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ATLA 38, 443–448, 2010 443 minor) has been proposed as an ideal host for the An Ex Vivo Prostate Gland Model study of bacterial pathogenesis. Traditionally, mammalian in vivo models have been the most Human prostate tissue has been employed to common choice, but scientists are increasingly investigate how different cell types respond to relying on alternative models such as DNA damage. While mice and cell lines have Caenorhabditis elegans, Danio rerio and been the usual models for this type of research, Drosophila. This novel model system has recently the use of human tissue is a promising alter- been used to study human pathogenic infections. native approach. In this study, duckweed was used as the host, In order to develop and optimise the technique, and Pseudomonas aeruginosa and Staphylo - prostate tissue, donated from patients that had coccus aureus were the model pathogens. undergone prostate gland surgery, was used. The Previous studies have shown that pathogenic tissue was cut into thin slices to allow nutrient bacteria employ a similar set of virulence factors and gaseous exchange to take place, and was to infect and cause disease in both plants and stored in a liquid medium that supported its bio-
Alternatives to Laboratory Animals – SAGE
Published: Dec 1, 2010
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