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K. Kramer, M. Kanost, T. Hopkins, Haobo Jiang, Yu Zhu, R. Xu, J. Kerwin, F. Tureček (2001)
Oxidative conjugation of catechols with proteins in insect skeletal systemsTetrahedron, 57
Lee Freiburger, Oliver Baettig, T. Sprules, A. Berghuis, K. Auclair, A. Mittermaier (2011)
Competing allosteric mechanisms modulate substrate binding in a dimeric enzymeNature Structural &Molecular Biology, 18
Naixia Zhang, K. Walters (2009)
Insights into how protein dynamics affects arylamine N-acetyltransferase catalysis.Biochemical and biophysical research communications, 385 3
Jacqueline Angelis, J. Gastel, D. Klein, P. Cole (1998)
Kinetic Analysis of the Catalytic Mechanism of Serotonin N-Acetyltransferase (EC 2.3.1.87)*The Journal of Biological Chemistry, 273
F. Dai, L. Qiao, X. Tong, C. Cao, Peng Chen, Jun Chen, Cheng Lu, Z. Xiang (2010)
Mutations of an Arylalkylamine-N-acetyltransferase, Bm-iAANAT, Are Responsible for Silkworm Melanism Mutant*The Journal of Biological Chemistry, 285
M. Grunstein (1997)
Histone acetylation in chromatin structure and transcriptionNature, 389
B. Sloley (2004)
Metabolism of monoamines in invertebrates: the relative importance of monoamine oxidase in different phyla.Neurotoxicology, 25 1-2
F. Dyda, David Klein, A. Hickman (2000)
GCN5-related N-acetyltransferases: a structural overview.Annual review of biophysics and biomolecular structure, 29
P. Mehere, Q. Han, B. Christensen, Jianyong Li (2011)
Identification and characterization of two arylalkylamine N-acetyltransferases in the yellow fever mosquito, Aedes aegypti.Insect biochemistry and molecular biology, 41 9
David Klein, P. Roseboom, S. Coon (1996)
New light is shining on the melatonin rhythm enzyme The first postcloning viewTrends in Endocrinology & Metabolism, 7
F. Delaglio, S. Grzesiek, G. Vuister, G. Zhu, John Pfeifer, A. Bax (1995)
NMRPipe: A multidimensional spectral processing system based on UNIX pipesJournal of Biomolecular NMR, 6
D. Klein (2007)
Arylalkylamine N-Acetyltransferase: “the Timezyme”*Journal of Biological Chemistry, 282
J. Arendt (1995)
Significance of Melatonin in Humans
D. Klein, J. Weller (1970)
Indole Metabolism in the Pineal Gland: A Circadian Rhythm in N-AcetyltransferaseScience, 169
Naixia Zhang, L. Liu, Fen Liu, C. Wagner, P. Hanna, K. Walters (2006)
NMR-based model reveals the structural determinants of mammalian arylamine N-acetyltransferase substrate specificity.Journal of molecular biology, 363 1
Daniela Brodbeck, R. Amherd, Patrick Callaerts, Edith Hintermann, Urs Meyer, Markus Affolter (1998)
Molecular and biochemical characterization of the aaNAT1 (Dat) locus in Drosophila melanogaster: differential expression of two gene products.DNA and cell biology, 17 7
Susumu Hiragaki, Takeshi Suzuki, A. Mohamed, M. Takeda (2015)
Structures and functions of insect arylalkylamine N-acetyltransferase (iaaNAT); a key enzyme for physiological and behavioral switch in arthropodsFrontiers in Physiology, 6
Q. Han, H. Robinson, H. Ding, B. Christensen, Jianyong Li (2012)
Evolution of insect arylalkylamine N-acetyltransferases: Structural evidence from the yellow fever mosquito, Aedes aegyptiProceedings of the National Academy of Sciences, 109
D. Waugh (1996)
Genetic tools for selective labeling of proteins with alpha-15N-amino acids.Journal of biomolecular NMR, 8 2
Abu Ud-Din, A. Tikhomirova, A. Roujeinikova (2016)
Structure and Functional Diversity of GCN5-Related N-Acetyltransferases (GNAT)International Journal of Molecular Sciences, 17
M. Vetting, Luiz Carvalho, Michael Yu, S. Hegde, S. Magnet, S. Roderick, J. Blanchard (2005)
Structure and functions of the GNAT superfamily of acetyltransferases.Archives of biochemistry and biophysics, 433 1
Miquel Barberà, B. Mengual, Jorge Collantes-Alegre, T. Cortés, A. González, D. Martínez-Torres (2013)
Identification, characterization and analysis of expression of genes encoding arylalkylamine N‐acetyltransferases in the pea aphid Acyrthosiphon pisumInsect Molecular Biology, 22
M. Osanai-Futahashi, Takahiro Ohde, Junya Hirata, K. Uchino, R. Futahashi, T. Tamura, T. Niimi, H. Sezutsu (2012)
A visible dominant marker for insect transgenesisNature Communications, 3
Seulgi Mun, Mi Noh, N. Dittmer, S. Muthukrishnan, K. Kramer, M. Kanost, Y. Arakane (2015)
Cuticular protein with a low complexity sequence becomes cross-linked during insect cuticle sclerotization and is required for the adult moltScientific Reports, 5
A. Norris, E. Serpersu (2013)
Ligand promiscuity through the eyes of the aminoglycoside N3 acetyltransferase IIaProtein Science, 22
Arylalkylamine N-acetyltransferases (AANATs) catalyze the transfer of an acetyl group from the acetyl-group donor, acetyl-CoA, to an arylalkylamine acceptor. Although a single AANAT has been identified in mammals, insects utilize multiple AANATs in a diverse array of biological processes. AANATs belong to the GCN5-related acetyltransferase (GNAT) superfamily of enzymes, which despite their overall very low sequence homology, are characterized by a well conserved catalytic core domain. The structural properties of many GNATs have been extensively studied by X-ray crystallography that revealed common features during the catalytic cycle. Here we report the 1H, 13C and 15N backbone NMR resonance assignment of the 24 kDa AANAT3 from Bombyx mori (bmAANAT3) as a first step towards understanding the role of protein dynamics in the catalytic properties of AANATs. Our preliminary solution NMR studies reveal that bmAANAT3 is well-folded in solution. The P-loop, which is responsible for cofactor binding, is flexible in the free-state, while a large region of the enzyme interconverts between two distinct conformations in the slow exchange regime.
Biomolecular NMR Assignments – Springer Journals
Published: Feb 24, 2017
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