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Y. Kuroyanagi (1996)
Design of artificial skin
James Rheinwatd, H. Green (1975)
Seria cultivation of strains of human epidemal keratinocytes: the formation keratinizin colonies from single cell isCell, 6
N. Peppas, A. Khademhosseini (2016)
Make better, safer biomaterialsNature, 540
B. Kinikoglu, J. Rodríguez‐Cabello, O. Damour, V. Hasırcı (2011)
The influence of elastin-like recombinant polymer on the self-renewing potential of a 3D tissue equivalent derived from human lamina propria fibroblasts and oral epithelial cells.Biomaterials, 32 25
(2017)
Human Amniotic Membrane: Basic Science and Clinical Application, 1st edn, 380pp
L. Shahabeddin, François Berthod, Odile Damour, Christian Collombel (1990)
Characterization of skin reconstructed on a chitosan-cross-linked collagen-glycosaminoglycan matrix.Skin pharmacology : the official journal of the Skin Pharmacology Society, 3 2
A. Mantovani, A. Sica, S. Sozzani, P. Allavena, A. Vecchi, M. Locati (2004)
The chemokine system in diverse forms of macrophage activation and polarization.Trends in immunology, 25 12
Parbinder Sahota, J. Burn, N. Brown, S. MacNeil (2004)
Approaches to improve angiogenesis in tissue‐engineered skinWound Repair and Regeneration, 12
C. Cloutier, R. Guignard, G. Bernard, R. Gauvin, D. Larouche, A. Lavoie, D. Lacroix, V. Moulin, L. Germain, F. Auger (2015)
Production of a Bilayered Self-Assembled Skin Substitute Using a Tissue-Engineered Acellular Dermal Matrix.Tissue engineering. Part C, Methods, 21 12
S. Badylak (2007)
The extracellular matrix as a biologic scaffold material.Biomaterials, 28 25
I. Yannas, J. Burke (1980)
Design of an artificial skin. I. Basic design principles.Journal of biomedical materials research, 14 1
Shaoping Zhong, Yanzhong Zhang, C. Lim (2010)
Tissue scaffolds for skin wound healing and dermal reconstruction.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology, 2 5
S. MacNeil (2007)
Progress and opportunities for tissue-engineered skinNature, 445
P. Delvoye, D. Pierard, A. Noel, B. Nusgens, J. Foidart, C. Lapière (1988)
Fibroblasts induce the assembly of the macromolecules of the basement membrane.The Journal of investigative dermatology, 90 3
W. Seet, Manira Maarof, Khairoji Anuar, K. Chua, Abdul Irfan, M. Ng, Bin Aminuddin, B. Ruszymah (2012)
Shelf-Life Evaluation of Bilayered Human Skin Equivalent, MyDerm™PLoS ONE, 7
E. Fuchs (2007)
Scratching the surface of skin developmentNature, 445
C. Auxenfans, A. Thépot, V. Justin, A. Hautefeuille, L. Shahabeddin, O. Damour, P. Hainaut (2009)
Characterisation of human fibroblasts as keratinocyte feeder layer using p63 isoforms status.Bio-medical materials and engineering, 19 4-5
J. Exbrayat (2013)
Histochemical and cytochemical methods of visualization
Sayani Chattopadhyay, R. Raines (2014)
Collagen‐based biomaterials for wound healingBiopolymers, 101
Naeema Mayet, Y. Choonara, Pradeep Kumar, L. Tomar, Charu Tyagi, L. Toit, V. Pillay (2014)
A comprehensive review of advanced biopolymeric wound healing systems.Journal of pharmaceutical sciences, 103 8
C. Auxenfans, J. Fradette, C. Lequeux, L. Germain, B. Kinikoglu, N. Béchetoille, F. Braye, F. Auger, O. Damour (2009)
Evolution of three dimensional skin equivalent models reconstructed in vitro by tissue engineering.European journal of dermatology : EJD, 19 2
S. Priya, H. Jungvid, Ashok Kumar (2008)
Skin tissue engineering for tissue repair and regeneration.Tissue engineering. Part B, Reviews, 14 1
J. Rheinwald, H. Green (1975)
Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells.Cell, 6 3
R. Lanza, R. Langer, J. Vacanti (2014)
Principles of tissue engineering
S. MacNeil (2008)
Biomaterials for tissue engineering of skinMaterials Today, 11
M. Climov, Erika Medeiros, E. Farkash, Jizeng Qiao, C. Rousseau, Shumin Dong, Agatha Zawadzka, Waldemar Racki, Ahmad Al-Musa, D. Sachs, M. Randolph, Christene Huang, T. Bollenbach (2016)
Bioengineered Self-assembled Skin as an Alternative to Skin GraftsPlastic and Reconstructive Surgery Global Open, 4
E. Pashuck, M. Stevens (2012)
Designing Regenerative Biomaterial Therapies for the ClinicScience Translational Medicine, 4
B. Kinikoglu, C. Auxenfans, P. Pierrillas, V. Justin, P. Breton, C. Burillon, V. Hasırcı, O. Damour (2009)
Reconstruction of a full-thickness collagen-based human oral mucosal equivalent.Biomaterials, 30 32
F. Groeber, M. Holeiter, M. Hampel, Svenja Hinderer, K. Schenke-Layland (2011)
Skin tissue engineering--in vivo and in vitro applications.Advanced drug delivery reviews, 63 4-5
B. Kinikoglu, M. Rovere, M. Haftek, V. Hasırcı, O. Damour (2012)
Influence of the mesenchymal cell source on oral epithelial developmentJournal of Tissue Engineering and Regenerative Medicine, 6
Tissue engineered full-thickness human skin substitutes have various applications in the clinic and in the laboratory, such as in the treatment of burns or deep skin defects, and as reconstructed human skin models in the safety testing of drugs and cosmetics and in the fundamental study of skin biology and pathology. So far, different approaches have been proposed for the generation of reconstructed skin, each with its own advantages and disadvantages. Here, the classic tissue engineering approach, based on cell-seeded polymeric scaffolds, is compared with the less-studied cell self-assembly approach, where the cells are coaxed to synthesise their own extracellular matrix (ECM). The resulting full-thickness human skin substitutes were analysed by means of histological and immunohistochemical analyses. It was found that both the scaffold-free and the scaffold-based skin equivalents successfully mimicked the functionality and morphology of native skin, with complete epidermal differentiation (as determined by the expression of filaggrin), the presence of a continuous basement membrane expressing collagen VII, and new ECM deposition by dermal fibroblasts. On the other hand, the scaffold-free model had a thicker epidermis and a significantly higher number of Ki67-positive proliferative cells, indicating a higher capacity for self-renewal, as compared to the scaffold-based model.
Alternatives to Laboratory Animals – SAGE
Published: Dec 1, 2017
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