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Australian records of resistance of fungi to fungicides

Australian records of resistance of fungi to fungicides perfect state on PDA. Others produce them best on 30% CULTURE V8 juice agar (8). We initiate all routine cultures from germinated single spores (9). These are obtained by seeding a water agar PRESERVATION We preserve cultures in a viable form by freeze drying. plate (approx. 14 days old) with 1 ml of a suspension of Colonized carnation leaf pieces are placed in ampoules spores in sterile water. Excess water is shaken off and the using aseptic techniques and then freeze dried for 14 plate incubated on the slant for 12 hr. Single germinated hours. Cultures can be initiated directly from the freeze­ spores can then be transferred on a small piece of the dried inoculum. water agar. Cultures of most Fusaria can be forwarded through the All isolates are cultured on PDA slopes and small plates post using colonized carnation leaf pieces dried over (5 cm diam.) of CLA for identification. Sporulation and silica-gel for two days and contained in small envelopes. pigmentation is favoured by light especially near ul­ tra-violet and fluctuating temperature conditions (3,4,7). REFERENCES Cultures are therefore incubated in an alternating (1) Curtis, C. R. (1964) - Physiology of sexual reproduction in temperature regime, 25°C http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Australasian Plant Pathology Springer Journals

Australian records of resistance of fungi to fungicides

Jones, Lewis
Australasian Plant Pathology , Volume 6 (1) – Jan 23, 2011
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References (11)

Publisher
Springer Journals
Copyright
Copyright
Subject
Life Sciences; Plant Pathology; Plant Sciences; Agriculture; Entomology; Ecology
ISSN
0815-3191
eISSN
1448-6032
DOI
10.1071/APP9770013
Publisher site
See Article on Publisher Site

Abstract

perfect state on PDA. Others produce them best on 30% CULTURE V8 juice agar (8). We initiate all routine cultures from germinated single spores (9). These are obtained by seeding a water agar PRESERVATION We preserve cultures in a viable form by freeze drying. plate (approx. 14 days old) with 1 ml of a suspension of Colonized carnation leaf pieces are placed in ampoules spores in sterile water. Excess water is shaken off and the using aseptic techniques and then freeze dried for 14 plate incubated on the slant for 12 hr. Single germinated hours. Cultures can be initiated directly from the freeze­ spores can then be transferred on a small piece of the dried inoculum. water agar. Cultures of most Fusaria can be forwarded through the All isolates are cultured on PDA slopes and small plates post using colonized carnation leaf pieces dried over (5 cm diam.) of CLA for identification. Sporulation and silica-gel for two days and contained in small envelopes. pigmentation is favoured by light especially near ul­ tra-violet and fluctuating temperature conditions (3,4,7). REFERENCES Cultures are therefore incubated in an alternating (1) Curtis, C. R. (1964) - Physiology of sexual reproduction in temperature regime, 25°C

Journal

Australasian Plant Pathology – Springer Journals

Published: Jan 23, 2011

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