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Application of advanced light microscopic techniques to gain deeper insights into cheese matrix physico-chemistry

Application of advanced light microscopic techniques to gain deeper insights into cheese matrix... Despite considerable advances in confocal microscopy and related technologies, advanced light microscopy techniques have been rarely used to study the microstructure of cheese matrices. Here, we demonstrate the potential of advanced microscopic techniques, namely fluorescence lifetime imaging (FLIM), confocal Raman spectroscopy and non-linear microscopy (two-photon excitation and second harmonic generation microscopy), to study localized physicochemical properties of cheese matrices. These methods allow for precise localization of individual chemical components and help to determine their spatial organization in three dimensions. We discuss both fluorescent labelling and label-free methods that provide valuable information about the localized environment. Overall, these new technologies will enable a greater understanding of the influence of manufacturing processes on cheese quality and consistency. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Dairy Science & Technology Springer Journals

Application of advanced light microscopic techniques to gain deeper insights into cheese matrix physico-chemistry

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Publisher
Springer Journals
Copyright
Copyright © 2015 by INRA and Springer-Verlag France
Subject
Chemistry; Food Science; Agriculture; Microbiology
ISSN
1958-5586
eISSN
1958-5594
DOI
10.1007/s13594-015-0253-2
Publisher site
See Article on Publisher Site

Abstract

Despite considerable advances in confocal microscopy and related technologies, advanced light microscopy techniques have been rarely used to study the microstructure of cheese matrices. Here, we demonstrate the potential of advanced microscopic techniques, namely fluorescence lifetime imaging (FLIM), confocal Raman spectroscopy and non-linear microscopy (two-photon excitation and second harmonic generation microscopy), to study localized physicochemical properties of cheese matrices. These methods allow for precise localization of individual chemical components and help to determine their spatial organization in three dimensions. We discuss both fluorescent labelling and label-free methods that provide valuable information about the localized environment. Overall, these new technologies will enable a greater understanding of the influence of manufacturing processes on cheese quality and consistency.

Journal

Dairy Science & TechnologySpringer Journals

Published: Sep 1, 2015

References