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Toxicity of Detergent-based Commercial Products on Cells of a Mouse Line in Suspension Culture as a Possible Screen for the Draize Rabbit Eye Irritation Test

Toxicity of Detergent-based Commercial Products on Cells of a Mouse Line in Suspension Culture as... SummaryThe Draize rabbit eye irritation test has several disadvantages and inadequacies when used as an indicator for potential irritancy of detergent-based commercial products. As a possible in vitro screen, it was decided to use mouse LS cells cultured in suspension, taking 50% cell death (CD50) after exposure to the product for 4h as the endpoint. This figure for 11 formulations was compared with eye irritation data ranked using an arbitrary classification of mild, moderate and severe. All samples with a CD50 less than 0.5mg/ml were severe eye irritants, while most of those with a CD50 greater than 1.0mg/ml were mild irritants. Samples between these two cytotoxicity levels were, in general, moderately irritant to the rabbit eye.It would appear that this in vitro test is a possible screen for the irritancy of detergent-based products. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Alternatives to Laboratory Animals SAGE

Toxicity of Detergent-based Commercial Products on Cells of a Mouse Line in Suspension Culture as a Possible Screen for the Draize Rabbit Eye Irritation Test

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References (16)

Publisher
SAGE
Copyright
© 1983 Fund for the Replacement of Animals in Medical Experiments
ISSN
0261-1929
eISSN
2632-3559
DOI
10.1177/026119298301100104
Publisher site
See Article on Publisher Site

Abstract

SummaryThe Draize rabbit eye irritation test has several disadvantages and inadequacies when used as an indicator for potential irritancy of detergent-based commercial products. As a possible in vitro screen, it was decided to use mouse LS cells cultured in suspension, taking 50% cell death (CD50) after exposure to the product for 4h as the endpoint. This figure for 11 formulations was compared with eye irritation data ranked using an arbitrary classification of mild, moderate and severe. All samples with a CD50 less than 0.5mg/ml were severe eye irritants, while most of those with a CD50 greater than 1.0mg/ml were mild irritants. Samples between these two cytotoxicity levels were, in general, moderately irritant to the rabbit eye.It would appear that this in vitro test is a possible screen for the irritancy of detergent-based products.

Journal

Alternatives to Laboratory AnimalsSAGE

Published: Sep 1, 1983

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