different phosphorus precursor

Download PDF
18 pages

Loading next page...
 
/lp/springer-journals/bg-58s-macrospheres-produced-using-bg-powder-synthesized-by-alkali-TLfmEzVqpG

References (55)

Publisher
Springer Journals
Copyright
Copyright © The Author(s), under exclusive licence to The Brazilian Society of Biomedical Engineering 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
ISSN
2446-4732
eISSN
2446-4740
DOI
10.1007/s42600-023-00312-9
Publisher site
See Article on Publisher Site

Abstract

PurposeBioactive glass (BG) spheres with uniform shape and specific size variation are allowed to be packed into a 3D arrangement, which results in an open porosity that improves bone growth.MethodsBG-58S macrospheres were produced using BG powder synthesized by alkali-mediated sol–gel process and different phosphorus precursors (TEP or phosphoric acid-AF). Macrospheres (MAF-1 M, MAF-2 M, MTEP-1 M, and MTEP-2 M) were characterized as to surface morphology and size, theoretical density, and specific surface area/pore size distribution. In vitro bioactivity was evaluated in simulated body fluid (SBF). In vitro tests were conducted (for MAF-2 M and MTEP-2 M) as cell viability, total protein content, determination of alkaline phosphatase, cell adhesion by means of SEM, and mineralization nodules formation compared to commercial product (BG-45S5). Biological performance was verified through histological and histomorphometric analyses around the samples: BG-45S5, MTEP-2 M, and control (clot).ResultsAll physicochemical characterizations demonstrated favorable macrospheres for application in bone grafting. MTEP-2 M and MAF-2 M showed higher cell viability and total protein content when compared to BG-45S5 with a statistical difference (p < 0.05); however, no statistical difference was detected among the groups regarding the determination of alkaline phosphatase (p > 0.05). Cells adhered to the surface were observed for all samples as well as nodules of mineralization. The results referring to in vivo biological assays showed no statistical difference between the experimental groups MTEP-2 M, BV45S5, and clot control in the two periods evaluated (p > 0.05).ConclusionThe method used in this study was able to prepare macrosphere bioglass, and this material seems to be a promising biomaterial to improve bone tissue regeneration.

Journal

Research on Biomedical Engineering – Springer Journals

Published: Sep 15, 2023

Keywords: BG-58S macrospheres; Phosphorus precursor; Alkali-mediated sol–gel; Osteoblast; Bone repair

There are no references for this article.