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Analysis of Element Composition of DNA-Protein Crystals In Vitro

Analysis of Element Composition of DNA-Protein Crystals In Vitro The universal response of Escherichia coli to stress is enhancing the synthesis of specific histone-like Dps proteins that bind bacterial DNA. As a result, two-dimensional and three-dimensional crystalline arrays can be observed in the cytoplasm of starving bacteria. Conditions for obtaining in vitro co-crystals of DNA-Dps were selected, and their elemental composition was studied using analytical electron microscopy. It was found that Dps in the co-crystal retains its ferritin-like activity; that is, it can stimulate the oxidation of Fe2+ ions to Fe3+ and facilitate the accumulation of iron in the form of Fe2O3 in the inner cavity of the oligomer. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Moscow University Biological Sciences Bulletin Springer Journals

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References (13)

Publisher
Springer Journals
Copyright
Copyright © Allerton Press, Inc. 2019
ISSN
0096-3925
eISSN
1934-791X
DOI
10.3103/S0096392519040102
Publisher site
See Article on Publisher Site

Abstract

The universal response of Escherichia coli to stress is enhancing the synthesis of specific histone-like Dps proteins that bind bacterial DNA. As a result, two-dimensional and three-dimensional crystalline arrays can be observed in the cytoplasm of starving bacteria. Conditions for obtaining in vitro co-crystals of DNA-Dps were selected, and their elemental composition was studied using analytical electron microscopy. It was found that Dps in the co-crystal retains its ferritin-like activity; that is, it can stimulate the oxidation of Fe2+ ions to Fe3+ and facilitate the accumulation of iron in the form of Fe2O3 in the inner cavity of the oligomer.

Journal

Moscow University Biological Sciences BulletinSpringer Journals

Published: Oct 26, 2019

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