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A simple method for distinguishing isolates of blood disease bacterium (BDB) from Ralstonia solanacearum through detection of bacteriophage production

A simple method for distinguishing isolates of blood disease bacterium (BDB) from Ralstonia... Blood disease bacterium (BDB) causes major decline of banana plants in many places throughout Indonesia. The cultural and biochemical characteristics of BDB isolates are very similar to those of Ralstonia solanacearum race 2 that causes Moko disease. Therefore, this study aimed to find a simple method for differentiating isolates of BDB from R. solanacearum race 2 of banana by testing their capability to liberate phage. BDB were cultured on sucrose-peptone agar, killed with chloroform vapour then overlaid with agar seeded with indicator strain R484 (R. solanacearum race 2). Twenty-two isolates of BDB from new outbreaks of banana blood disease from Central Java and West Nusa Tengara Provinces and 16 isolates of R. solanacearum race 2 from several countries were tested. All 22 isolates of BDB, but none of the R. solanacearum isolates, were lysogenic, indicating that lysogeny is a highly specific property of BDB and can be used to differentiate BDB from R. solanacearum race 2. The testis simple and can be performed in the laboratory with basic equipment. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Australasian Plant Pathology Springer Journals

A simple method for distinguishing isolates of blood disease bacterium (BDB) from Ralstonia solanacearum through detection of bacteriophage production

Australasian Plant Pathology , Volume 32 (3) – Jan 28, 2011

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Publisher
Springer Journals
Copyright
Copyright © 2003 by Australasian Plant Pathology Society
Subject
Life Sciences; Plant Pathology; Plant Sciences; Agriculture; Entomology; Ecology
ISSN
0815-3191
eISSN
1448-6032
DOI
10.1071/AP03039
Publisher site
See Article on Publisher Site

Abstract

Blood disease bacterium (BDB) causes major decline of banana plants in many places throughout Indonesia. The cultural and biochemical characteristics of BDB isolates are very similar to those of Ralstonia solanacearum race 2 that causes Moko disease. Therefore, this study aimed to find a simple method for differentiating isolates of BDB from R. solanacearum race 2 of banana by testing their capability to liberate phage. BDB were cultured on sucrose-peptone agar, killed with chloroform vapour then overlaid with agar seeded with indicator strain R484 (R. solanacearum race 2). Twenty-two isolates of BDB from new outbreaks of banana blood disease from Central Java and West Nusa Tengara Provinces and 16 isolates of R. solanacearum race 2 from several countries were tested. All 22 isolates of BDB, but none of the R. solanacearum isolates, were lysogenic, indicating that lysogeny is a highly specific property of BDB and can be used to differentiate BDB from R. solanacearum race 2. The testis simple and can be performed in the laboratory with basic equipment.

Journal

Australasian Plant PathologySpringer Journals

Published: Jan 28, 2011

References