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Artificial Reproduction and Embryogeny of the Tiger Frog Hoplobatrachus occipitalis (Günther 1858)

Artificial Reproduction and Embryogeny of the Tiger Frog Hoplobatrachus occipitalis (Günther 1858) The objective of this study was to investigate the embryonic development of the frog Hoplobatrachus occipitalis after their artificial reproduction with the Ovaprim®, a combination of gonadotropin releasing hormone analogue and dopamine antagonist domperidone. Male and female H. occipitalis were collected from the wild and were intramuscularly injected with Ovaprim® at doses of 6 µL/g and 8 µL/g body weight, respectively. The eggs were laid 6 h after hormone injection. The numbers of eggs laid by each female frog were 1726 ± 5.4 (SD) with 94.4% and 88.2% mean fecundity and hatching rate, respectively. The diameter of the eggs at laying was 2.73 ± 0.33 mm while it was 5.04 ± 0.05 mm just before hatching. The stages of embryonic development starting from fertilization to tadpoles’ formation took 27 h to complete at 26.4 ± 0.3 °C. The average height of the tadpoles after hatching was 5.1 mm. Circular tadpole movement in the chorion with an irregular respiratory movement was observed at 21 h after egg-laying. In summary, the study shows that H. occipitalis could reproduce in controlled environment by hormonal induction; the reproductive structures have no significant effect on egg-laying; and the embryonic development time of H. occipitalis differs from that other Anuran species. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Proceedings of the Zoological Society Springer Journals

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Publisher
Springer Journals
Copyright
Copyright © Zoological Society, Kolkata, India 2020
ISSN
0373-5893
eISSN
0974-6919
DOI
10.1007/s12595-020-00341-7
Publisher site
See Article on Publisher Site

Abstract

The objective of this study was to investigate the embryonic development of the frog Hoplobatrachus occipitalis after their artificial reproduction with the Ovaprim®, a combination of gonadotropin releasing hormone analogue and dopamine antagonist domperidone. Male and female H. occipitalis were collected from the wild and were intramuscularly injected with Ovaprim® at doses of 6 µL/g and 8 µL/g body weight, respectively. The eggs were laid 6 h after hormone injection. The numbers of eggs laid by each female frog were 1726 ± 5.4 (SD) with 94.4% and 88.2% mean fecundity and hatching rate, respectively. The diameter of the eggs at laying was 2.73 ± 0.33 mm while it was 5.04 ± 0.05 mm just before hatching. The stages of embryonic development starting from fertilization to tadpoles’ formation took 27 h to complete at 26.4 ± 0.3 °C. The average height of the tadpoles after hatching was 5.1 mm. Circular tadpole movement in the chorion with an irregular respiratory movement was observed at 21 h after egg-laying. In summary, the study shows that H. occipitalis could reproduce in controlled environment by hormonal induction; the reproductive structures have no significant effect on egg-laying; and the embryonic development time of H. occipitalis differs from that other Anuran species.

Journal

Proceedings of the Zoological SocietySpringer Journals

Published: Aug 1, 2020

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