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A System for Distinguishing Octoploid Strawberry Cultivars Using High-Throughput SNP Genotyping

A System for Distinguishing Octoploid Strawberry Cultivars Using High-Throughput SNP Genotyping Cultivated strawberry (Fragaria × ananassa) is an important commercial berry crop grown throughout the world. Improved strawberry cultivars are developed to meet the needs of consumers and breeders. Strawberries are usually propagated through runners, which sometimes lead to mislabeling or misinterpretation of cultivars. However, perfect identification of strawberry cultivars is essential for germplasm maintenance and for breeding programs. Molecular marker technology has been widely used to distinguish cultivars of other crops, but marker development in octoploid strawberries is complicated. Therefore, SNP marker with high-density and even distribution in the genome has been used currently as efficient DNA markers. In this report, previously published high-quality poly high resolution (PHR) SNPs from the 90 K Axiom® SNP array were utilized to develop a Fluidigm 24 SNPs genotyping system. Hundred nine (109) octoploid strawberry cultivars were screened using this 24 SNPs chip set. In addition, 24 SNPs were mapped to six chromosomes of diploid strawberry (Fragaria vesca). Our developed SNPs fluidigm genotyping is automatable, easy and reliable for processing and interpretation of data. Thus, this high-throughput SNP genotyping system will be a useful tool for distinguishing strawberry cultivars and find out parent-offspring relationship. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Tropical Plant Biology Springer Journals

A System for Distinguishing Octoploid Strawberry Cultivars Using High-Throughput SNP Genotyping

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References (34)

Publisher
Springer Journals
Copyright
Copyright © 2017 by Springer Science+Business Media New York
Subject
Life Sciences; Plant Sciences; Plant Genetics and Genomics; Plant Breeding/Biotechnology; Plant Ecology; Transgenics
ISSN
1935-9756
eISSN
1935-9764
DOI
10.1007/s12042-017-9185-8
Publisher site
See Article on Publisher Site

Abstract

Cultivated strawberry (Fragaria × ananassa) is an important commercial berry crop grown throughout the world. Improved strawberry cultivars are developed to meet the needs of consumers and breeders. Strawberries are usually propagated through runners, which sometimes lead to mislabeling or misinterpretation of cultivars. However, perfect identification of strawberry cultivars is essential for germplasm maintenance and for breeding programs. Molecular marker technology has been widely used to distinguish cultivars of other crops, but marker development in octoploid strawberries is complicated. Therefore, SNP marker with high-density and even distribution in the genome has been used currently as efficient DNA markers. In this report, previously published high-quality poly high resolution (PHR) SNPs from the 90 K Axiom® SNP array were utilized to develop a Fluidigm 24 SNPs genotyping system. Hundred nine (109) octoploid strawberry cultivars were screened using this 24 SNPs chip set. In addition, 24 SNPs were mapped to six chromosomes of diploid strawberry (Fragaria vesca). Our developed SNPs fluidigm genotyping is automatable, easy and reliable for processing and interpretation of data. Thus, this high-throughput SNP genotyping system will be a useful tool for distinguishing strawberry cultivars and find out parent-offspring relationship.

Journal

Tropical Plant BiologySpringer Journals

Published: Mar 24, 2017

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