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Expression of 14-3-3σ in cervical squamous cell carcinomas: Relationship with clinical outcome

Expression of 14-3-3σ in cervical squamous cell carcinomas: Relationship with clinical outcome 14-3-3 sigma (σ) sequesters the cdc2-cyclin B1 complex in the cytoplasm resulting in G2 arrest. Inactivation and reduced expression of 14-3-3σ have been reported in a varity of cancers. In the present study, we investigated the expression of 14-3-3σ in a series of 297 cervical squamous cell carcinoma (SCC) to clarify the prognostic value. Using immunohistochemical methods we found high levels of 14-3-3σ protein in cytoplasm of 143 (48.1%), in nucleus of 113 (38.0%) and in both cytoplasm and nucleus of 147 (49.5%) cases, whereas, low levels were present in cytoplasm of 154 (51.9%), in nucleus of 184 (62.0%) and in both cytoplasm and nucleus of 150 (50.5%) cases. Levels of 14-3-3σ mRNA measured by reverse-transcription polymerase chain reaction (RT-PCR) and 14-3-3σ protein were not significant associated. 14-3-3σ expression in cytoplasm, nuclear and cytoplasm/nuclear were not significantly correlated to disease-specific survival or disease-free survival. In conclusion, reduced expression of 14-3-3σ protein in the cytoplasm and shuttle of 14-3-3σ protein into the nucleus in a relatively high number of cases indicate that 14-3-3σ may be important in the carcinogenesis of cervical SCCs by two different mechanisms; reduction and nuclear translocation of 14-3-3σ protein. Furthermore, the non-significant correlation between expression levels of 14-3-3σ mRNA and protein support a post-transcriptional regulation in cervical SCCs. The protein has no prognostic value in cervical cancers. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Oncology Reports Spandidos Publications

Expression of 14-3-3σ in cervical squamous cell carcinomas: Relationship with clinical outcome

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Publisher
Spandidos Publications
Copyright
Copyright © Spandidos Publications
ISSN
1021-335X
eISSN
1791-2431
DOI
10.3892/or_00000399
Publisher site
See Article on Publisher Site

Abstract

14-3-3 sigma (σ) sequesters the cdc2-cyclin B1 complex in the cytoplasm resulting in G2 arrest. Inactivation and reduced expression of 14-3-3σ have been reported in a varity of cancers. In the present study, we investigated the expression of 14-3-3σ in a series of 297 cervical squamous cell carcinoma (SCC) to clarify the prognostic value. Using immunohistochemical methods we found high levels of 14-3-3σ protein in cytoplasm of 143 (48.1%), in nucleus of 113 (38.0%) and in both cytoplasm and nucleus of 147 (49.5%) cases, whereas, low levels were present in cytoplasm of 154 (51.9%), in nucleus of 184 (62.0%) and in both cytoplasm and nucleus of 150 (50.5%) cases. Levels of 14-3-3σ mRNA measured by reverse-transcription polymerase chain reaction (RT-PCR) and 14-3-3σ protein were not significant associated. 14-3-3σ expression in cytoplasm, nuclear and cytoplasm/nuclear were not significantly correlated to disease-specific survival or disease-free survival. In conclusion, reduced expression of 14-3-3σ protein in the cytoplasm and shuttle of 14-3-3σ protein into the nucleus in a relatively high number of cases indicate that 14-3-3σ may be important in the carcinogenesis of cervical SCCs by two different mechanisms; reduction and nuclear translocation of 14-3-3σ protein. Furthermore, the non-significant correlation between expression levels of 14-3-3σ mRNA and protein support a post-transcriptional regulation in cervical SCCs. The protein has no prognostic value in cervical cancers.

Journal

Oncology ReportsSpandidos Publications

Published: Jul 1, 2009

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