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DNA image cytometry on sections compared with flow cytometry in human bone metastases

DNA image cytometry on sections compared with flow cytometry in human bone metastases The DNA analysis of tumor cells discloses characteristic features from which their biological behavior with respect to both the intensity and regulation of growth can be deduced. The objective of this study was to comparatively assess the results of image cytometry (ICM) and flow cytometry (FCM) in human bone metastases as standard methods of DNA analysis and evaluate their possible importance. The nuclear DNA content of surgically removed tumors of bone tissue was determined using ICM and FCM, and the percentage of tumor cells in various cell cycle phases and ploidy status in each case were determined based on the DNA distribution pattern. Comparable results were determined by ICM and FCM with respect to the ploidy status in about 58% of examined tumor samples. When tissue samples from various regions of a tumor were examined, it was found that DNA-euploid and -aneuploid tumor areas were present within the tumors. The DNA aneuploidy was detected in 90% of these tumors with ICM. The percentage cell-cycle phase distribution varied widely with ICM and FCM. Based on our results, the use of ICM in addition to FCM is mandatory under certain conditions for the assessment of the DNA analysis of bone metastases and necessary for the critical assessment of the obtained findings. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Oncology Reports Spandidos Publications

DNA image cytometry on sections compared with flow cytometry in human bone metastases

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Publisher
Spandidos Publications
Copyright
Copyright © Spandidos Publications
ISSN
1021-335X
eISSN
1791-2431
DOI
10.3892/or.14.4.1005
Publisher site
See Article on Publisher Site

Abstract

The DNA analysis of tumor cells discloses characteristic features from which their biological behavior with respect to both the intensity and regulation of growth can be deduced. The objective of this study was to comparatively assess the results of image cytometry (ICM) and flow cytometry (FCM) in human bone metastases as standard methods of DNA analysis and evaluate their possible importance. The nuclear DNA content of surgically removed tumors of bone tissue was determined using ICM and FCM, and the percentage of tumor cells in various cell cycle phases and ploidy status in each case were determined based on the DNA distribution pattern. Comparable results were determined by ICM and FCM with respect to the ploidy status in about 58% of examined tumor samples. When tissue samples from various regions of a tumor were examined, it was found that DNA-euploid and -aneuploid tumor areas were present within the tumors. The DNA aneuploidy was detected in 90% of these tumors with ICM. The percentage cell-cycle phase distribution varied widely with ICM and FCM. Based on our results, the use of ICM in addition to FCM is mandatory under certain conditions for the assessment of the DNA analysis of bone metastases and necessary for the critical assessment of the obtained findings.

Journal

Oncology Reports – Spandidos Publications

Published: Oct 1, 2005

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