Access the full text.
Sign up today, get DeepDyve free for 14 days.
K.E. Mironova, G.M. Proshkina, A.V. Ryabova, O.A. Stremovskiy, S.A. Lukyanov, R.V. Petrov, S.M. Deyev (2013)
Genetically encoded immunophotosensitizer 4D5scFv-miniSOG is a highly selective agent for targeted photokilling of tumor cells in vitroTheranostics, 3
H.K. Binz, P. Amstutz, A. Kohl, M.T. Stumpp, C. Briand, P. Forrer, M.G. Grütter, A. Plückthun (2004)
High-affinity binders selected from designed ankyrin repeat protein librariesNat. Biotechnol., 22
G. Interlandi, S.K. Wetzel, G. Settanni, A. Plückthun, A. Caflisch (2008)
Characterization and further stabilization of designed ankyrin repeat proteins by combining molecular dynamics simulations and experimentsJ. Mol. Biol., 375
C. Zahnd, M. Kawe, M.T. Stumpp, C. Pasquale, R. Tamaskovic, G. Nagy-Davidescu, B. Dreier, R. Schibli, H.K. Binz, R. Waibel, A. Plückthun (2010)
Efficient tumor targeting with high-affinity designed ankyrin repeat proteins: effects of affinity and molecular sizeCancer Res., 70
I. Gülçin (2012)
Antioxidant activity of food constituents: an overviewArch. Toxicol., 86
D.J. Slamon, G.M. Clark, S.G. Wong, W.J. Levin, A. Ullrich, W.L. McGuire (1987)
Human breast cancer: correlation of relapse and survival with amplification of the HER-2/neu oncogeneScience, 235
A.P. Ryumina, E.O. Serebrovskaya, M.V. Shirmanova, L.B. Snopova, M.M. Kuznetsova, I.V. Turchin, N.I. Ignatova, N.V. Klementieva, A.F. Fradkov, B.E. Shakhov, E.V. Zagaynova, K.A. Lukyanov, S.A. Lukyanov (2013)
Flavoprotein miniSOG as a genetically encoded photosensitizer for cancer cellsBiochim. Biophys. Acta, 1830
S.M. Deyev, E.N. Lebedenko, L.E. Petrovskaya, D.A. Dolgikh, A.G. Gabibov, M.P. Kirpichnikov (2015)
Man-made antibodies and immunoconjugates with desired properties: function optimization using structural engineeringRuss. Chem. Rev., 84
T. Mosmann (1983)
Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assaysJ. Immunol. Methods, 65
T. Finkel (2011)
Signal transduction by reactive oxygen speciesJ. Cell Biol., 194
D. Steiner, P. Forrer, A. Plückthun (2008)
Efficient selection of DARPins with sub-nanomolar affinities using SRP phage displayJ. Mol. Biol., 382
B.A. Gusterson, R.D. Gelber, A. Goldhirsch (1992)
Prognostic importance of c-erbB-2 expression in breast cancerJ. Clin. Oncol., 10
O.N. Shilova, G.M. Proshkina, E.N. Lebedenko, S.M. Deyev (2015)
internalization and recycling of the her2 receptor on human breast adenocarcinoma cells treated with targeted phototoxic protein DARPin-miniSOGActa Nat., 7
A.W. Girotti (2001)
Photosensitized oxidation of membrane lipids: reaction pathwayscytotoxic effects, and cytoprotective mechanisms, J. Photochem. Photobiol., 63
X. Shu, V. Lev-Ram, T.J. Deerinck, Y. Qi, E.B. Ramko, M.W. Davidson, Y. Jin, M.H. Ellisman, R.Y. Tsien (2011)
A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organismsPLoS Biol., 9
Development and functional characterization of novel, high-affinity protein compounds able to selectively kill human cancer cells is an urgent task of modern biomedical research. In this work, we studied the cytotoxicity of a recombinant phototoxic protein DARPin-miniSOG against the HER2-positive human breast adenocarcinoma cells. It was found that targeted phototoxin DARPin-miniSOG interacts specifically with HER2 receptor and causes the light-induced death of HER2-positive cells by the mechanism of necrosis. Irradiation of the cells in the presence of ascorbic acid eliminates the light-induced cytotoxicity of DARPin-miniSOG, which proves the prooxidant mechanism of phototoxin action.
Moscow University Biological Sciences Bulletin – Springer Journals
Published: Apr 28, 2016
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.