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Buchanan, M. Vázquez, M. Gimbrone (1983)
Arachidonic acid metabolism and the adhesion of human polymorphonuclear leukocytes to cultured vascular endothelial cellsBlood, 62
Joyce-Loebl (1985)
Image Analysis: Principles and Practice
J. Folkman, C. Haudenschild, B. Zetter (1979)
Long-term culture of capillary endothelial cells.Proceedings of the National Academy of Sciences of the United States of America, 76 10
M. Tonnesen, L. Smedly, P. Henson (1984)
Neutrophil-endothelial cell interactions. Modulation of neutrophil adhesiveness induced by complement fragments C5a and C5a des arg and formyl-methionyl-leucyl-phenylalanine in vitro.The Journal of clinical investigation, 74 5
Hoover R.L. (1980)
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R. Hoover, R. Folger, W. Haering, B. Ware, M. Karnovsky (1980)
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Quantitative investigations of the adhesiveness of circulating polymorphonuclear leucocytes to blood vessel wallsThe Journal of Physiology, 222
Polymorphonuclear neutrophil (PMN) interaction with vascular endothelial cells is the initial event in the migration of neutrophils through blood vessel walls before reaching inflammation sites in tissues. The interaction between fibroblasts and endothelial cells and their extracellular matrices might be modulated by the activation of neutrophils that occurs at inflammatory reaction sites. We have used an in vitro model to study PMN function, measuring the adhesion of human PMNs to capillary endothelial cells and fibroblasts grown in culture and to their extracellular matrices. The interaction was measured in basal conditions and in the presence of the chemotactic effector, formyl-methionyl-leucyl-phenylalanine (FMLP at the concentration of 10 7M). Adhesion was expressed by the number of adherent PMNs/mm2 on a histological specimen. Moreover, we have adapted a program for image analysis to quantify neutrophil adhesion. Three times more PMNs adhered to matrices than to monolayers, and adherence could be increased by the presence of 10-7M FMLP, except in the case of fibroblast monolayers. We found a good correlation between microscopic observation and computerised image analysis measuring PMN adhesiveness to extracellular matrices.
Alternatives to Laboratory Animals – SAGE
Published: Sep 1, 1988
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