Access the full text.
Sign up today, get DeepDyve free for 14 days.
A. Hansson, B. Bloor, Z. Sarang, Y. Haig, P. Morgan, H. Stark, N. Fusenig, J. Ekstrand, R. Grafström (2003)
Analysis of proliferation, apoptosis and keratin expression in cultured normal and immortalized human buccal keratinocytes.European journal of oral sciences, 111 1
K. Sundqvist, P. Kulkarni, S. Hybbinette, F. Bertolero, Y. Liu, R. Grafström (1991)
Serum-free growth and karyotype analyses of cultured normal and tumorous (SqCC/Y1) human buccal epithelial cells.Cancer communications, 3 10-11
Julie Daniels, J. Kearney, Eileen Ingham (1997)
An investigation into the potential of extracellular matrix factors for attachment and proliferation of human keratinocytes on skin substitutes.Burns : journal of the International Society for Burn Injuries, 23 1
Raj Mallipeddi (2002)
Epidermolysis bullosa and cancerClinical and Experimental Dermatology, 27
M. Birchall, C. Winterford, L. Tripconi, G. Gobe, B. Harmon (1996)
Apoptosis and mitosis in oral and oropharyngeal epithelia: evidence for a topographical switch in premalignant lesionsCell Proliferation, 29
K. Brown, E. Parkinson (1984)
Extracellular matrix components produced by SV40‐transformed human epidermal keratinocytesInternational Journal of Cancer, 33
F. Décline, O. Okamoto, F. Mallein-Gerin, B. Helbert, J. Bernaud, D. Rigal, P. Rousselle (2003)
Keratinocyte motility induced by TGF-beta1 is accompanied by dramatic changes in cellular interactions with laminin 5.Cell motility and the cytoskeleton, 54 1
W. Friess (1998)
Collagen – biomaterial for drug delivery 1
J. Uitto, L. Pulkkinen (2004)
Molecular complexity of the cutaneous basement membrane zoneMolecular Biology Reports, 23
L. Koivisto, K. Larjava, L. Häkkinen, V. Uitto, J. Heino, H. Larjava (1999)
Different integrins mediate cell spreading, haptotaxis and lateral migration of HaCaT keratinocytes on fibronectin.Cell adhesion and communication, 7 3
R. Hynes (2002)
Integrins Bidirectional, Allosteric Signaling MachinesCell, 110
R. Sanders, F. Mainiero, F. Giancotti (1998)
The role of integrins in tumorigenesis and metastasis.Cancer investigation, 16 5
J. Nilsson, X. Zheng, K. Sundqvist, Y. Liu, L. Atzori, A. Elfwing, K. Arvidson, R. Grafström (1998)
Toxicity of Formaldehyde to Human Oral Fibroblasts and Epithelial Cells: Influences of Culture Conditions and Role of Thiol StatusJournal of Dental Research, 77
E. Kucharz (1992)
The Collagens: Biochemistry and Pathophysiology
E. Plow, T. Haas, Li Zhang, J. Loftus, Jeffrey Smith (2000)
Ligand Binding to Integrins*The Journal of Biological Chemistry, 275
(1983)
Establishment and characterisation of a human squamous head and neck cancer cell line
G. Thomas, J. Jones, P. Speight (1997)
Integrins and oral cancer.Oral oncology, 33 6
G. Thomas, P. Speight (2001)
Cell adhesion molecules and oral cancer.Critical reviews in oral biology and medicine : an official publication of the American Association of Oral Biologists, 12 6
E. O’Toole (2001)
Extracellular matrix and keratinocyte migrationClinical and Experimental Dermatology, 26
J. Myllyharju, K. Kivirikko (2001)
Collagens and collagen-related diseasesAnnals of Medicine, 33
A. Hansson, B. Bloor, Y. Haig, P. Morgan, J. Ekstrand, R. Grafström (2001)
Expression of keratins in normal, immortalized and malignant oral epithelia in organotypic culture.Oral oncology, 37 5
E. Ruoslahti (1999)
Fibronectin and its integrin receptors in cancer.Advances in cancer research, 76
R. Hynes (1990)
Fibronectins
K. Brown, E. Parkinson (1985)
Alteration of the extracellular matrix of cultured human keratinocytes by transformation and during differentiationInternational Journal of Cancer, 35
D. Woodley, J. Chen, J. Kim, Y. Sarret, T. Iwasaki, Y. Kim, E. O'Keefe (1993)
Re-epithelialization. Human keratinocyte locomotion.Dermatologic clinics, 11 4
J. Hedberg, R. Grafström, M. Vondráček, Z. Sarang, L. Wärngård, J. Höög (2001)
Micro-array chip analysis of carbonyl-metabolising enzymes in normal, immortalised and malignant human oral keratinocytesCellular and Molecular Life Sciences CMLS, 58
(2002)
Integrins: bidirectional, allosteric signaling
J. Hedberg, A. Hansson, J. Nilsson, J. Höög, R. Grafström (2001)
Uniform Expression of Alcohol Dehydrogenase 3 in Epithelia Regenerated with Cultured Normal, Immortalised and Malignant Human Oral KeratinocytesAlternatives to Laboratory Animals, 29
D. Dressler, Z. Sarang, Z. Szondy, K. Engelhart, R. Grafström (2002)
Expression of retinoid-related genes in serum-free cultures of normal, immortalized and malignant human oral keratinocytes.International journal of oncology, 20 5
P. Kulkarni, K. Sundqvist, C. Betsholtz, P. Höglund, K. Wiman, B. Zhivotovsky, F. Bertolero, Y. Liu, R. Grafström (1995)
Characterization of human buccal epithelial cells transfected with the simian virus 40 T-antigen gene.Carcinogenesis, 16 10
(1995)
Characterisation of human buccal epithelial cells transfected with the simian virus
R. Grafström (2002)
Human Oral Epithelium
B. Pilcher, B. Sudbeck, J. Dumin, H. Welgus, W. Parks (1998)
Collagenase-1 and collagen in epidermal repairArchives of Dermatological Research, 290
M. Vondráček, D. Weaver, Z. Sarang, J. Hedberg, J. Willey, L. Wärngård, R. Grafström (2002)
Transcript profiling of enzymes involved in detoxification of xenobiotics and reactive oxygen in human normal and simian virus 40 T antigen‐immortalized oral keratinocytesInternational Journal of Cancer, 99
T. Jussila, S. Kauppila, L. Risteli, J. Risteli, F. Stenbäck (2002)
Collagen formation in extracellular matrix of transplants of human transformed keratinocyte cell lines.Anticancer research, 22 3
A. Brazma, J. Vilo (2000)
Gene expression data analysisFEBS Letters, 480
L. Hudson, L. Mccawley (1998)
Contributions of the epidermal growth factor receptor to keratinocyte motilityMicroscopy Research and Technique, 43
B. Ziober, Sol Silverman, R. Kramer (2001)
Adhesive mechanisms regulating invasion and metastasis in oral cancer.Critical reviews in oral biology and medicine : an official publication of the American Association of Oral Biologists, 12 6
R. Grafström, U. Norén, X. Zheng, A. Elfwing, K. Sundqvist (1997)
Growth and transformation of human oral epithelium in vitro.Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer, 143
R. Grafström (1990)
Carcinogenesis studies in human epithelial tissues and cells in vitro: emphasis on serum-free culture conditions and transformation studies.Acta physiologica Scandinavica. Supplementum, 592
Extracellular matrix proteins affect the growth and survival of epithelial tissues. Accordingly, surface coating with fibronectin and collagen is a common practice for promoting keratinocyte culture. In this study, the expression of fibronectin and collagen-related factors, including integrins, by normal (NOK), SV40 T-antigen-immortalised (SVpgC2a) and malignant (SqCC/Y1) human oral keratinocytes, under standardised, serum-free conditions, was investigated by using microarray analysis. Cell growth was also studied in the presence and absence of a matrix consisting of human fibronectin and bovine collagen type I (FN–COL). Fibronectin transcripts were abundant in all cells, whereas 16 of 29 collagen chains and 14 of 24 integrin subunits were variably detected. With regard to both the expression level and the number of transcripts, higher collagen and lower integrin expression was observed in SVpgC2a cells than in NOKs and SqCC/Y1 cells. The cell types differed with regard to colony-forming efficiency and the rate and kinetics of growth at high cell density. For all cell types, FN–COL coating consistently stimulated cell migration, without influencing growth in mass culture or clonal density. The results demonstrate the transcription of genes associated with the formation and function of fibronectin and collagen in oral epithelium, and variably altered expression patterns in transformed states, and show that keratinocyte lines can be successfully transferred without the stimulus from extracellular FN–COL.
Alternatives to Laboratory Animals – SAGE
Published: Nov 1, 2003
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.