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Autoclaved mycelium induces efficiently the production of hydrolytic enzymes for protoplast preparation of autologous fungus

Autoclaved mycelium induces efficiently the production of hydrolytic enzymes for protoplast... The production of hydrolytic enzymes by the mutant Trichoderma reesei Rut-C30 when cultivated in the presence of various carbon sources: glucose, wheat bran and autoclaved mycelium of Penicillium occitanis CT1 has been studied. Glucose was shown to repress all studied hydrolases, 3% of either wheat bran or autoclaved cell walls led to high titers of enzymes, and were favorably comparable to commercial lysing enzymes (LE). The lysing enzyme cocktail obtained when T. reesei Rut-C30 was cultivated in the presence of autoclaved P. occitanis CT1 mycelia appeared to be a most effective for P. occitanis CT1 protoplast formation. Maximal yield of protoplasts reached 13 × 106 protoplasts/mL while commercial LE preparation released only 4 × 106 protoplasts/mL. The protoplast yield was affected also by the osmotic stabilizer, with KCl giving the best results. Our results suggest that to achieve the best protoplastization rate, the enzyme preparation should be obtained following induction by the autoclaved mycelium of the autologous fungus. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Autoclaved mycelium induces efficiently the production of hydrolytic enzymes for protoplast preparation of autologous fungus

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References (3)

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Publisher
Springer Journals
Copyright
Copyright © 2017 by Pleiades Publishing, Inc.
Subject
Life Sciences; Biochemistry, general; Microbiology; Medical Microbiology
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/S000368381702003X
Publisher site
See Article on Publisher Site

Abstract

The production of hydrolytic enzymes by the mutant Trichoderma reesei Rut-C30 when cultivated in the presence of various carbon sources: glucose, wheat bran and autoclaved mycelium of Penicillium occitanis CT1 has been studied. Glucose was shown to repress all studied hydrolases, 3% of either wheat bran or autoclaved cell walls led to high titers of enzymes, and were favorably comparable to commercial lysing enzymes (LE). The lysing enzyme cocktail obtained when T. reesei Rut-C30 was cultivated in the presence of autoclaved P. occitanis CT1 mycelia appeared to be a most effective for P. occitanis CT1 protoplast formation. Maximal yield of protoplasts reached 13 × 106 protoplasts/mL while commercial LE preparation released only 4 × 106 protoplasts/mL. The protoplast yield was affected also by the osmotic stabilizer, with KCl giving the best results. Our results suggest that to achieve the best protoplastization rate, the enzyme preparation should be obtained following induction by the autoclaved mycelium of the autologous fungus.

Journal

Applied Biochemistry and Microbiology – Springer Journals

Published: Mar 10, 2017

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