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A simplified method of DNA extraction from Germinal Membrane of Bladder worm stage of Echinococcus granulosus and its application in disease detection

A simplified method of DNA extraction from Germinal Membrane of Bladder worm stage of... Cystic echinococcosis infection in man and animals are acquired after ingestion of eggs of Echinococcus granulosus. Unilocular fully developed cysts contain protoscoleces within a fluid-filled cavity surrounded by germinal membrane and laminar layers. The cysts are of two types sterile and fertile. In general protoscoleces are used for molecular characterization of strains isolated from fertile cysts. In the present investigation a simple procedure for extraction of DNA from germinal membrane has been described. Further, mitochondrial and nuclear sequence information was compared with the existing sequence information present in the public database. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Proceedings of the Zoological Society Springer Journals

A simplified method of DNA extraction from Germinal Membrane of Bladder worm stage of Echinococcus granulosus and its application in disease detection

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Publisher
Springer Journals
Copyright
Copyright © 2009 by Zoological Society, Kolkata
Subject
Life Sciences; Conservation Biology/Ecology; Biodiversity; Animal Genetics and Genomics; Animal Anatomy / Morphology / Histology; Zoology ; Life Sciences, general
ISSN
0373-5893
eISSN
0974-6919
DOI
10.1007/s12595-009-0010-8
Publisher site
See Article on Publisher Site

Abstract

Cystic echinococcosis infection in man and animals are acquired after ingestion of eggs of Echinococcus granulosus. Unilocular fully developed cysts contain protoscoleces within a fluid-filled cavity surrounded by germinal membrane and laminar layers. The cysts are of two types sterile and fertile. In general protoscoleces are used for molecular characterization of strains isolated from fertile cysts. In the present investigation a simple procedure for extraction of DNA from germinal membrane has been described. Further, mitochondrial and nuclear sequence information was compared with the existing sequence information present in the public database.

Journal

Proceedings of the Zoological SocietySpringer Journals

Published: Oct 22, 2009

References