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A comparative study of haemocytes from six different snails: Surface staining with lectins and monoclonal antibodies

A comparative study of haemocytes from six different snails: Surface staining with lectins and... Haemocytes taken from six different gastropod snail species,Achatina achatina, A. fulica, Biomphalaria glabrata, Bulinus natalensis 272,Helix aspersa andLymnaea stagnalis, were compared for surface staining with lectins and with monoclonal antibodies raised against haemocytes ofBiomphalaria glabrata andL. stagnalis respectively. The lectin staining did not serve to differentiate haemocyte populations of any species, with haemocytes of all butL. stagnalis staining with concanavalin A, and haemocytes of this species being stained with wheatgerm agglutinin. It was apparent that monoclonal antibodies (Mabs) raised to haemocyte markers of one species of snail were capable of recognising determinants on haemocytes of other snail species. The degree, location and subpopulation specificity of staining differed for the Mabs employed. In particular, Mab Ls8 stained cytoplasmic granules ofL. stagnalis haemocytes, surface structures on haemocytes ofBiomphalaria glabrata, Bulinus natalensis 272,A. achatina andH. aspersa, whereas hameocytes ofA. fulica did not stain. The two most-related snail species, theAchatina spp. showed differential staining patterns with both lectin and antibodies. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Comparative Clinical Pathology Springer Journals

A comparative study of haemocytes from six different snails: Surface staining with lectins and monoclonal antibodies

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References (18)

Publisher
Springer Journals
Copyright
Copyright © 1992 by Springer-Verlag
Subject
Medicine & Public Health; Pathology; Hematology; Oncology
eISSN
1433-2973
DOI
10.1007/BF02984524
Publisher site
See Article on Publisher Site

Abstract

Haemocytes taken from six different gastropod snail species,Achatina achatina, A. fulica, Biomphalaria glabrata, Bulinus natalensis 272,Helix aspersa andLymnaea stagnalis, were compared for surface staining with lectins and with monoclonal antibodies raised against haemocytes ofBiomphalaria glabrata andL. stagnalis respectively. The lectin staining did not serve to differentiate haemocyte populations of any species, with haemocytes of all butL. stagnalis staining with concanavalin A, and haemocytes of this species being stained with wheatgerm agglutinin. It was apparent that monoclonal antibodies (Mabs) raised to haemocyte markers of one species of snail were capable of recognising determinants on haemocytes of other snail species. The degree, location and subpopulation specificity of staining differed for the Mabs employed. In particular, Mab Ls8 stained cytoplasmic granules ofL. stagnalis haemocytes, surface structures on haemocytes ofBiomphalaria glabrata, Bulinus natalensis 272,A. achatina andH. aspersa, whereas hameocytes ofA. fulica did not stain. The two most-related snail species, theAchatina spp. showed differential staining patterns with both lectin and antibodies.

Journal

Comparative Clinical PathologySpringer Journals

Published: Nov 5, 2008

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