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Authentication of dairy products by immunochemical methods: a review

Authentication of dairy products by immunochemical methods: a review Antibody-based techniques for the assessment of authenticity of dairy products are reviewed in this paper. Because of the inherent complexity of the protein and peptide fractions from milk and even more so from cheese, the use of immunoreagents which are more selective than polyclonal antibodies is usually required for the assaying dairy authenticity by immunochemical methods. Significant advances in this area have been achieved over the last decade thanks to advances in the antipeptide antibody technology, based on the use of properly designed peptides which mimick specified protein substructures as model antigens. Tailor-made antibodies have been developped either for the detection of single protein components or for recognizing protein adducts created by the technological processes employed. Different reagent configurations and immunoassay formats have been devised for a number of analytical applications relevant to the quality control of dairy products, ranging from the monitoring of molecular markers to the tracing of technological processes applied to milk for cheese-making. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Dairy Science & Technology Springer Journals

Authentication of dairy products by immunochemical methods: a review

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Publisher
Springer Journals
Copyright
Copyright © 2011 by INRA and Springer Science+Business Media B.V.
Subject
Chemistry; Agriculture; Food Science ; Microbiology
ISSN
1958-5586
eISSN
1958-5594
DOI
10.1007/s13594-011-0008-7
Publisher site
See Article on Publisher Site

Abstract

Antibody-based techniques for the assessment of authenticity of dairy products are reviewed in this paper. Because of the inherent complexity of the protein and peptide fractions from milk and even more so from cheese, the use of immunoreagents which are more selective than polyclonal antibodies is usually required for the assaying dairy authenticity by immunochemical methods. Significant advances in this area have been achieved over the last decade thanks to advances in the antipeptide antibody technology, based on the use of properly designed peptides which mimick specified protein substructures as model antigens. Tailor-made antibodies have been developped either for the detection of single protein components or for recognizing protein adducts created by the technological processes employed. Different reagent configurations and immunoassay formats have been devised for a number of analytical applications relevant to the quality control of dairy products, ranging from the monitoring of molecular markers to the tracing of technological processes applied to milk for cheese-making.

Journal

Dairy Science & TechnologySpringer Journals

Published: Mar 10, 2011

References