Access the full text.
Sign up today, get DeepDyve free for 14 days.
Hyenjong Hong, Kazutoshi Takahashi, T. Ichisaka, T. Aoi, O. Kanagawa, M. Nakagawa, K. Okita, S. Yamanaka (2009)
Suppression of induced pluripotent stem cell generation by the p53–p21 pathwayNature, 460
G. Dontu, W. Abdallah, J. Foley, Kyle Jackson, M. Clarke, M. Kawamura, M. Wicha (2003)
In vitro propagation and transcriptional profiling of human mammary stem/progenitor cells.Genes & development, 17 10
Isabel Chu, L. Hengst, J. Slingerland (2008)
The Cdk inhibitor p27 in human cancer: prognostic potential and relevance to anticancer therapyNature Reviews Cancer, 8
Shengyong Yin, Jinjun Li, Chen Hu, Xinhua Chen, M. Yao, Ming-xia Yan, G. Jiang, Chao Ge, Haiyang Xie, D. Wan, Shengli Yang, Shusen Zheng, J. Gu (2007)
CD133 positive hepatocellular carcinoma cells possess high capacity for tumorigenicityInternational Journal of Cancer, 120
S. Ma, T. Lee, B. Zheng, K. Chan, X. Guan (2008)
CD133+ HCC cancer stem cells confer chemoresistance by preferential expression of the Akt/PKB survival pathwayOncogene, 27
T. Yamashita, Junfang Ji, A. Budhu, M. Forgues, Wen Yang, Hong-Yang Wang, H. Jia, Q. Ye, L. Qin, E. Wauthier, L. Reid, H. Minato, M. Honda, S. Kaneko, Zhao-You Tang, X. Wang (2009)
EpCAM-positive hepatocellular carcinoma cells are tumor-initiating cells with stem/progenitor cell features.Gastroenterology, 136 3
O. Coqueret (2003)
New roles for p21 and p27 cell-cycle inhibitors: a function for each cell compartment?Trends in cell biology, 13 2
(2005)
The role of hypoxiainducible factor-1 in three-dimensional tumor growth, apoptosis and regulation by the insulin-signaling pathway
(2008)
Significance of CD 90 +
Z. Yang, D. Ho, M. Ng, C. Lau, W. Yu, P. Ngai, P. Chu, C. Lam, R. Poon, S. Fan (2008)
Significance of CD90+ cancer stem cells in human liver cancer.Cancer cell, 13 2
T. Chiba, Kaoru Kita, Yun-Wen Zheng, O. Yokosuka, H. Saisho, A. Iwama, H. Nakauchi, H. Taniguchi (2006)
Side population purified from hepatocellular carcinoma cells harbors cancer stem cell–like propertiesHepatology, 44
Y. Yamashita, M. Shimada, N. Harimoto, Shinji Tanaka, K. Shirabe, H. Ijima, K. Nakazawa, J. Fukuda, K. Funatsu, Y. Maehara (2004)
cDNA Microarray Analysis in Hepatocyte Differentiation in Huh 7 CellsCell Transplantation, 13
Z. Yang, P. Ngai, D. Ho, W. Yu, M. Ng, C. Lau, Mandy Li, K. Tam, C. Lam, R. Poon, S. Fan (2008)
Identification of local and circulating cancer stem cells in human liver cancerHepatology, 47
Yasuhiro Ito, N. Matsuura, M. Sakon, E. Miyoshi, K. Noda, T. Takeda, K. Umeshita, Hiroyuki Nagano, S. Nakamori, K. Dono, M. Tsujimoto, M. Nakahara, K. Nakao, Naoyuki Taniguchi, M. Monden (1999)
Expression and prognostic roles of the G1‐S modulators in hepatocellular carcinoma: p27 independently predicts the recurrenceHepatology, 30
L. Vermeulen, M. Sprick, K. Kemper, G. Stassi, J. Medema (2008)
Cancer stem cells – old concepts, new insightsCell Death and Differentiation, 15
W. Weinberg, M. Denning (2002)
P21Waf1 control of epithelial cell cycle and cell fate.Critical reviews in oral biology and medicine : an official publication of the American Association of Oral Biologists, 13 6
Y. Zen, T. Fujii, S. Yoshikawa, H. Takamura, T. Tani, T. Ohta, Y. Nakanuma (2007)
Histological and culture studies with respect to ABCG2 expression support the existence of a cancer cell hierarchy in human hepatocellular carcinoma.The American journal of pathology, 170 5
C. Sherr, James Roberts (1999)
CDK inhibitors: positive and negative regulators of G1-phase progression.Genes & development, 13 12
(2005)
Establishment of a doxycyclineregulated cell line with inducible, doubly-stable expression of the wild-type p53 gene from p53-deleted hepatocellular carcinoma cells
A. Besson, S. Dowdy, James Roberts (2008)
CDK inhibitors: cell cycle regulators and beyond.Developmental cell, 14 2
Sendurai Mani, Wenjun Guo, Mai-Jing Liao, E. Eaton, Ayyakkannu Ayyanan, A. Zhou, M. Brooks, Ferenc Reinhard, C. Zhang, M. Shipitsin, Lauren Campbell, K. Polyak, C. Brisken, Jing Yang, R. Weinberg (2008)
The Epithelial-Mesenchymal Transition Generates Cells with Properties of Stem CellsCell, 133
H. Xing, Shixuan Wang, Ke Hu, Wenming Tao, Jing Li, Qing-mei Gao, Xiaokui Yang, D. Weng, Yunping Lu, D. Ma (2005)
Effect of the cyclin-dependent kinases inhibitor p27 on resistance of ovarian cancer multicellular spheroids to anticancer chemotherapyJournal of Cancer Research and Clinical Oncology, 131
(2009)
Linking the p53 tumour suppressor pathway to somatic cell reprogramming
Arihiro Aihara, Shinji Tanaka, M. Yasen, S. Matsumura, Y. Mitsunori, A. Murakata, N. Noguchi, A. Kudo, N. Nakamura, Koji Ito, S. Arii (2010)
The selective Aurora B kinase inhibitor AZD1152 as a novel treatment for hepatocellular carcinoma.Journal of hepatology, 52 1
Y. Kamohara, N. Haraguchi, K. Mimori, F. Tanaka, H. Inoue, M. Mori, T. Kanematsu (2008)
The search for cancer stem cells in hepatocellular carcinoma.Surgery, 144 2
A. Tannapfel, Dorothee Grund, A. Katalinic, D. Uhlmann, F. Köckerling, U. Haugwitz, M. Wasner, J. Hauss, K. Engeland, C. Wittekind (2000)
Decreased expression of p27 protein is associated with advanced tumor stage in hepatocellular carcinomaInternational Journal of Cancer, 89
Angelo Cicalese, G. Bonizzi, C. Pasi, M. Faretta, S. Ronzoni, B. Giulini, C. Brisken, S. Minucci, P. Fiore, P. Pelicci (2009)
The Tumor Suppressor p53 Regulates Polarity of Self-Renewing Divisions in Mammary Stem CellsCell, 138
Zhenbiao Zhu, Xiangfang Hao, Ming-xia Yan, M. Yao, Chao Ge, J. Gu, Jinjun Li (2009)
Cancer stem/progenitor cells are highly enriched in CD133+CD44+ population in hepatocellular carcinomaInternational Journal of Cancer, 126
(2004)
Microenviromental regulation of proliferation in multicellular spheroids is mediated through differential expression of cyclin-dependent kinase inhibitors
T. Reya, S. Morrison, M. Clarke, I. Weissman (2001)
Stem cells, cancer, and cancer stem cellsNature, 414
Increasing evidence suggests that cancers contain a small subset of cancer-initiating cells, so-called cancer stem cells (CSCs) that are capable of regenerating a tumor after chemoradiation therapy. Sphere forming ability is known to be one of properties of CSCs, but the significance remains unclear. The present study focused on sphere formation of human hepatoma cells in three-dimensional culture in order to evaluate the analogy between sphere forming ability and stemness of cancer cells in vitro. Under three-dimensional culture condition, HepG2, Hep3B and PLC/PRF/5 cells demonstrated the sphere formation while SK-Hep1 and Huh-7 cells did not. The population of G0/G1 phase increased in the spheres compared with the monolayer (67 vs. 38%). In spite of no significant difference in stem cell surface markers (CD44, CD90, CD133, EpCAM and ABCG2), remarkable up-regulation of p27 CDK inhibitor was observed in sphere forming cells. Immunofluorescence analysis revealed the nuclear expression of p27 in the whole of the sphere, but weak expression of p21 only at the peripheral area. The spheres acquired chemoresistance to cisplatin compared with the monolayers (58.9 vs. 16.2 µM in IC50). This model was useful for assessment of the role of cell-cycle quiescence in the stemness and chemoresistance of cancer cells.
Oncology Reports – Spandidos Publications
Published: Nov 1, 2010
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.