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A method for rating sugarcane cultivars for resistance to ratoon stunting disease based on an enzyme-linked immunoassay

A method for rating sugarcane cultivars for resistance to ratoon stunting disease based on an... The evaporative-binding enzyme-linked immunosorbent assay (EB-EIA) was used to compare the relative populations of Leifsonia (Clavibacter) xyli subsp. xyli, the causal agent of ratoon stunting disease, in 25 cultivars of sugarcane that were inoculated by planting them through a contaminated mechanical planter. There were highly significant differences between cultivars in relative populations of bacteria in xylem extracts. However, the percentage of samples rated as positive for the bacterium was consistently lower in only one cultivar, H56–752. The absorbance readings for cultivars between the plant and ratoon crop and between two separate experiments were highly correlated. The EB-EIA is a simple method for rating cultivars for resistance to ratoon stunting disease. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Australasian Plant Pathology Springer Journals

A method for rating sugarcane cultivars for resistance to ratoon stunting disease based on an enzyme-linked immunoassay

Australasian Plant Pathology , Volume 31 (1) – Jan 28, 2011

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References (18)

Publisher
Springer Journals
Copyright
Copyright © 2002 by Australasian Plant Pathology Society
Subject
Life Sciences; Plant Pathology; Plant Sciences; Agriculture; Entomology; Ecology
ISSN
0815-3191
eISSN
1448-6032
DOI
10.1071/AP01067
Publisher site
See Article on Publisher Site

Abstract

The evaporative-binding enzyme-linked immunosorbent assay (EB-EIA) was used to compare the relative populations of Leifsonia (Clavibacter) xyli subsp. xyli, the causal agent of ratoon stunting disease, in 25 cultivars of sugarcane that were inoculated by planting them through a contaminated mechanical planter. There were highly significant differences between cultivars in relative populations of bacteria in xylem extracts. However, the percentage of samples rated as positive for the bacterium was consistently lower in only one cultivar, H56–752. The absorbance readings for cultivars between the plant and ratoon crop and between two separate experiments were highly correlated. The EB-EIA is a simple method for rating cultivars for resistance to ratoon stunting disease.

Journal

Australasian Plant PathologySpringer Journals

Published: Jan 28, 2011

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