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A Kinetic Method for Tungsten Determination in Tungsten-Containing Enzymes

A Kinetic Method for Tungsten Determination in Tungsten-Containing Enzymes A highly sensitive method for tungsten detection in proteins based on the ability of this metal to catalyze the oxidation of rubeanic acid with hydrogen peroxide is described. The method allows the determination of tungsten in protein samples in the concentration range of 0.05 to 0.4 μg/ml. Molybdenum, at a concentration lower than half the concentration of tungsten, as well as iron, selenium, and pterin at concentrations 2.5 times higher than that of tungsten, had no effect on tungsten determination by this method. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

A Kinetic Method for Tungsten Determination in Tungsten-Containing Enzymes

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References (10)

Publisher
Springer Journals
Copyright
Copyright © 2001 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Medical Microbiology; Biochemistry, general; Microbiology
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1023/A:1012319604737
Publisher site
See Article on Publisher Site

Abstract

A highly sensitive method for tungsten detection in proteins based on the ability of this metal to catalyze the oxidation of rubeanic acid with hydrogen peroxide is described. The method allows the determination of tungsten in protein samples in the concentration range of 0.05 to 0.4 μg/ml. Molybdenum, at a concentration lower than half the concentration of tungsten, as well as iron, selenium, and pterin at concentrations 2.5 times higher than that of tungsten, had no effect on tungsten determination by this method.

Journal

Applied Biochemistry and MicrobiologySpringer Journals

Published: Oct 19, 2004

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