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Monika Fellermeier, Maja Raschke, S. Sagner, J. Wungsintaweekul, Christoph Schuhr, S. Hecht, K. Kis, Tanja Radykewicz, P. Adam, F. Rohdich, W. Eisenreich, Adelbert Bacher, D. Arigoni, M. Zenk (2001)
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C-MEC incorporation into isoprenoids of bacteria Species Supplement 14 C-MEC incorporation, % to introduced activity Escherichia coli Without supplements
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Prikl. Biokhim. Mikrobiol
To work out a test system for studying the inhibition of isoprenoid biosynthesis through non-mevalonate pathway, the conversion of 2-14C-methylerythritol 2,4-cyclodiphosphate ( 14 C-MEC) into isoprenoids of three bacteria species and into plastids of 11 higher plant species was studied. The highest efficiency (up to 63%) of the process was observed with chromoplasts from narcissus (Narcissus pseudonarcissus L.) and celandine (Chelidonium majus L.). Twenty five percent of added 14C-MEC was recovered in an isoprenoid fraction of red pepper (Capsicum annuum L.) chromoplasts. Thus, these three models can be used to test antibiotic inhibitors of isoprenoid biosynthesis.
Applied Biochemistry and Microbiology – Springer Journals
Published: Sep 13, 2009
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