Access the full text.
Sign up today, get DeepDyve free for 14 days.
A. Saovapakhiran, A. D'emanuele, D. Attwood, J. Penny (2009)
Surface modification of PAMAM dendrimers modulates the mechanism of cellular internalization.Bioconjugate chemistry, 20 4
Chaojie Wang, J. Delcros, J. Biggerstaff, O. Phanstiel (2003)
Molecular requirements for targeting the polyamine transport system. Synthesis and biological evaluation of polyamine-anthracene conjugates.Journal of medicinal chemistry, 46 13
J. Choi, E. Lee, H. Jang, J. Park (2001)
New cationic liposomes for gene transfer into mammalian cells with high efficiency and low toxicity.Bioconjugate chemistry, 12 1
Y. Takechi, Hirokazu Tanaka, Hiroki Kitayama, Haruka Yoshii, Masafumi Tanaka, H. Saito (2012)
Comparative study on the interaction of cell-penetrating polycationic polymers with lipid membranes.Chemistry and physics of lipids, 165 1
C. Dufès, I. Uchegbu, A. Schätzlein (2005)
Dendrimers in gene delivery.Advanced drug delivery reviews, 57 15
D. Luo, W. Saltzman (2000)
Enhancement of transfection by physical concentration of DNA at the cell surfaceNature Biotechnology, 18
J. Haensler, F. Szoka (1993)
Polyamidoamine cascade polymers mediate efficient transfection of cells in culture.Bioconjugate chemistry, 4 5
J. Rothbard, T. Jessop, R. Lewis, B. Murray, P. Wender (2004)
Role of membrane potential and hydrogen bonding in the mechanism of translocation of guanidinium-rich peptides into cells.Journal of the American Chemical Society, 126 31
D. Fischer, Youxin Li, B. Ahlemeyer, J. Krieglstein, T. Kissel (2003)
In vitro cytotoxicity testing of polycations: influence of polymer structure on cell viability and hemolysis.Biomaterials, 24 7
Tae-il Kim, Jung-un Baek, Cheng Bai, Jong-sang Park (2007)
Arginine-conjugated polypropylenimine dendrimer as a non-toxic and efficient gene delivery carrier.Biomaterials, 28 11
Tae-il Kim, M. Ou, Minhyung Lee, S. Kim (2009)
Arginine-grafted bioreducible poly(disulfide amine) for gene delivery systems.Biomaterials, 30 4
H. Åmand, Hanna Rydberg, L. Fornander, P. Lincoln, B. Nordén, E. Esbjörner (2012)
Cell surface binding and uptake of arginine- and lysine-rich penetratin peptides in absence and presence of proteoglycans.Biochimica et biophysica acta, 1818 11
Ajay Kumar, V. Yellepeddi, G. Davies, K. Strychar, S. Palakurthi (2010)
Enhanced gene transfection efficiency by polyamidoamine (PAMAM) dendrimers modified with ornithine residues.International journal of pharmaceutics, 392 1-2
H. Åmand, Kristina Fant, B. Nordén, E. Esbjörner (2008)
Stimulated endocytosis in penetratin uptake: effect of arginine and lysine.Biochemical and biophysical research communications, 371 4
J. Choi, K. Nam, Jong-yeun Park, Jung‐Bin Kim, Ja-Kyeong Lee, J. Park (2004)
Enhanced transfection efficiency of PAMAM dendrimer by surface modification with L-arginine.Journal of controlled release : official journal of the Controlled Release Society, 99 3
L. Vincent, J. Varet, J. Pille, H. Bompais, P. Opolon, A. Maksimenko, C. Malvy, M. Mirshahi, He Lu, J. Vannier, C. Soria, Hong Li (2003)
Efficacy of dendrimer‐mediated angiostatin and TIMP‐2 gene delivery on inhibition of tumor growth and angiogenesis: In vitro and in vivo studiesInternational Journal of Cancer, 105
M. Manunta, P. Tan, P. Sagoo, K. Kashefi, A. George (2004)
Gene delivery by dendrimers operates via a cholesterol dependent pathway.Nucleic acids research, 32 9
V D Gordon A Mishra (2008)
10.1002/ange.200704444Angew. Chem. Int. Ed. Engl., 120
R Edrada-Ebel H Aldawsari (2011)
10.1016/j.biomaterials.2011.04.079Biomaterials, 32
Hibah Aldawsari, Behin Raj, R. Edrada-Ebel, D. Blatchford, R. Tate, L. Tetley, C. Dufès (2011)
Enhanced gene expression in tumors after intravenous administration of arginine-, lysine- and leucine-bearing polyethylenimine polyplex.Nanomedicine : nanotechnology, biology, and medicine, 7 5
G. Yu, Yun Bae, Hye Choi, Bokyung Kong, I. Choi, J. Choi (2011)
Synthesis of PAMAM dendrimer derivatives with enhanced buffering capacity and remarkable gene transfection efficiency.Bioconjugate chemistry, 22 6
Yu Gao, Zhenghong Xu, Shang-wei Chen, Wangwen Gu, Lingli Chen, Yaping Li (2008)
Arginine-chitosan/DNA self-assemble nanoparticles for gene delivery: In vitro characteristics and transfection efficiency.International journal of pharmaceutics, 359 1-2
J. Kukowska-Latallo, A. Bielinska, Jennifer Johnson, Ralph Spindler, D. Tomalia, James Baker (1996)
Efficient transfer of genetic material into mammalian cells using Starburst polyamidoamine dendrimers.Proceedings of the National Academy of Sciences of the United States of America, 93 10
Molly Martin, K. Rice (2007)
Peptide-guided gene deliveryThe AAPS Journal, 9
D. Fischer, T. Bieber, Youxin Li, H. Elsässer, T. Kissel (1999)
A Novel Non-Viral Vector for DNA Delivery Based on Low Molecular Weight, Branched Polyethylenimine: Effect of Molecular Weight on Transfection Efficiency and CytotoxicityPharmaceutical Research, 16
R. Mahat, O. Monera, L. Smith, A. Rolland (1999)
Peptide-based gene delivery.Current opinion in molecular therapeutics, 1 2
A. Pathak, Pradeep Kumar, K. Chuttani, Sanyog Jain, A. Mishra, S. Vyas, K. Gupta (2009)
Gene expression, biodistribution, and pharmacoscintigraphic evaluation of chondroitin sulfate-PEI nanoconstructs mediated tumor gene therapy.ACS nano, 3 6
D. Luo, W. Saltzman (2000)
Synthetic DNA delivery systemsNature Biotechnology, 18
Wei Chen, N. Turro, D. Tomalia (2000)
Using Ethidium Bromide To Probe the Interactions between DNA and DendrimersLangmuir, 16
T. Theodossiou, Alexandros Pantos, Ioannis Tsogas, C. Paleos (2008)
Guanidinylated Dendritic Molecular Transporters: Prospective Drug Delivery Systems and Application in Cell TransfectionChemMedChem, 3
N. Sakai, S. Futaki, S. Matile (2006)
Anion hopping of (and on) functional oligoarginines: from chloroform to cells.Soft matter, 2 8
Abstract In this study, we characterized the polyamidoamine (PAMAM) dendrimer derivatives conjugated with basic amino acids as effective nonviral vector systems for gene delivery. Using PAMAM dendrimer (generation 4) as a core polymer, we further synthesized PAMAM G4-Histidine-Lysine (PAMAM G4-H-K) and PAMAM G4-Histidine-Ornithine (PAMAM G4-H-O). Lysine and ornithine have cationic charged groups that can contribute to the condensation of DNA and interaction with cellular membranes. Histidine has an imidazole ring group that can induce a proton buffering effect. In this report, we performed experiment to evaluate the basic amino acid-PAMAM conjugates as efficient and safe gene carriers. The mean diameter and zeta potential value of the PAMAM conjugates/DNA complex were measured to be around 100 nm and 30 mV, respectively. It was observed that the PAMAM derivatives and plasmid DNA can form polyplexes at weight ratio 1.5 by agarose gel retardation and PicoGreen reagent assay. Furthermore, the PAMAM derivatives have shown high buffering capacity compared to the native PAMAM dendrimer. We performed the cytotoxicity and transfection assay in the HeLa, HepG2, HEK 293, and NIH3T3 cell lines. While the transfection efficiency was remarkable in all cell lines tested, the cytotoxicity level was very low. Based on these characteristics, it is suggested that the basic amino acid-conjugated PAMAM dendrimers could be utilized as promising gene delivery polymeric vectors for effective gene therapy.
"Macromolecular Research" – Springer Journals
Published: May 1, 2014
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.