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A. Ghanem, M. Shuler (2000)
Characterization of a Perfusion Reactor Utilizing Mammalian Cells on Microcarrier BeadsBiotechnology Progress, 16
D. Quick, Michael Shuler (1999)
Use of In Vitro Data for Construction of a Physiologically Based Pharmacokinetic Model for Naphthalene in Rats and Mice To Probe Species DifferencesBiotechnology Progress, 15
Harden McConnell, J. Owicki, J. Parce, D. Miller, G. Baxter, H. Wada, S. Pitchford (1992)
The cytosensor microphysiometer: biological applications of silicon technology.Science, 257 5078
A. Sin, G. Baxter, M. Shuler (2001)
Animal on a chip: a microscale cell culture analog device for evaluating toxicological and pharmacological profiles, 4560
K. Viravaidya, M. Shuler (2008)
Incorporation of 3T3‐L1 Cells To Mimic Bioaccumulation in a Microscale Cell Culture Analog Device for Toxicity StudiesBiotechnology Progress, 20
G. Kelloff, C. Boone, J. Crowell, S. Nayfield, E. Hawk, W. Malone, V. Steele, R. Lubet, C. Sigman (1996)
Risk biomarkers and current strategies for cancer chemopreventionJournal of Cellular Biochemistry, 63
Gregory Baxter, Luc Bousse, T. Dawes, Jeffrey Libby, D. Modlin, J. Owicki, J. Parce (1994)
Microfabrication in silicon microphysiometry.Clinical chemistry, 40 9
L. Gerlowski, Rakesh Jain (1983)
Physiologically based pharmacokinetic modeling: principles and applications.Journal of pharmaceutical sciences, 72 10
K. Viravaidya, A. Sin, M. Shuler (2008)
Development of a Microscale Cell Culture Analog To Probe Naphthalene ToxicityBiotechnology Progress, 20
K. Ikeda, K. Yoshisue, E. Matsushima, S. Nagayama, Kaoru Kobayashi, C. Tyson, K. Chiba, Y. Kawaguchi (2000)
Bioactivation of tegafur to 5-fluorouracil is catalyzed by cytochrome P-450 2A6 in human liver microsomes in vitro.Clinical cancer research : an official journal of the American Association for Cancer Research, 6 11
(1998)
Application of a Novel Packed HurelTM — an in vivo-surrogate assay platform for cell-based studies
(2004)
The design and fabrication of three - chamber microscale cell culture analogue devices with integrated dissolved oxygen sensor
A. Ghanem, M. Shuler (2000)
Combining Cell Culture Analogue Reactor Designs and PBPK Models to Probe Mechanisms of Naphthalene ToxicityBiotechnology Progress, 16
H. Anderson (1985)
Utilization of adipose tissue biopsy in characterizing human halogenated hydrocarbon exposure.Environmental Health Perspectives, 60
Gary Keiloff, C. Boone, J. Crowell, V. Steele, R. Lubet, L. Doody, W. Malone, E. Hawk, C. Sigman (1996)
New agents for cancer chemopreventionJournal of Cellular Biochemistry, 63
M. Shuler, A. Ghanem, D. Quick, M. Wong, P. Miller (1996)
A self-regulating cell culture analog device to mimic animal and human toxicological responses.Biotechnology and bioengineering, 52 1
L. Sweeney, M. Shuler, J. Babish, A. Ghanem (1995)
A cell culture analogue of rodent physiology: Application to naphthalene toxicology.Toxicology in vitro : an international journal published in association with BIBRA, 9 3
A. Sin, Katherine Chin, M. Jamil, Y. Kostov, G. Rao, M. Shuler (2008)
The Design and Fabrication of Three‐Chamber Microscale Cell Culture Analog Devices with Integrated Dissolved Oxygen SensorsBiotechnology Progress, 20
Accurate prediction of the human response to potential pharmaceuticals is difficult, often unreliable, and invariably expensive. Traditional in vitro cell culture assays are of limited value, because they do not accurately mimic the complex environment to which a drug candidate is subjected within the human body. While in vivo animal studies can account for the complex inter-cellular and inter-tissue effects not observable from in vitro assays, animal studies are expensive, labour intensive, time consuming, and unpopular. In addition, there is considerable concern as to whether animal studies can predict human risk sufficiently precisely, because, first, there is no known mechanistic basis for extrapolation from high to low doses, and second, cross-species extrapolation has frequently been found to be problematic with respect to toxicity and pharmacokinetic characteristics. To address these limitations, an interactive, cell-based microfluidic biochip called a Hurel™ was developed. The Hurel system consists of living cells segregated into interconnected “tissue” or “organ” compartments. The organ compartments are connected by a re-circulating culture medium that acts as a “blood surrogate”. The fluidics are designed so that the primary elements of the circulatory system, and more importantly, the interactions of the organ systems, are accurately mimicked. Drug candidates are exposed to a more-realistic animal or human physiological environment, thus providing a higher and more accurate informational content than can the traditional in vitro assays. By affording dynamic assessment of potential toxicity, metabolism, and bioavailability, the device's capabilities hold the potential to markedly improve the prioritisation of drug leads prior to animal studies.
Alternatives to Laboratory Animals – SAGE
Published: Sep 1, 2009
Keywords: cell culture analogue,in vitro,microfluidic biochip,pharmacokinetic modelling
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