Access the full text.
Sign up today, get DeepDyve free for 14 days.
References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.
Abstract.We report energy transfer (ET) from two dyes: Alexa Fluor 514 (AF514) and Alexa Fluor 532 (AF532) to gold nanoparticles (AuNPs) of three different sizes (10, 30, and 53 nm) employing steady-state and time-resolved fluorescence measurements. The results show that the fluorescence intensity and fluorescence lifetimes of donor (D) molecules AF514 and AF532 decrease with increase in the concentration of acceptor (A) AuNPs (2 to 10 μM) upon interaction with AuNPs, thereby confirming the occurrence of ET between D and A. This clearly suggests that these two Alexa Fluor molecules act as efficient donors and AuNPs as excellent acceptors. Interestingly, the Forster distance (Ro) determined for these dyes varies from 212 to 550 Å with increasing size of AuNPs and suggests that the ET from AF514 and AF532 to AuNPs is essentially obeying surface energy transfer (SET) process following 1 / d4 distance dependence. As is well known, Forster resonance energy transfer is efficient for separation distances of up to 100 Å, beyond which its efficiency decreases. Thus, the present results follow dipole-surface type ET from molecule dipole (AF514 and AF532) to nanometal (Au) surface. The influence of size and distance on the SET from AF514 and AF532 to AuNPs is discussed. Further, the quenching of donor fluorescence in the presence of AuNPs and nonradiative ET are analyzed using Stern–Volmer plots. Our study is an experimental quest to explore the potential of such dye–noble metal NPs pairs performing as sensitive chemical and biosensors.
Journal of Nanophotonics – SPIE
Published: Jul 1, 2020
Keywords: Alexa Fluor dyes; gold nanoparticles; surface energy transfer; Stern–Volmer plot
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.