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ATLA 20, 575-577, 1992 Around The World- Abstracts of the Main Articles Published in ALTEXVolume 16 (March 1992) and Volume 17 (September 1992) Use of polyclonal antibodies from egg yolk of immunised chickens (minireview). 1 2 1 Max Gassmann & IDrich Hiibscher; Department of Biochemistry, Beckman Center, Stanford University Medical School, Stanford, CA 94305-5307, USA & Institut fur Pharmakologie und Biochemie, Universitat Zurich-Irchel, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland ALTEX 16, 5-12, 1992. Polyclonal antibodies from the egg yolk of immunised chickens are a versatile and sensitive tool for the detection, characterisation, quantitation and localisation of biomolecules. They are widely used for the diagnosis of human and animal diseases. Polyclonal antibodies are typically isolated from the blood of immunised mammals. In contrast, the isolation of chicken antibodies from the egg yolk of immunised chickens is less well-known. In this short review, we outline the method for producing polyclonal egg yolk antibodies and discuss the advantages of this approach with respect to the less invasive treatment of the hens compared to the production of antibodies from mammals. Finally, the potential uses of egg yolk antibodies in basic research, diagnosis, prophylaxis and therapy are presented. Monoclonal antibodies: comparative methods for in vitro
Alternatives to Laboratory Animals – SAGE
Published: Oct 1, 1992
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