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Activity of Neutral Phytase from Obesumbacterium proteus in Recombinant Strains of Yarrowia lipolytica under Cultivation on Low-Grade Vegetable Substrate

Activity of Neutral Phytase from Obesumbacterium proteus in Recombinant Strains of Yarrowia... ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 5, pp. 549–555. © Pleiades Publishing, Inc., 2019. Russian Text © The Author(s), 2019, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2019, Vol. 55, No. 5, pp. 498–505. Activity of Neutral Phytase from Obesumbacterium proteus in Recombinant Strains of Yarrowia lipolytica under Cultivation on Low-Grade Vegetable Substrate a a a b a a, E. G. Serdyuk , E. P. Isakova , N. N. Gessler , E. V. Trubnikova , A. N. Antipov , and Y. I. Deryabina * Bach Institute of Biochemistry, Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences, Moscow, 119071 Russia Kursk State University, Kursk, 305000 Russia *e-mail: yul_der@mail.ru Received December 24, 2018; revised April 14, 2019; accepted April 22, 2019 Abstract—The characteristics of recombinant phytase from Obesumbacterium proteus have been studied after its integration into Yarrowia lipolytica with a Po1f (pUV3-Op) plasmid. Phytase activity was observed in Y. lip- olytica Po1f transformants in a wide pH range (3.5–7.5) and remained at 80% of the initial value after 3-min heating at 95°C, which made it possible to add the enzyme to a fodder produced with heat treatment. In the case of transformant cultivation on low-grade vegetable http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Activity of Neutral Phytase from Obesumbacterium proteus in Recombinant Strains of Yarrowia lipolytica under Cultivation on Low-Grade Vegetable Substrate

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References (5)

Publisher
Springer Journals
Copyright
Copyright © 2019 by Pleiades Publishing, Inc.
Subject
Life Sciences; Biochemistry, general; Microbiology; Medical Microbiology
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/S0003683819050120
Publisher site
See Article on Publisher Site

Abstract

ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 5, pp. 549–555. © Pleiades Publishing, Inc., 2019. Russian Text © The Author(s), 2019, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2019, Vol. 55, No. 5, pp. 498–505. Activity of Neutral Phytase from Obesumbacterium proteus in Recombinant Strains of Yarrowia lipolytica under Cultivation on Low-Grade Vegetable Substrate a a a b a a, E. G. Serdyuk , E. P. Isakova , N. N. Gessler , E. V. Trubnikova , A. N. Antipov , and Y. I. Deryabina * Bach Institute of Biochemistry, Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences, Moscow, 119071 Russia Kursk State University, Kursk, 305000 Russia *e-mail: yul_der@mail.ru Received December 24, 2018; revised April 14, 2019; accepted April 22, 2019 Abstract—The characteristics of recombinant phytase from Obesumbacterium proteus have been studied after its integration into Yarrowia lipolytica with a Po1f (pUV3-Op) plasmid. Phytase activity was observed in Y. lip- olytica Po1f transformants in a wide pH range (3.5–7.5) and remained at 80% of the initial value after 3-min heating at 95°C, which made it possible to add the enzyme to a fodder produced with heat treatment. In the case of transformant cultivation on low-grade vegetable

Journal

Applied Biochemistry and Microbiology – Springer Journals

Published: Sep 25, 2019

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