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Zhanjiang Liu, J. Cordes (2004)
DNA marker technologies and their applications in aquaculture genetics.Aquaculture, 238
T. Borodina, H. Lehrach, A. Soldatov (2004)
Ligation detection reaction-TaqMan procedure for single nucleotide polymorphism detection on genomic DNA.Analytical biochemistry, 333 2
Kyoko Takatsu, T. Yokomaku, S. Kurata, T. Kanagawa (2004)
A new approach to SNP genotyping with fluorescently labeled mononucleotides.Nucleic acids research, 32 7
B. Rosenzweig, P. Pine, O. Domon, Suzanne Morris, James Chen, F. Sistare (2004)
Dye bias correction in dual-labeled cDNA microarray gene expression measurements.Environmental Health Perspectives, 112
O. Iakoubova, C. Olsson, K. Dains, J. Choi, I. Kalcheva, L. Bentley, M. Cunanan, D. Hillman, J. Louie, M. Machrus, D. West (2000)
Microsatellite marker panels for use in high-throughput genotyping of mouse crosses.Physiological genomics, 3 3
Florence Mauger, Olivier Jaunay, V. Chamblain, F. Reichert, Keith Bauer, I. Gut, D. Gelfand (2006)
SNP genotyping using alkali cleavage of RNA/DNA chimeras and MALDI time-of-flight mass spectrometryNucleic Acids Research, 34
Takahiro Sakai, Y. Kikkawa, I. Miura, Takeshi Inoue, K. Moriwaki, T. Shiroishi, Y. Satta, N. Takahata, H. Yonekawa (2004)
Origins of mouse inbred strains deduced from whole-genome scanning by polymorphic microsatellite lociMammalian Genome, 16
W. Russell, R. Burch (1960)
The Principles of Humane Experimental TechniqueMedical Journal of Australia, 1
N. Tintle, K. Ahn, N. Mendell, D. Gordon, S. Finch (2005)
Characteristics of replicated single-nucleotide polymorphism genotypes from COGA: Affymetrix and Center for Inherited Disease ResearchBMC Genetics, 6
S. Kang, D. Gordon, A. Brown, J. Ott, S. Finch (2003)
Tradeoff Between No-Call Reduction in Genotyping Error Rate and Loss of Sample Size for Genetic Case/Control Association StudiesPacific Symposium on Biocomputing. Pacific Symposium on Biocomputing
D. Alves, R. Pereira, S. Leal-Bertioli, M. Moretzsohn, P. Guimaraes, D. Bertioli (2008)
Development and use of single nucleotide polymorphism markers for candidate resistance genes in wild peanuts (Arachis spp).Genetics and molecular research : GMR, 7 3
K. Lindblad-Toh, E. Winchester, M. Daly, David Wang, J. Hirschhorn, Jean-Philippe Laviolette, K. Ardlie, D. Reich, Elizabeth Robinson, P. Sklar, Nila Shah, D. Thomas, Jian-Bing Fan, T. Gingeras, J. Warrington, N. Patil, T. Hudson, E. Lander (2000)
Large-scale discovery and genotyping of single-nucleotide polymorphisms in the mouseNature Genetics, 24
M. Festing (2009)
Improving the Design and Analysis of Animal Experiments: A Personal OdysseyAlternatives to Laboratory Animals, 37
K. Hao, Xiaobin Wang (2005)
Incorporating Individual Error Rate into Association Test of Unmatched Case-Control DesignHuman Heredity, 58
Meiju Ji, P. Hou, Song Li, N. He, Zuhong Lu (2004)
Microarray-based method for genotyping of functional single nucleotide polymorphisms using dual-color fluorescence hybridization.Mutation research, 548 1-2
A. Flavell, V. Bolshakov, A. Booth, R. Jing, J. Russell, T. Ellis, P. Isaac (2003)
A microarray-based high throughput molecular marker genotyping method: the tagged microarray marker (TAM) approach.Nucleic acids research, 31 19
S. Tsang, Zhonghe Sun, B. Luke, C. Stewart, N. Lum, Melissa Gregory, Xiaolin Wu, M. Subleski, N. Jenkins, N. Copeland, D. Munroe (2005)
A comprehensive SNP-based genetic analysis of inbred mouse strainsMammalian Genome, 16
P. Petkov, Yueming Ding, Megan Cassell, Weidong Zhang, Gunjan Wagner, E. Sargent, S. Asquith, Victor Crew, Kevin Johnson, P. Robinson, V. Scott, M. Wiles (2004)
An efficient SNP system for mouse genome scanning and elucidating strain relationships.Genome research, 14 9
C. Wade, E. Kulbokas, Andrew Kirby, M. Zody, J. Mullikin, E. Lander, K. Lindblad-Toh, M. Daly (2002)
The mosaic structure of variation in the laboratory mouse genomeNature, 420
Ensuring the genetic homogeneity of the mice used in laboratory experiments contributes to the Reduction aspect of the Three Rs, by maximising the quality of the data obtained from any animals that are used for these purposes, and ultimately reducing the numbers of animals used. Single nucleotide polymorphism (SNP) genotyping is especially suitable for use in the analysis of the genetic purity of model organisms such as the mouse, because bi-allelic markers remain fully informative when used to characterise crosses between inbred strains. Here, we attempted to apply a microarray-based method for a SNP marker to monitor the genetic quality of inbred mouse strains, so as to validate the reliability, stability and applicability of this SNP genotyping panel. The amplified PCR products containing four different SNP loci from four inbred mouse strains were spotted and immobilised onto amino-modified glass slides to generate a microarray. This was then interrogated through hybridisation with dual-colour probes, to determine the SNP genotypes of each sample. The results indicated that this microarray-based method could effectively determine the genotypes of the four selected SNPs with a high degree of accuracy. We have developed a new SNP genotyping technique for effective use in the genetic monitoring of inbred mouse strains.
Alternatives to Laboratory Animals – SAGE
Published: Jul 1, 2012
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