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TOBACCO FLEA BEETLE MORTALITY FOLLOWING EXPOSURE TO IMIDACLOPRID TREATED LEAVES, 2012

TOBACCO FLEA BEETLE MORTALITY FOLLOWING EXPOSURE TO IMIDACLOPRID TREATED LEAVES, 2012 Arthropod Management Tests 2013, Vol. 38 doi: 10.4182/amt.2013.F75 (F75) TOBACCO: Nicotiana tobacum, ‘NC196’ TOBACCO FLEA BEETLE MORTALITY FOLLOWING EXPOSURE TO IMIDACLOPRID TREATED LEAVES, 2012 Hannah J. Burrack Department of Entomology North Carolina State University Campus Box 7630 Raleigh, NC 27695 Phone: 919-513-4344 Fax: 919-515-3748 E-mail: hannah_burrack@ncsu.edu Tobacco flea beetle (TFB): Epitrix hirtipennis (Melsheimer) As part of an ongoing project to monitor the potential for resistance development in TFB, we collected live, active TFB adults in a commercial tobacco field in Johnston County, NC that had been treated in the greenhouse with 0.9 fl oz per 1000 plants of a 2F imidacloprid formulation. TFB were transported to the laboratory and exposed to 60 mm tobacco leaf discs that had either been: dipped in a solution equivalent to the lowest foliar rate of Admire Pro (0.7 fl oz/acre); dipped in a solution equivalent to the highest foliar rate of Admire Pro (1.4 fl oz/acre); collected from a field grown plants that had been treated with 1.2 fl oz/ Admire Pro 1000 plants in the greenhouse 5 week prior; or excised from completely untreated leaves (UTC). Simulated foliar treatments were diluted in 1000 ml water, based on a 30 GPA application volume, and leaves were allowed to dry before TFB exposure. Greenhouse treated leaves and UTC leaves were used as is. Leaves were placed in 60 mm diameter Petri dishes lined with slightly damp filter paper. Ten (10) TFB adults were placed in each dish, and each treatment was replicated 6 to 10 times. Arenas were randomly arranged on a laboratory bench and held at 25°C and 12:12, L:D. Survivorship was assessed at 24 and 72 h after exposure, and the number of TFB feeding holes per leaf disc was counted at the end of the experiment. Data were analyzed via ANOVA using Proc Mixed (SAS v. 9.2.1; Cary, NC) with replicate as a random variable and treatment as a fixed variable. Means were separated via Fisher’s Protected LSD (α = 0.05). Both the lowest and highest labeled foliar rates, applied as leaf dip treatments, results in high TFB mortality and reduced feeding activity. Mortality on leaves collected from field grown plants that were treated in the greenhouse 5 weeks prior to exposure was similar to that on UTC leaves. This suggests that while TFBs are sensitive to imidacloprid, insufficient insecticide was present in greenhouse treated leaves to cause mortality. Future activity will address the longevity of greenhouse applications against TFB. Table 1. Percentage of live TFB Flea beetle holes, Treatment 12h 72h 72 h 0.7 fl oz Admire Pro/acre leaf dip 38.25a 1.67a 0.83a 1.4 fl oz Admire Pro/acre leaf dip 41.26a 4.44a 0.44a 1.2 fl oz Admire Pro/1000 plants greenhouse treatment 88.00b 75.25b 27.90b UTC 100.00b 79.50b 32.00b Values within the same column followed by the same letter are not significantly different (α = 0.05). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Arthropod Management Tests Oxford University Press

TOBACCO FLEA BEETLE MORTALITY FOLLOWING EXPOSURE TO IMIDACLOPRID TREATED LEAVES, 2012

Burrack, Hannah J.
Arthropod Management Tests , Volume 38 (1) – Jan 1, 2013
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Oxford University Press
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2155-9856
DOI
10.4182/amt.2013.F75
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Abstract

Arthropod Management Tests 2013, Vol. 38 doi: 10.4182/amt.2013.F75 (F75) TOBACCO: Nicotiana tobacum, ‘NC196’ TOBACCO FLEA BEETLE MORTALITY FOLLOWING EXPOSURE TO IMIDACLOPRID TREATED LEAVES, 2012 Hannah J. Burrack Department of Entomology North Carolina State University Campus Box 7630 Raleigh, NC 27695 Phone: 919-513-4344 Fax: 919-515-3748 E-mail: hannah_burrack@ncsu.edu Tobacco flea beetle (TFB): Epitrix hirtipennis (Melsheimer) As part of an ongoing project to monitor the potential for resistance development in TFB, we collected live, active TFB adults in a commercial tobacco field in Johnston County, NC that had been treated in the greenhouse with 0.9 fl oz per 1000 plants of a 2F imidacloprid formulation. TFB were transported to the laboratory and exposed to 60 mm tobacco leaf discs that had either been: dipped in a solution equivalent to the lowest foliar rate of Admire Pro (0.7 fl oz/acre); dipped in a solution equivalent to the highest foliar rate of Admire Pro (1.4 fl oz/acre); collected from a field grown plants that had been treated with 1.2 fl oz/ Admire Pro 1000 plants in the greenhouse 5 week prior; or excised from completely untreated leaves (UTC). Simulated foliar treatments were diluted in 1000 ml water, based on a 30 GPA application volume, and leaves were allowed to dry before TFB exposure. Greenhouse treated leaves and UTC leaves were used as is. Leaves were placed in 60 mm diameter Petri dishes lined with slightly damp filter paper. Ten (10) TFB adults were placed in each dish, and each treatment was replicated 6 to 10 times. Arenas were randomly arranged on a laboratory bench and held at 25°C and 12:12, L:D. Survivorship was assessed at 24 and 72 h after exposure, and the number of TFB feeding holes per leaf disc was counted at the end of the experiment. Data were analyzed via ANOVA using Proc Mixed (SAS v. 9.2.1; Cary, NC) with replicate as a random variable and treatment as a fixed variable. Means were separated via Fisher’s Protected LSD (α = 0.05). Both the lowest and highest labeled foliar rates, applied as leaf dip treatments, results in high TFB mortality and reduced feeding activity. Mortality on leaves collected from field grown plants that were treated in the greenhouse 5 weeks prior to exposure was similar to that on UTC leaves. This suggests that while TFBs are sensitive to imidacloprid, insufficient insecticide was present in greenhouse treated leaves to cause mortality. Future activity will address the longevity of greenhouse applications against TFB. Table 1. Percentage of live TFB Flea beetle holes, Treatment 12h 72h 72 h 0.7 fl oz Admire Pro/acre leaf dip 38.25a 1.67a 0.83a 1.4 fl oz Admire Pro/acre leaf dip 41.26a 4.44a 0.44a 1.2 fl oz Admire Pro/1000 plants greenhouse treatment 88.00b 75.25b 27.90b UTC 100.00b 79.50b 32.00b Values within the same column followed by the same letter are not significantly different (α = 0.05).

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Arthropod Management Tests – Oxford University Press

Published: Jan 1, 2013

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