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Detectability of fluorescent gold nanoparticles under micro-CT and optical projection tomography imaging

Detectability of fluorescent gold nanoparticles under micro-CT and optical projection tomography... Abstract.Purpose: Preclinical studies often compare micro-computed tomography (micro-CT) imaging with histology using optical microscopy of fluorescently labeled slides. However, correlating the images is difficult because the tissues appear differently in the two modalities. It would be valuable to have a single contrast medium visible on both radiographic and optical imaging.Approach: We have explored the detectability of fluorescently labeled gold nanoparticles under micro-CT and optical projection tomography (OPT) in agarose phantoms and a murine melanoma tumor model. Murine melanoma cells were used to induce tumor growth in the right hind legs of 12 C57Bl6 mice, with the maximal tumor size of 1  cm3. We injected Cy3 fluorescently coated gold nanorods directly into the tumors. The mice were scanned with in vivo micro-CT (for pre- and post-contrast scans). Once euthanized, the hind leg was dissected and scanned with a higher resolution specimen micro-CT and OPT.Results: The distribution of the gold nanoparticles appeared to be contained and isolated to the tumor. Alignment of micro-CT specimen scans with the OPT scans was possible, although there was also autofluorescence of the surrounding muscle tissue.Conclusions: This study highlights the potential use of fluorescently labeled gold nanoparticles for imaging murine melanoma tumors using micro-CT and OPT. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Medical Imaging SPIE

Detectability of fluorescent gold nanoparticles under micro-CT and optical projection tomography imaging

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Publisher
SPIE
Copyright
© The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
ISSN
2329-4302
eISSN
2329-4310
DOI
10.1117/1.JMI.7.2.026002
Publisher site
See Article on Publisher Site

Abstract

Abstract.Purpose: Preclinical studies often compare micro-computed tomography (micro-CT) imaging with histology using optical microscopy of fluorescently labeled slides. However, correlating the images is difficult because the tissues appear differently in the two modalities. It would be valuable to have a single contrast medium visible on both radiographic and optical imaging.Approach: We have explored the detectability of fluorescently labeled gold nanoparticles under micro-CT and optical projection tomography (OPT) in agarose phantoms and a murine melanoma tumor model. Murine melanoma cells were used to induce tumor growth in the right hind legs of 12 C57Bl6 mice, with the maximal tumor size of 1  cm3. We injected Cy3 fluorescently coated gold nanorods directly into the tumors. The mice were scanned with in vivo micro-CT (for pre- and post-contrast scans). Once euthanized, the hind leg was dissected and scanned with a higher resolution specimen micro-CT and OPT.Results: The distribution of the gold nanoparticles appeared to be contained and isolated to the tumor. Alignment of micro-CT specimen scans with the OPT scans was possible, although there was also autofluorescence of the surrounding muscle tissue.Conclusions: This study highlights the potential use of fluorescently labeled gold nanoparticles for imaging murine melanoma tumors using micro-CT and OPT.

Journal

Journal of Medical ImagingSPIE

Published: Mar 1, 2020

Keywords: gold nanorods; nanoparticles; melanoma; micro-computed tomography; optical projection tomography

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