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The influence of particle size of Enogen Feed corn and conventional yellow dent corn on nursery and finishing pig performance, carcass characteristics and stomach morphology

The influence of particle size of Enogen Feed corn and conventional yellow dent corn on nursery... Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 The influence of particle size of Enogen Feed corn and conventional yellow dent corn on nursery and finishing pig performance, carcass characteristics and stomach morphology Hadley R. Williams *, Mike D. Tokach*, Jason C. Woodworth*, Joel M. DeRouchey*, Robert D. Goodband*, Jordan T. Gebhardt†, and Chad B. Paulk‡ *Department of Animal Sciences and Industry, College of Agriculture, Kansas State University, Manhattan, KS 66506 USA †Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506 USA ‡Department of Grain Science and Industry, College of Agriculture, Kansas State University, Manhattan, KS 66506 USA 1 Corresponding author: hadley1@ksu.edu © The Author(s) 2021. Published by Oxford University Press on behalf of the American Society of Animal Science. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 ABSTRACT: Enogen Feed corn is a variety developed by Syngenta Seeds (Downers Grove, IL) that has been genetically modified to contain an α-amylase enzyme trait (SYT-EFC). Originally, Enogen feed corn was developed for the ethanol industry due to its reduction in viscosity of the corn mash, thus eliminating the need to add a liquid form of the α-amylase enzyme. However, there is potential application for Enogen Feed corn to be used in livestock diets due to the increase in α-amylase enzyme potential to increase starch digestibility. A more common method of increasing starch digestibility in corn is to finely grind it to reduce particle size. This increases the surface area and allows for greater interaction with digestive enzymes. We hypothesized that pigs fed Enogen feed corn potentially could achieve similar gain:feed ratio (G:F) at larger particle sizes than conventional corn because of the differences in starch digestibility. In Exp. 1, a total of 360 pigs (DNA 200 × 400, Columbus, NE; initially 6.6 ± 0.1 kg BW) were used with 5 pigs per pen and 12 pens per treatment. Treatments were arranged in a 2 × 3 factorial with main effects of corn source (Enogen Feed corn or conventional yellow dent corn) and ground corn particle size (300, 600, or 900 µm). Overall, there was a corn source × particle size interaction (linear, P = 0.027) for G:F. There was no effect due to particle size when pigs were fed conventional yellow dent corn, but in pigs fed Enogen Feed corn, G:F increased with decreasing particle size. Neither corn source nor particle size affected (P > 0.05) overall average daily gain (ADG) or average daily feed intake (ADFI). In Exp. 2, a total of 323 pigs (241  600; DNA, Columbus, NE; initially 50.0 ± 1.3 kg) were used with 9 pigs per pen and 6 pens per treatment. Treatments were identical as Exp. 1. Overall, corn source had no effect on finishing pig ADG, ADFI or G:F. For corn particle size, ADG and G:F increased (linear, P < 0.014) and ADFI decreased (P = 0.043) as particle size decreased. For stomach morphology, there was a tendency for a corn source × particle size interaction (P = 0.055) for Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 keratinization score with keratinization increasing linearly (P = 0.001) as particle size of the corn decreased for yellow dent corn with no change in keratinization score as particle size decreased for Enogen Feed corn. In summary, reducing corn particle size improved G:F with no major differences observed between corn sources for overall pig performance. Key words: α-amylase, Enogen Feed corn, finishing pigs, growth performance, nursery pigs, particle size Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 INTRODUCTION Cereal grains such as corn, grain sorghum, wheat, and barley typically provide most of the dietary energy in swine diets. Due to the elevated energy density and availability when compared to other cereal grains, corn is the most commonly used cereal grain among pork production systems in the United States. Corn typically contains 65% starch (NRC, 2012) with apparent total tract digestibility around 90 to 96% (Rojas and Stein, 2015). Genetic modification of corn varieties has enhanced nutrient profiles. Genetically modified corn varieties such as NutriDense and low-phytate corn, have been previously evaluated in growing-finishing pigs (Spencer et al., 2000, Hastad et al., 2005). Enogen Feed corn, (Syngenta Seeds, Downers Grove, IL), has been genetically enhanced to contain a thermotolerant α-amylase enzyme trait. While Enogen Feed corn was originally intended to be used by the ethanol industry, the increase in α-amylase may have application in livestock feeding. Recent trial conducted by Ochonski et al. (2019) determined that utilizing Enogen Feed corn in finishing pig diets tended to increase average daily gain compared to pigs that were fed diets with conventional corn, but there was no influence on feed efficiency. Although corn varieties can influence growth performance, reducing its particle size can influence performance as well. Grinding corn to reduce particle size increases the surface area, allowing more enzymatic activity to occur and increase the digestibility of both energy and other nutrients (Kim et al., 2002). Research in finishing pigs has shown that reducing corn particle size from 1,000 to 400 µm improves feed efficiency (Wondra et al., 1995). However, decreasing particle size below 700 µm is not always beneficial. Wondra et al. (1995) suggested reducing particle size causes more lesions and greater keratinization of the esophageal region due to the lack of protective mucus in this region of the stomach. We Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 hypothesized that feeding coarse Enogen Feed corn will have the same response as feeding fine particle size conventional corn due to the higher availability of starch. Therefore, the objective of this study was to determine the effects of feeding Enogen Feed corn compared to conventional yellow dent corn at different particle sizes on nursery and finishing pig growth performance, carcass characteristics and stomach morphology. MATERIALS AND METHODS The Kansas State University and Institutional Animal Care and Use Committee approved the protocols used in these experiments. Ingredients and chemical analysis All diets for experiment 1 and 2 were manufactured at the Kansas State University O.H. Kruse Feed Technology Innovation Center (Manhattan, KS). All corn in this study was source from a single source for each corn type. Conventional U.S. No. 2 dent corn and Enogen Feed corn were ground to approximately 300, 600 or 900 µm using a roller mill (Model 924, RMS Roller Grinder, Harrisburg, SD) equipped with 3 roll pairs. The top and middle roll pairs had a speed differential of 1.5 with the front rolls operating at 1,285 rpm and the back rolls at 850 rpm. The bottom roll pair differential was 0.68 with the front roll operating at 1,020 rpm and the back roll at 1,500 rpm. The top, middle, and bottom roll pairs had 2.36 and 2.36 corrugations/cm, 4.72 and 5.51 corrugations/cm, and 6.30 and 7.09 corrugations/cm, respectively. Adjustments were made to the roll gap setting to achieve the desired particle sizes. Samples of ground corn were obtained during each feed manufacturing event for each experiment and analyzed (Table 1; Ward Laboratories, Inc., Kearney, NE) for dry matter (method 935.29; AOAC Inc., Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 2019), starch (Application #322. 2000), crude protein (method 990.03; AOAC Inc., 2019), ether extract (ANKOM Technology, 2004), acid detergent fiber and neutral detergent fiber (ANKOM Technology, 2005), calcium (method 6.3; Kovar, 2003) and phosphorus (method 6.3; Kovar, 2003). Particle size analysis was conducted on ground corn samples (100 g) in duplicate according to the ANSI/ASAE S319.2 (1995) standard method. Samples were analyzed both with (5 g) and without dispersing agent (Gilson Company, Inc., Lewis Center, OH) using 2 separate stainless-steel sieve stacks (13 sieves each) to prevent residual dispersing agent from affecting results. Both sieve stacks contained agitators and were placed in the Ro-Tap (Model RX-29, W. S. Tyler Industrial Group, Mentor, OH) machine for 15 min, using the same sieve and agitator configurations as Gebhardt et al. (2018). Representative diet samples from each manufacturing event were obtained from each treatment within experiment and stored at -20C until analysis. Diet samples were analyzed (Ward Laboratories, Inc., Kearney, NE) for dry matter, crude protein, acid detergent fiber, neutral detergent fiber, Ca, and P using the same procedures as used for ground grain samples. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Animals and diets In both experiments, treatments were arranged in a 2  3 factorial with main effects of corn source (conventional yellow dent corn or Enogen Feed corn) and ground corn particle size (300, 600 or 900 µm). Diets were similar between all treatments, and Enogen Feed corn replaced conventional yellow dent corn on an equal weight basis (Tables 2 and 3). Experiment 1 was conducted at the Kansas State University Segregated Early Weaning facility (Manhattan, KS). A total of 360 barrows (DNA 200 × 400, Columbus, NE; initially 6.6 ± 0.01 kg) were placed in pens with 5 pigs per pen. Pens (1.22  1.22 m) had metal slatted floors and were equipped with a four-hole stainless steel dry feeder and a water cup. Pigs were weaned at approximately 21 d of age and placed in pens based on initial body weight (BW), and fed a common pelleted started diet for approximately 7-d. On d 7, which was considered d 0 of the study, pens of pigs were allotted to 1 of 6 dietary treatments in a randomized complete block design with BW as the blocking factor. There were 12 replications (pens) per treatment. Diets were provided ad libitum in mash form and split into 2 separate phases. Phase 1 was fed from d 0 to 14 and phase 2 was fed from d 14 to 35. Pens of pigs were weighed, and feed disappearance was measured weekly to calculate average daily gain (ADG), average daily feed intake (ADFI), and gain to feed ratio (G:F). Experiment 2 was conducted at the Kansas State University Swine Teaching and Research Center (Manhattan, KS). A total of 323 pigs (241  600; DNA, Columbus, NE; initially 50.0 ± 1.3 kg) were used in an 83-d trial. Pens of pigs were allotted to 1 of 6 dietary treatments in a randomized complete block design with BW as the blocking factor with 9 pigs per pen and 6 pens per treatment. Diets were fed in mash form in 3 separate phases. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 The facility was totally enclosed and environmentally regulated, containing 36 pens. Each pen (3.00 × 2.44 m) was equipped with a 1-cup waterer and a dry, single-sided feeder (Farmweld, Teutopolis, IL) with two feeder spaces. Pens were located over a completely slatted concrete floor with a 1.22-m deep pit underneath for manure storage. A robotic feeding system (FeedPro; Feedlogic Corp., Wilmar, MN) was used to deliver and record daily feed additions to each pen. Pens were equipped with adjustable gates to allow space allowance per pig to be maintained if a pig died or was removed during the experiment. Growth performance was assessed by recording BW and feed disappearance every 2 weeks and at the conclusion of the study (d 83). On d-79, 2 pigs per pen, 1 barrow and 1 gilt of equal weight based on average of the pen weight, were selected and transported to a USDA inspected packing facility (Natural Food Holdings, Sioux Center, IA) for slaughter and to collect stomachs. The stomachs were taken to the Kansas State University Veterinary Diagnostic Laboratory where a scoring system was used to determine the severity of ulceration and keratinization of the esophageal opening of the stomach (DeJong, 2016). Stomach ulceration score was based on a scale of 1 to 4, with 1 = no ulceration, 2 = < 25% ulceration, 3 = 25-75% ulceration and 4 = > 75% ulceration. This scoring criteria was the same for keratinization. On d 83, the remaining pigs were individually tattooed with a unique ID number, and a radio frequency identification (RFID) transponder was inserted into the right ear to allow carcass measurements to be recorded on an individual pig basis. Final individual pig weights were taken, and pigs were transported approximately 2.5 h to a commercial packing plant (Triumph Foods, St. Joseph, MO) and held in lairage for approximately 7 h before slaughter. At the plant, hot carcass weight (HCW) was determined immediately after evisceration. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Backfat and loin depth were measured with an optical probe (Fat-O-Meter, SFK, Herlev, Denamrk) inserted between the third and fourth rib (counting from the ham end of the carcass) at a distance approximately 7 cm from the dorsal midline. Percentage lean was calculated using proprietary equations from the packing plant. Carcass yield was calculated by dividing the individual HCW obtained from the packing plant by the individual final live weight obtained at the farm. Statistical Analysis Treatments were analyzed as a randomized complete block design for two-way ANOVA using the lmer function from the lme4 package in R (version 3.5.1 (2018-07-.2)) with pen considered the experimental unit, average pen body weight at allotment to treatment diets as blocking factor included in the model as a random effect, and treatment as fixed effect. The main effects of corn source and particle size, as well as their interactions, were tested. Differences between treatments were considered significant at P ≤ 0.05, and a tendency for significance at 0.05 < P ≤ 0.10. RESULTS Chemical and particle size analysis The chemical analysis for ground corn was similar across sources for both Exp. 1 and 2 (Table 1). Samples analyzed with dispersing agent resulted in lower geometric mean diameter (dgw) and greater geometric standard deviation (sgw) than those analyzed without agent as expected (Table 4 and 5). Kalivoda et al. (2017) observed similar findings when comparing methods and suggested that the inclusion of a dispersing agent best facilitated the movement of fine material through sieves and reduced possible Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 agglomerations due to forces such as static charge. Thus, particle size targets and roller mill settings for both trials were based on the analysis with the inclusion of dispersing agent. For Exp. 1, the target particle sizes were achieved and remained similar when comparing conventional to Enogen feed corn (Table 4). For Exp. 2, the particle size targets were again achieved and remained similar across corn source with the exception of a lower than expected dgw in phase 1 for the highest particle size for Enogen feed corn (785 µm) when compared to the closer to target conventional corn (911 µm; Table 5). Complete diet nutrient analysis matched expected values for Exp. 1 and 2 (Table 6 and 7). Experiment 1 Interactive and main effects are reported in Tables 8 and 9. During phase 1 (d 0 to 14), there was no evidence for interaction or main effect differences for ADG and ADFI for ground corn particle size. However, there was a corn source × particle size interaction (linear, P = 0.027) for G:F. There were no changes in G:F for pigs fed decreasing particle size of conventional yellow dent corn, but a linear improvement in G:F was observed as particle size decreased for pigs fed Enogen Feed corn (P < 0.001). During phase 2 (d 14 to 35), there was no evidence for differences in ADG among pigs fed either corn source or different particle sizes. There was a tendency for a corn source × particle size interaction (quadratic, P = 0.071) for ADFI, with no differences in ADFI for pigs fed conventional yellow dent corn as particle size changed (P = 0.540), but an increase then decrease in ADFI as particle size increased in pigs fed Enogen Feed corn. There was also a tendency for a corn source × particle size interaction (linear, P = 0.095) for G:F. As particle size of conventional yellow dent corn was reduced from 900 to 600 micron we observed an improvement in G:F and Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 then decreased as the particle size was reduced from 600 to 300 micron. For pigs fed Enogen Feed corn, as particle size was reduced from 900 to 300 micron G:F was improved. There was no difference among pigs fed conventional yellow dent corn, but G:F improved as particle size decreased in pigs fed Enogen Feed corn. For overall performance (d 0 to 35), there was no evidence for differences in ADG due to corn source or particle size. There was a tendency for a corn source × particle size interaction (quadratic, P = 0.086) for ADFI. There was no change in ADFI in pigs fed conventional yellow dent corn regardless of corn particle size, whereas pigs fed Enogen Feed corn had an increase then decrease in ADFI as particle size increased. For overall G:F, there was also a corn source × particle size interaction (linear, P = 0.027). There was no difference in G:F when pigs were fed conventional yellow dent corn regardless of corn particle size, but for pigs fed Enogen Feed corn, G:F improved with decreasing particle size. Experiment 2 There was no evidence for a corn source × particle size interaction for any growth response criteria (Table 10). There was no evidence for difference in growth performance or carcass characteristics between corn sources, except for a tendency for greater (P = 0.064) ADFI from d 56 to 83 of the experiment for pigs fed Enogen Feed corn compared with pigs fed conventional yellow dent corn. For BW, there was a tendency for greater (P < 0.10) BW on d 0 and 28 for pigs fed conventional yellow dent corn compared to pigs fed Enogen Feed corn. This small initial difference disappeared by d 56 with no differences in BW between pigs fed either corn source for the remainder of the experiment. As expected, G:F improved (linear, P < 0.05) during each phase and overall as corn particle size decreased. Overall, ADFI decreased (linear, P = 0.043) as corn particle size decreased (Table 11) while ADG increased Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 (linear, P = 0.014) as particle size decreased. The improvement in ADG led to a tendency for increased (linear, P = 0.056) BW on d 83 as corn particle decreased. For carcass characteristics, there was a tendency for an increase (linear, P = 0.093) in HCW as corn particle size decreased in the diet. Carcass yield also increased (linear, P = 0.023) as corn particle size decreased. For stomach morphology (Table 12), there was a tendency for a corn source × particle size interaction (P = 0.055) for keratinization score. This was caused by a lower incidence of keratinization in stomachs of pigs fed Enogen Feed corn when ground to 300 or 900 µm than pigs fed conventional yellow dent corn at these particle sizes with similar keratinization score when both corn sources were ground to 600 µm. Neither corn source nor particle size affected stomach ulceration. DISCUSSION The United States is the world’s largest producer of corn, with an annual production of approximately 13 billion bushels in 2019 (NCGA, 2020). Corn in the United States is generally grown for 3 major uses: food production, livestock use, and the ethanol industry. A new corn hybrid, developed by Syngenta Seeds, Enogen Feed corn, was originally developed with the intent to be used in the ethanol industry. Enogen Feed corn has the ability to reduce the viscosity of its corn mash, thus eliminating the need to add a liquid form of the α-amylase enzyme. The chemical analysis of ground corn and complete experimental diets found an increase in starch concentration for Exp.1 for ground Enogen Feed corn and an increase in neutral detergent fiber (NDF) in both the Enogen Feed corn ground corn sample and complete experimental diets. Neutral detergent fiber is the general class of polysaccharides Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 that includes most pentosans but do not include certain soluble fibers such as arabino-xylan. Neutral detergent fibers are resistant to digestion by endogenous enzymes and are slowly but incompletely fermented to volatile fatty acids in the large intestine. An increase in dietary NDF decreases energy availability, causing increased feed intake to meet the nutrient requirements for the pig (Sauber and Owens, 2000). Starch is the major storage carbohydrate of cereal grains. Corn being the most prevalent of cereal grains has a starch content of 65% (NRC, 2012). The digestion of starch is first initiated when feed interacts with salivary amylase (Englyst and Hudson, 2000). Digestion by salivary amylase is short, due to the decrease of pH in the stomach as feed is ingested. Most of the starch digestion takes place in the small intestine, where the starch molecule is hydrolyzed into maltose, maltotriose, and isomaltose subunits by pancreatic α- amylase and isomaltase enzymes (Groff and Gropper, 2000). The digestion of starch is an efficient process, with approximately 95% of starch digestion occurring in the small intestine (Rojas and Stein, 2016). Enogen Feed corn contains an α-amylase enzyme trait (SYT-EFC). The SYT-EFC α-amylase enzyme is thought to help with the starch degradation and improve the digestibility of corn by converting starch into fermentable sugars. Although there is limited research on starch digestion of Enogen Feed corn in monogastrics, a study by Jolly- Breithaupt et al. (2016) observed when finishing cattle were feed corn with the SYT-EFC α- amylase enzyme, there was a numerical increase in post-ruminal starch digestibility compared to cattle fed conventional yellow dent corn. One of the main drivers of improved feed efficiency is an increase in the ability to utilize energy in the diet (Patience et al., 2016). With the increase of α-amylase in Enogen Feed corn, starch can potentially be converted to sugar more readily, allowing more energy Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 for the animal which in turn should improve feed efficiency. Ochonski et al. (2019) evaluated the use of Enogen Feed corn fed to finishing pigs. While they did not observe any difference in G:F, they observed a numerical increase in ADG when pigs were fed Enogen Feed corn compared to conventional yellow dent corn. In our study, we did not observe any significant differences for ADG, ADFI or G:F when comparing conventional yellow dent corn and Enogen Feed corn on overall finishing pig performance. Feed processing of cereal grains also can influence feed efficiency. The main reason cereal grains, like corn, are ground to fine particle sizes is to increase particle surface area and energy utilization (Wondra et al., 1995). A study conducted by Rojas and Stein (2015) evaluated the apparent ileal digestibility (AID) of starch and confirmed that decreased corn particle size from 865 to 339 µm linearly increased AID of starch from 89.0% to 96.6%. This is likely due to increased access to the starch granules for α-amylase, which increases starch digestibility in the small intestine. As digestibility increases, feed intake may also decrease because less feed is needed to meet the animal’s nutrient requirements. Although not statistically significant, in both experiments, we observed a numerical decrease in feed intake as corn particle size decreased. For nursery pigs in Exp. 1, as corn particle size was reduced from 900 to 300 µm for Enogen Feed corn, G:F improved linearly. Similarly, Bokelman et al. (2014) also observed improved G:F as diet particle size was reduced. However, a study conducted by DeJong et al. (2014) observed no improvement in G:F when the particle size of corn was reduced from approximately 900 µm to approximately 325 µm for nursery pigs. The reasons DeJong et al. (2014) observed no improvement in G:F was due to decreased ADG and ADFI when the corn was ground to very fine particle sizes. In our study we observed a tendency for a quadratic Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 effect in ADG, with pigs fed 300 and 600 µm corn having increased gain compared to the 900 µm, resulting in a linear improvement in G:F as corn particle size was decreased. When a pig is weaned, their enzyme activity is relatively low for enzymes like amylase and protease. Amylase is an endogenous enzyme that originates from saliva and brush border enzymes and is used to break down starch in the small intestine. In our study, we observed an improvement in ADG and G:F when pigs were fed Enogen Feed corn compared with pigs fed conventional yellow dent corn the 14 days post weaning. The increase in gain with pigs fed Enogen Feed corn could have been related to the increase in α-amylase in the corn. A study conducted by Yi et al., (2013) found an increase in weight gain when supplementing an enzyme complex of amylase, protease and xylanase to wean pigs. In our study, the improvement in gain, however, was not maintained during the subsequent period from d 14 to35 after weaning. Due to a large volume of feed consumed by finishing pigs over their life span, a small improvement in feed efficiency can result in large economic benefits. A study conducted by DeJong et al. (2013) with finishing pigs observed an improvement in G:F when reducing corn particle size from 596 to 320 µm. Other studies have shown that reducing the particle size of corn in the diet can decrease ADFI when fed to finishing pigs (DeJong et al., 2013, Nemechek et al., 2016). In Exp. 2, reducing corn particle size from 900 to 300 µm resulted in an increase in ADG, decrease in ADFI, and improvement in G:F. In the current study, a 1% improvement of G:F for every 100 µm decrease of ground corn was observed, which is consistent to the findings by Wondra et al. (1995) who saw an 8% improvement as corn particle size was reduced from 1,000 to 400 µm. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 While reducing corn particle size for finishing pigs has been shown to improve G:F, grinding corn to a fine particle has been shown to cause gastric lesions (Maxwell et al., 1970). In Exp. 2, there was no evidence for difference in corn source or particle size on ulcer score. However, pigs fed Enogen Feed corn had decreased keratinization score compared to pigs fed conventional yellow dent corn. As corn particle size was decreased from 900 to 300 µm, keratinization score increased for pigs fed conventional yellow dent corn. Keratinization, the process of keratin forming around the esophageal opening of the stomach, occurs prior to ulceration. Once the keratinized tissue is sloughed off, ulcerations are visible. Ulceration occurs with lower particle size due to reduced viscosity of the feed form in the stomach lowering the pH in the stomach. This difference in keratinization scores between corn sources warrants further investigation. In summary, Enogen Feed corn can be effectively used in nursery or finishing pig diets and result in less keratinization on stomach tissue than pigs fed conventional yellow dent corn. This data also confirms previous research that observed reducing particle size of corn improves G:F in both nursery and finishing pig diets. However, feeding Enogen Feed corn ground to coarser particle size did not yield the same response for G:F as conventional yellow dent corn at finer diet particle sizes as had been hypothesized. 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Kovar, J. L. 2003. Method 6.3 Inductively coupled plasma spectroscopy. University of Wisconsin-Extension, Madison, WI. Maxwell, C. V., E. M. Reimann, W.G. Hoekstra, T. Kowalcyzyk, N. J. Benevenga, and R. H. Grummer. 1970. Effect of dietary particle size on lesion development and on the contents of various regions of the swine stomach. J. Anim. Sci. 30-911-922. doi:10.2527/jas1970.306911x. National Corn Growers Association. 2019. Animal Ag facts. Date accessed: Aug. 17, 2020. Retrieved from: https://dt176nijwh14e.cloudfront.net/file/164/DYK-Animal%20Ag- 1.pdf. Nemechek, J. E., M. D. Tokach, S. S. Dritz, R. D. Goodband, J. M. DeRouchey, and J. C. Woodworth. 2016. Effects of diet form and corn particle size on growth performance and carcass characteristics of finishing pigs. Anim. Feed Sci. Technol. 214:136-141. doi:10.1016/j.anifeedsci.2016.02.002. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 th NRC. 2012. Nutrient Requirements of swine. 11 ed. Natl. Acad. Press, Washington, DC. doi:10.17226/13298. Ochonski, P., F. Wu, E. Arkfeld., J. M. Lattimer, J. M. DeRouchey, S. S. Dritz, R. D. Goodband, J. C. Woodworth, and M. D. Tokach. 2019. Evaluation of high amylase corn on growth performance and carcass characteristics of finishing pigs. Kansas Agricultural Experiment Station Research Reports: Vol. 5: Iss. 8. doi:10.4148/2378-5977.7850. Patience, J. F., M. C. Rossoni-Serao, and N. A. Gutierrez. 2016. A review of feed efficiency in swine: biology and application. J. Anim. Sci. Biotechn. 6:33. doi:10.1186/s40104-015- 0031-2. Rojas, O. J. and H. H. Stein. 2015. Effects of reducing the particle size of corn grain on the concentration of digestible and metabolizable energy and on the digestibility of energy and nutrients in corn grain fed to growing pigs. Livest Sci. 181:187-193. doi:10.1016/j.livsci.2015.09.013. Sauber, T. E. and F. N. Owens. 2000. Cereal Grains and By-Products for Swine. In: A. J. Lewis nd and L. L. Southern, editors, Swine Nutrition. 2 ed. CRC Press, Boca Raton, FL. p. 799- Spencer, J. D., G. L. Allee, and T. E. Sauber. 2000 Growing-finishing performance and carcass characteristics of pigs fed normal and genetically modified low-phytate corn. J. Anim. Sci. 78:1529-1536. doi:10.2527/2000.7861529x. Wondra, K. J., J. D. Hancock, K. C. Behnke, R. H. Hines, and C. R. Stark. 1995. Effects of particle size and pelleting on growth performance, nutrient digestibility, and Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 stomach morphology in finishing pigs. J. Anim. Sci. 73, 757-763. doi:10.2527/1995.733757x. Yi, J. Q., X. S. Piao, Z. C. Li, H. Y. Zhang, Y. Chen, Q. Y. Li, J. D. Liu, Q. Zhang, Y. J. Run, and B. Dong. 2013. The effects of enzyme complex on performance, intestinal health and nutrient digestibility of weaned pigs. Asian-Australas. J. Anim. Sci., 26, 1181-1188. doi:10.5713/ajas.2013.13129. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Table 1. Chemical analysis of corn varieties of Exp. 1 and 2 (as-fed basis) Experiment 1 Experiment 2 Conventional Enogen Feed Enogen Feed 2 3 Item, % corn Conventional corn Dry matter 87.38 87.73 87.72 87.80 Starch 59.68 64.15 60.60 59.76 Crude protein 7.74 7.45 7.68 7.40 Ether extract 4.10 4.10 3.88 4.16 Acid detergent fiber 1.70 1.91 1.55 1.73 Neutral detergent fiber 6.32 7.31 6.20 7.22 Ca 0.10 0.13 0.10 0.11 P 0.23 0.21 0.22 0.21 Corn samples were collected at time of feed manufacturing and pooled for analysis (Ward Laboratories, Inc., Kearney, NE). Each value represents the mean of six analyses per sample. Yellow dent corn. Enogen Feed corn, Syngenta Seeds, LLC, Downers Grove, IL. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Table 2. Diet composition, Exp. 1 (as-fed basis) 1 2 Item Phase 1 Phase 2 Ingredient, % Corn 56.40 66.45 Soybean meal, 46.5% CP 24.70 29.75 Dried whey 10.00 --- Enzymatically treated soybean meal 5.00 --- Calcium carbonate 0.80 0.80 Monocalcium phosphate, 21% P 0.90 0.90 Sodium chloride 0.50 0.60 L-Lysine HCl 0.45 0.50 DL-Methionine 0.22 0.20 L-Threonine 0.18 0.23 L-Tryptophan 0.03 0.04 L-Valine 0.11 0.13 Trace mineral 0.15 0.15 Vitamin premix 0.25 0.25 Zinc oxide 0.25 --- Phytase 0.04 0.04 Total 100 100 Calculated analysis Standardized ileal digestible (SID) amino acids, % Lysine 1.35 1.30 Isoleucine:lysine 58 55 Leucine:lysine 116 115 Methionine:lysine 37 36 Methionine and cysteine:lysine 58 58 Threonine:lysine 63 64 Tryptophan:lysine 19.0 19.2 Valine:lysine 70 70 Histidine:lysine 36 37 Total lysine, % 1.49 1.44 NE, kcal/kg 2,445 2,560 Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 SID lysine:NE, g/Mcal 5.52 5.34 Crude protein, % 21.2 20.5 Ca, % 0.71 0.66 P, % 0.62 0.58 Analyzed Ca:analyzed P 1.14 1.13 STTD P, % 0.50 0.45 Phase 1 diets were fed from approximately 6.8 to 11.3 kg. Phase 2 diets were fed from approximately 11.3 to 24.9 kg. Enogen Feed corn replaced conventional yellow dent corn on an equal weight basis. HP300 (Hamlet Protein, Findlay, OH). Provided per kg of premix: 73g Zn from Zn sulfate; 73 g Fe from iron sulfate; 22 g Mn from manganese oxide; 11 g Cu from copper sulfate; 0.2 g I from calcium iodate; 0.2 g Se from sodium selenite. Provided per kg of premix: 3,527,399 IU vitamin A; 881,850 IU vitamin D; 17,637 IU vitamin E; 1,764 mg vitamin K; 15.4 mg vitamin B12; 33,069 mg niacin; 11,023 mb pantothenic acid; 3,307 mg riboflavin. HiPhos 2700 (DSM Nutritional Products, Parisppany, NJ) provided an estimated release of 0.10% STTD P. NE = net energy. STTD P = standardized total tract digestible phosphorus. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Table 3. Diet composition, Exp. 2 (as-fed basis) 1 2 3 Item Phase 1 Phase 2 Phase 3 Ingredient, % Corn 75.45 81.90 85.25 Soybean meal, 46.5 % CP 21.80 15.65 12.35 Calcium carbonate 0.93 0.85 0.85 Monocalcium phosphate, 21% P 0.55 0.40 0.35 Sodium chloride 0.50 0.50 0.50 L-Lysine HCl 0.30 0.30 0.30 DL-Methionine 0.07 0.03 0.02 L-Threonine 0.09 0.10 0.11 L-Tryptophan 0.01 0.02 0.02 Trace mineral 0.15 0.13 0.10 Vitamin premix 0.15 0.13 0.10 Phytase 0.02 0.02 0.02 Total 100 100 100 Calculated analysis Standardized ileal digestible (SID) amino acids, % Lysine 0.95 0.80 0.72 Isoleucine:lysine 62 61 60 Leucine:lysine 139 148 154 Methionine:lysine 32 31 30 Methionine and cysteine:lysine 58 58 58 Threonine:lysine 63 65 68 Tryptophan:lysine 18.6 18.5 18.7 Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Valine:lysine 69 70 70 Histidine:lysine 42 43 43 Total lysine, % 1.07 0.90 0.82 Net energy, kcal/kg 2,487 2,529 2,551 SID lysine:net energy, g/Mcal 3.83 3.16 2.82 Crude protein, % 18.4 14.6 13.3 Ca, % 0.60 0.51 0.48 P, % 0.47 0.41 0.38 Analyzed Ca:analyzed P 1.27 1.25 1.26 STTD P, % 0.33 0.28 0.26 Phase 1 diets were fed from d 0 to 14. Phase 2 diets were fed from d 14 to 42. Phase 3 diets were fed from d 42 to 83. Enogen Feed corn replaced conventional corn on an equal weight basis. HiPhos 2700 (DSM Nutritional Products, Parisppany, NJ) provided an estimated release of 0.10% STTD P. Standardized total tract digestible phosphorus. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 1,2 Table 4. Particle size analysis of ground corn, Exp. 1 Conventional yellow dent corn Enogen Feed corn Item With flow agent Without flow agent With flow agent Without flow agent Particle size phase 1, µm 300 345 407 345 403 600 576 675 554 564 900 954 1,058 883 972 Particle size phase 2, µm 300 376 454 328 417 600 606 725 525 646 900 1,069 1,169 926 1,460 Ground corn samples were collected the day of feed manufacturing, values represent the mean of 2 samples. Ground corn samples were split using a riffle splitter to produce 2, 100 g samples. A dispersing agent (0.5 g of powdered synthetic amorphous silicon dioxide, Gilson Company, Inc., Lewis Center, OH) was added to one of the samples. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 1,2 Table 5. Particle size analysis of ground corn, Exp. 2 Conventional yellow dent corn Enogen Feed corn Without flow Item With flow agent agent With flow agent Without flow agent Particle size phase 1, µm 300 343 423 287 419 600 510 673 414 577 900 911 975 785 931 Particle size phase 2, µm 300 338 434 309 417 600 561 704 567 646 900 932 1,096 983 1,123 Particle size phase 3, µm 300 374 469 350 476 600 602 743 618 750 900 974 1,167 975 1,202 Ground corn samples were collected the day of feed manufacturing, values represent the mean of Ground corn samples were split using a riffle splitter to produce 2, 100 g samples. A dispersing agent (0.5 g of powdered synthetic amorphous silicon dioxide) was added to one of the samples. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Table 6. Chemical analysis of diets, Exp. 1, (as-fed basis) Phase 1 Phase 2 3 4 Item, % Conventional Enogen Feed corn Conventional Enogen Feed corn Dry matter 90.32 90.78 89.41 89.42 Crude protein 20.95 20.55 20.35 19.85 Acid detergent fiber 3.10 3.00 3.10 3.30 Neutral detergent fiber 6.50 6.80 5.70 6.60 Ca 0.76 0.81 0.73 0.78 P 0.54 0.52 0.48 0.51 Feed samples were collected approximately 2 days after each feed delivery, pooled within corn source for each phase, and analyzed. (Ward Laboratories, Inc., Kearney, NE). The experimental diets were fed in 2 phases: d 0 to 14, and d 14 to 35. Yellow dent corn. Enogen Feed corn, Syngenta Seeds, LLC, Downers Grove, IL. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Table 7. Chemical analysis of diets, Exp. 2, (as-fed basis) Phase 1 Phase 2 Phase 3 Conventiona 3 4 Item, % Conventional Enogen Feed corn l Enogen Feed corn Conventional Enogen Feed corn Dry matter 89.15 88.62 88.22 88.53 88.04 88.69 Crude protein 17.25 16.3 14.35 13.65 12.3 12.6 Acid detergent fiber 2.55 2.75 2.65 2.45 2.15 2.35 Neutral detergent fiber 5.55 5.65 4.90 5.75 5.25 5.65 Ca 0.72 0.73 0.76 0.71 0.59 0.59 P 0.42 0.40 0.37 0.34 0.33 0.33 Feed samples were collected approximately 2 days after each fe ed delivery, pooled within corn source fo r each phase, and analyzed. (Ward Laboratories, Inc., Kearney, NE). The experimental diets were fed in 3 phases: d 0 to 14, d 14 to 42, and d 42 to 83. Yellow dent corn. Enogen Feed corn, Syngenta Seeds, LLC, Downers Grove, IL. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Table 8. Effects of corn source and particle size on growth performance of weanling pigs, Exp. 1 2 3 Conventional, µm Enogen Feed corn, µm Probability, P = Source × particle size, Source × particle size, Item 300 600 900 300 600 900 SEM linear quadratic BW , kg d 0 6.6 6.6 6.6 6.6 6.6 6.6 0.05 0.754 0.856 d 14 11.7 11.7 11.7 12.1 12.3 12.0 0.20 0.911 0.467 d 35 23.9 23.8 23.5 23.9 24.2 23.3 0.33 0.749 0.283 d 0 to 14 ADG , g 366 363 356 394 405 386 12.3 0.939 0.497 ADFI, g 448 440 440 459 483 482 14.4 0.237 0.472 G:F, g/kg 818 825 810 860 841 800 11.6 0.027 0.945 d 14 to 35 ADG, g 571 577 562 560 569 540 9.4 0.530 0.584 ADFI, g 907 898 900 882 939 902 19.3 0.408 0.071 G:F, g/kg 631 644 625 636 607 600 9.8 0.095 0.125 d 0 to 35 ADG, g 489 490 477 494 503 478 8.9 0.801 0.462 Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 ADFI , g 722 712 711 713 757 734 15.3 0.203 0.086 G:F , g/kg 677 689 672 693 666 652 8.0 0.027 0.127 A total of 360 pigs (Line 200 × 400, DNA, Columbus, NE initially 6.6 kg) with 12 pens per treatment and 5 pigs per pen. Yellow dent corn. Enogen Feed corn, Syngenta Seeds, LLC, Downers Grove, IL. BW = body weight. The experimental diets were fed in 2 phases: d 0 to 14, and d 14 to 35. ADG = average daily gain; ADFI = average daily feed intake; G:F = gain to feed. Conventional yellow dent corn when particle size of ground corn was reduced (linear, P = 0.640), Enogen Feed corn when particle size was reduced (quadratic, P = 0.030). Convetional yellow dent corn when particle size of ground corn was reduced (linear, P = 0.610), Enogen Feed corn when particle size was reduced (linear, P = <0.001). Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Table 9. Main effects of corn source and particle size on growth performance of weanling pigs, Exp. 1 Source Particle size, µm Probability, P = 2 3 Conventional Enogen Feed corn SEM Probability, P = 300 600 900 SEM Linear Quadratic Item BW , kg d 0 6.6 6.6 0.05 0.778 6.6 6.6 6.6 0.05 0.531 0.612 d 14 11.7 12.1 0.15 0.001 11.9 12.0 11.8 0.17 0.498 0.394 d 35 23.7 23.8 0.25 0.731 23.9 24.0 23.4 0.27 0.091 0.131 d 0 to 14 ADG , g 362 395 7.9 0.001 380 384 371 9.2 0.424 0.391 ADFI, g 442 475 10.0 0.003 453 461 461 11.3 0.563 0.712 G:F, g/kg 818 834 6.7 0.094 839 833 805 8.2 0.006 0.268 d 14 to 35 ADG, g 570 556 6.2 0.063 566 573 551 7.1 0.101 0.064 ADFI, g 902 908 11.4 0.641 895 919 901 15.3 0.708 0.160 G:F, g/kg 633 614 5.9 0.016 634 626 613 6.7 0.036 0.757 d 0 to 35 Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 ADG, g 485 492 6.3 0.309 491 497 478 7.1 0.090 0.074 ADFI, g 715 734 11.3 0.062 718 734 723 12.5 0.681 0.190 G:F, g/kg 679 671 4.6 0.143 685 678 662 5.7 0.005 0.573 A total of 360 pigs (Line 200 × 400, DNA, Columbus, NE, initially 6.6 kg) with 12 pens per treatment and 5 pigs per pen. Yellow dent corn. Enogen Feed corn, Syngenta Seeds, LLC, Downers Grove, IL. BW = body weight. The experimental diets were fed in 2 phases: d 0 to 14, and d 14 to 35. ADG = average daily gain; ADFI = average daily feed intake; G:F = gain to feed. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Table 10. Effects of corn source and particle size on growth performance and carcass characteristics of finishing pigs, Exp. 2 2 3 Conventional, µm Enogen Feed corn, µm Probability, P = Source × particle size, Source × particle size, Item 300 600 900 300 600 900 SEM linear quadratic BW , kg d 0 50.6 50.3 49.8 49.8 48.7 49.7 1.00 0.554 0.228 d 28 78.9 78.6 77.8 78.1 78.3 77.9 0.31 0.314 0.339 d 56 107.1 106.4 104.9 106.6 107.4 105.4 0.63 0.380 0.849 d 83 134.7 132.0 133.1 135.4 134.2 132.1 1.33 0.669 0.600 d 0 to 28 ADG , g 1,040 1,034 1,006 1,012 1,019 1,004 16.8 0.388 0.883 ADFI, g 2,217 2,250 2,294 2,199 2,323 2,288 41.4 0.731 0.484 G:F, g/kg 468 460 439 460 440 439 6.6 0.681 0.250 d 28 to 56 ADG, g 1,004 994 967 1,020 1,039 981 21.2 0.960 0.366 ADFI, g 2,886 2,950 2,948 2,946 2,984 3,025 44.7 0.837 0.622 G:F, g/kg 348 337 328 346 348 324 5.2 0.821 0.140 Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 d 56 to 83 ADG, g 969 939 945 983 973 941 22.9 0.645 0.397 ADFI, g 2,965 2,965 3,079 3,048 3,118 3,090 55.0 0.485 0.248 G:F, g/kg 327 317 308 322 312 305 5.3 0.913 0.915 d 0 to 83 ADG, g 1,006 990 974 1,006 1,011 976 11.7 0.922 0.327 ADFI, g 2,682 2,714 2,766 2,719 2,799 2,793 34.3 0.957 0.491 G:F, g/kg 375 365 352 370 361 350 3.4 0.895 0.801 Carcass characteristics HCW , kg 100.0 98.1 97.8 100.7 99.7 97.6 1.70 0.754 0.600 0.00 Carcass yield, % 74.1 74.2 73.6 74.3 74.1 73.8 2 0.960 0.259 0.49 Backfat depth, mm 16.3 16.3 16.4 16.2 16.4 16.5 8 0.871 0.930 0.93 Loin depth, mm 67.4 65.2 65.1 66.0 65.1 66.4 7 0.171 0.989 0.29 Lean, % 54.8 54.4 54.4 54.6 54.4 54.6 5 0.519 0.891 A total of 323 mixed gender pigs (Line 241 × 600, DNA, Columbus, NE, initially 50.0 ± 0.3 kg) were used with 9 pigs per pen and 6 pens per Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 treatment. Conventional yellow dent. Enogen Feed corn, Syngenta Seeds, LLC, Downers Grove, IL. BW = body weight. ADG = average daily gain; ADFI = average daily feed intake; G:F = gain to feed. HCW = hot carcass weight. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Table 11. Main effects of corn source and particle size on growth performance and carcass characteristics of finishing pigs, Exp. 2 Source Particle size, µm Probability, P = Enogen Probability, P Conventional SEM 300 600 900 SEM Linear Quadratic Item Feed corn = BW , kg d 0 50.2 49.4 0.90 0.091 50.2 49.5 49.8 0.93 0.457 0.324 d 28 78.9 77.7 1.04 0.064 78.9 78.1 77.9 1.09 0.164 0.702 d 56 106.6 106.1 1.01 0.494 107.2 106.6 105.1 1.08 0.031 0.564 d 83 133.6 133.5 1.17 0.933 135.4 132.8 132.6 1.32 0.057 0.328 d 0 to 28 ADG , g 1,028 1,010 10.6 0.185 1,027 1,025 1,005 12.7 0.187 0.497 ADFI, g 2,266 2,260 32.8 0.875 2,220 2,279 2,290 38.2 0.133 0.541 G:F, g/kg 454 448 5.5 0.231 462 451 439 6.2 0.002 0.929 d 28 to 56 ADG, g 988 1014 13.1 0.128 1,012 1,017 974 15.6 0.067 0.177 Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 ADFI, g 2,929 2,984 25.9 0.131 2,917 2,966 2,987 32.0 0.125 0.702 G:F, g/kg 338 340 3.0 0.615 347 343 326 3.7 0.001 0.166 d 56 to 83 ADG, g 949 967 13.4 0.349 975 957 943 16.7 0.187 0.932 ADFI, g 3,001 3,086 31.8 0.064 3,004 3,043 3,085 39.4 0.151 0.975 G:F, g/kg 317 313 3.1 0.512 324 315 306 3.9 0.003 0.979 d 0 to 83 ADG, g 990 998 6.7 0.398 1,005 1,001 975 8.3 0.014 0.289 ADFI, g 2,724 2,767 20.4 0.145 2,701 2,756 2,779 39.4 0.043 0.691 G:F, g/kg 364 360 3.1 0.368 372 363 350 3.9 0.001 0.419 Carcass characteristics HCW , kg 98.6 99.3 1.233 0.576 100.3 98.9 97.7 1.355 0.093 0.919 Carcass yield, % 74.0 74.1 0.001 0.437 74.2 74.1 73.7 0.002 0.023 0.253 Backfat depth, mm 16.4 16.3 0.304 0.965 16.5 16.4 16.2 0.357 0.645 0.949 Loin depth, mm 65.9 65.8 0.615 0.921 66.7 65.7 65.2 0.728 0.317 0.193 Lean, % 54.5 54.5 0.175 0.955 54.7 54.4 54.5 0.213 0.509 0.482 A total of 323 mixed gender pigs (Line 241 × 600, DNA, Columbus, NE, initially 50.0 ± 0.3 kg) with 9 pigs per pen and 6 pens per treatment. Yellow dent corn. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Enogen Feed corn, Syngenta Seeds, LLC, Downers Grove, IL. BW = body weight. ADG = average daily gain; ADFI = average daily gain; G:F = gain to feed. HCW = hot carcass weight. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Table 12. Effects of particle size on stomach ulceration and keratinization for Exp. 2 2 3 Conventional Enogen Feed corn Probability Source × particle Item 300 600 900 300 600 900 size Source Particle size Ulcer score 1.42 1.42 1.67 1.92 1.50 1.42 0.178 0.438 0.840 Keratinization score 2.92 2.00 1.58 1.42 1.92 1.17 0.055 0.002 0.015 On d 79, 2 pigs per pen, 1 barrow and 1 gilt of equal weight, were selected and transported to Natural Food Holdings, Sioux Center, Iowa to collect stomachs. The stomachs were taken to the Kansas State University diagnostic lab where a scoring system was used to determine the severity of ulceration and keratinization of the esophageal opening of the stomach. Yellow dent corn. Enogen Feed corn, Syngenta Seeds, LLC, Downers Grove, IL. Stomachs were scored on a scale of 1 to 4 with 1 = no ulceration, 2 = <25% ulceration, 3 = 25-75% ulceration and 4 > 75%. This scoring criteria used was the same for keratinization. Accepted Manuscript http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Translational Animal Science Oxford University Press

The influence of particle size of Enogen Feed corn and conventional yellow dent corn on nursery and finishing pig performance, carcass characteristics and stomach morphology

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Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 The influence of particle size of Enogen Feed corn and conventional yellow dent corn on nursery and finishing pig performance, carcass characteristics and stomach morphology Hadley R. Williams *, Mike D. Tokach*, Jason C. Woodworth*, Joel M. DeRouchey*, Robert D. Goodband*, Jordan T. Gebhardt†, and Chad B. Paulk‡ *Department of Animal Sciences and Industry, College of Agriculture, Kansas State University, Manhattan, KS 66506 USA †Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506 USA ‡Department of Grain Science and Industry, College of Agriculture, Kansas State University, Manhattan, KS 66506 USA 1 Corresponding author: hadley1@ksu.edu © The Author(s) 2021. Published by Oxford University Press on behalf of the American Society of Animal Science. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 ABSTRACT: Enogen Feed corn is a variety developed by Syngenta Seeds (Downers Grove, IL) that has been genetically modified to contain an α-amylase enzyme trait (SYT-EFC). Originally, Enogen feed corn was developed for the ethanol industry due to its reduction in viscosity of the corn mash, thus eliminating the need to add a liquid form of the α-amylase enzyme. However, there is potential application for Enogen Feed corn to be used in livestock diets due to the increase in α-amylase enzyme potential to increase starch digestibility. A more common method of increasing starch digestibility in corn is to finely grind it to reduce particle size. This increases the surface area and allows for greater interaction with digestive enzymes. We hypothesized that pigs fed Enogen feed corn potentially could achieve similar gain:feed ratio (G:F) at larger particle sizes than conventional corn because of the differences in starch digestibility. In Exp. 1, a total of 360 pigs (DNA 200 × 400, Columbus, NE; initially 6.6 ± 0.1 kg BW) were used with 5 pigs per pen and 12 pens per treatment. Treatments were arranged in a 2 × 3 factorial with main effects of corn source (Enogen Feed corn or conventional yellow dent corn) and ground corn particle size (300, 600, or 900 µm). Overall, there was a corn source × particle size interaction (linear, P = 0.027) for G:F. There was no effect due to particle size when pigs were fed conventional yellow dent corn, but in pigs fed Enogen Feed corn, G:F increased with decreasing particle size. Neither corn source nor particle size affected (P > 0.05) overall average daily gain (ADG) or average daily feed intake (ADFI). In Exp. 2, a total of 323 pigs (241  600; DNA, Columbus, NE; initially 50.0 ± 1.3 kg) were used with 9 pigs per pen and 6 pens per treatment. Treatments were identical as Exp. 1. Overall, corn source had no effect on finishing pig ADG, ADFI or G:F. For corn particle size, ADG and G:F increased (linear, P < 0.014) and ADFI decreased (P = 0.043) as particle size decreased. For stomach morphology, there was a tendency for a corn source × particle size interaction (P = 0.055) for Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 keratinization score with keratinization increasing linearly (P = 0.001) as particle size of the corn decreased for yellow dent corn with no change in keratinization score as particle size decreased for Enogen Feed corn. In summary, reducing corn particle size improved G:F with no major differences observed between corn sources for overall pig performance. Key words: α-amylase, Enogen Feed corn, finishing pigs, growth performance, nursery pigs, particle size Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 INTRODUCTION Cereal grains such as corn, grain sorghum, wheat, and barley typically provide most of the dietary energy in swine diets. Due to the elevated energy density and availability when compared to other cereal grains, corn is the most commonly used cereal grain among pork production systems in the United States. Corn typically contains 65% starch (NRC, 2012) with apparent total tract digestibility around 90 to 96% (Rojas and Stein, 2015). Genetic modification of corn varieties has enhanced nutrient profiles. Genetically modified corn varieties such as NutriDense and low-phytate corn, have been previously evaluated in growing-finishing pigs (Spencer et al., 2000, Hastad et al., 2005). Enogen Feed corn, (Syngenta Seeds, Downers Grove, IL), has been genetically enhanced to contain a thermotolerant α-amylase enzyme trait. While Enogen Feed corn was originally intended to be used by the ethanol industry, the increase in α-amylase may have application in livestock feeding. Recent trial conducted by Ochonski et al. (2019) determined that utilizing Enogen Feed corn in finishing pig diets tended to increase average daily gain compared to pigs that were fed diets with conventional corn, but there was no influence on feed efficiency. Although corn varieties can influence growth performance, reducing its particle size can influence performance as well. Grinding corn to reduce particle size increases the surface area, allowing more enzymatic activity to occur and increase the digestibility of both energy and other nutrients (Kim et al., 2002). Research in finishing pigs has shown that reducing corn particle size from 1,000 to 400 µm improves feed efficiency (Wondra et al., 1995). However, decreasing particle size below 700 µm is not always beneficial. Wondra et al. (1995) suggested reducing particle size causes more lesions and greater keratinization of the esophageal region due to the lack of protective mucus in this region of the stomach. We Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 hypothesized that feeding coarse Enogen Feed corn will have the same response as feeding fine particle size conventional corn due to the higher availability of starch. Therefore, the objective of this study was to determine the effects of feeding Enogen Feed corn compared to conventional yellow dent corn at different particle sizes on nursery and finishing pig growth performance, carcass characteristics and stomach morphology. MATERIALS AND METHODS The Kansas State University and Institutional Animal Care and Use Committee approved the protocols used in these experiments. Ingredients and chemical analysis All diets for experiment 1 and 2 were manufactured at the Kansas State University O.H. Kruse Feed Technology Innovation Center (Manhattan, KS). All corn in this study was source from a single source for each corn type. Conventional U.S. No. 2 dent corn and Enogen Feed corn were ground to approximately 300, 600 or 900 µm using a roller mill (Model 924, RMS Roller Grinder, Harrisburg, SD) equipped with 3 roll pairs. The top and middle roll pairs had a speed differential of 1.5 with the front rolls operating at 1,285 rpm and the back rolls at 850 rpm. The bottom roll pair differential was 0.68 with the front roll operating at 1,020 rpm and the back roll at 1,500 rpm. The top, middle, and bottom roll pairs had 2.36 and 2.36 corrugations/cm, 4.72 and 5.51 corrugations/cm, and 6.30 and 7.09 corrugations/cm, respectively. Adjustments were made to the roll gap setting to achieve the desired particle sizes. Samples of ground corn were obtained during each feed manufacturing event for each experiment and analyzed (Table 1; Ward Laboratories, Inc., Kearney, NE) for dry matter (method 935.29; AOAC Inc., Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 2019), starch (Application #322. 2000), crude protein (method 990.03; AOAC Inc., 2019), ether extract (ANKOM Technology, 2004), acid detergent fiber and neutral detergent fiber (ANKOM Technology, 2005), calcium (method 6.3; Kovar, 2003) and phosphorus (method 6.3; Kovar, 2003). Particle size analysis was conducted on ground corn samples (100 g) in duplicate according to the ANSI/ASAE S319.2 (1995) standard method. Samples were analyzed both with (5 g) and without dispersing agent (Gilson Company, Inc., Lewis Center, OH) using 2 separate stainless-steel sieve stacks (13 sieves each) to prevent residual dispersing agent from affecting results. Both sieve stacks contained agitators and were placed in the Ro-Tap (Model RX-29, W. S. Tyler Industrial Group, Mentor, OH) machine for 15 min, using the same sieve and agitator configurations as Gebhardt et al. (2018). Representative diet samples from each manufacturing event were obtained from each treatment within experiment and stored at -20C until analysis. Diet samples were analyzed (Ward Laboratories, Inc., Kearney, NE) for dry matter, crude protein, acid detergent fiber, neutral detergent fiber, Ca, and P using the same procedures as used for ground grain samples. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Animals and diets In both experiments, treatments were arranged in a 2  3 factorial with main effects of corn source (conventional yellow dent corn or Enogen Feed corn) and ground corn particle size (300, 600 or 900 µm). Diets were similar between all treatments, and Enogen Feed corn replaced conventional yellow dent corn on an equal weight basis (Tables 2 and 3). Experiment 1 was conducted at the Kansas State University Segregated Early Weaning facility (Manhattan, KS). A total of 360 barrows (DNA 200 × 400, Columbus, NE; initially 6.6 ± 0.01 kg) were placed in pens with 5 pigs per pen. Pens (1.22  1.22 m) had metal slatted floors and were equipped with a four-hole stainless steel dry feeder and a water cup. Pigs were weaned at approximately 21 d of age and placed in pens based on initial body weight (BW), and fed a common pelleted started diet for approximately 7-d. On d 7, which was considered d 0 of the study, pens of pigs were allotted to 1 of 6 dietary treatments in a randomized complete block design with BW as the blocking factor. There were 12 replications (pens) per treatment. Diets were provided ad libitum in mash form and split into 2 separate phases. Phase 1 was fed from d 0 to 14 and phase 2 was fed from d 14 to 35. Pens of pigs were weighed, and feed disappearance was measured weekly to calculate average daily gain (ADG), average daily feed intake (ADFI), and gain to feed ratio (G:F). Experiment 2 was conducted at the Kansas State University Swine Teaching and Research Center (Manhattan, KS). A total of 323 pigs (241  600; DNA, Columbus, NE; initially 50.0 ± 1.3 kg) were used in an 83-d trial. Pens of pigs were allotted to 1 of 6 dietary treatments in a randomized complete block design with BW as the blocking factor with 9 pigs per pen and 6 pens per treatment. Diets were fed in mash form in 3 separate phases. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 The facility was totally enclosed and environmentally regulated, containing 36 pens. Each pen (3.00 × 2.44 m) was equipped with a 1-cup waterer and a dry, single-sided feeder (Farmweld, Teutopolis, IL) with two feeder spaces. Pens were located over a completely slatted concrete floor with a 1.22-m deep pit underneath for manure storage. A robotic feeding system (FeedPro; Feedlogic Corp., Wilmar, MN) was used to deliver and record daily feed additions to each pen. Pens were equipped with adjustable gates to allow space allowance per pig to be maintained if a pig died or was removed during the experiment. Growth performance was assessed by recording BW and feed disappearance every 2 weeks and at the conclusion of the study (d 83). On d-79, 2 pigs per pen, 1 barrow and 1 gilt of equal weight based on average of the pen weight, were selected and transported to a USDA inspected packing facility (Natural Food Holdings, Sioux Center, IA) for slaughter and to collect stomachs. The stomachs were taken to the Kansas State University Veterinary Diagnostic Laboratory where a scoring system was used to determine the severity of ulceration and keratinization of the esophageal opening of the stomach (DeJong, 2016). Stomach ulceration score was based on a scale of 1 to 4, with 1 = no ulceration, 2 = < 25% ulceration, 3 = 25-75% ulceration and 4 = > 75% ulceration. This scoring criteria was the same for keratinization. On d 83, the remaining pigs were individually tattooed with a unique ID number, and a radio frequency identification (RFID) transponder was inserted into the right ear to allow carcass measurements to be recorded on an individual pig basis. Final individual pig weights were taken, and pigs were transported approximately 2.5 h to a commercial packing plant (Triumph Foods, St. Joseph, MO) and held in lairage for approximately 7 h before slaughter. At the plant, hot carcass weight (HCW) was determined immediately after evisceration. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Backfat and loin depth were measured with an optical probe (Fat-O-Meter, SFK, Herlev, Denamrk) inserted between the third and fourth rib (counting from the ham end of the carcass) at a distance approximately 7 cm from the dorsal midline. Percentage lean was calculated using proprietary equations from the packing plant. Carcass yield was calculated by dividing the individual HCW obtained from the packing plant by the individual final live weight obtained at the farm. Statistical Analysis Treatments were analyzed as a randomized complete block design for two-way ANOVA using the lmer function from the lme4 package in R (version 3.5.1 (2018-07-.2)) with pen considered the experimental unit, average pen body weight at allotment to treatment diets as blocking factor included in the model as a random effect, and treatment as fixed effect. The main effects of corn source and particle size, as well as their interactions, were tested. Differences between treatments were considered significant at P ≤ 0.05, and a tendency for significance at 0.05 < P ≤ 0.10. RESULTS Chemical and particle size analysis The chemical analysis for ground corn was similar across sources for both Exp. 1 and 2 (Table 1). Samples analyzed with dispersing agent resulted in lower geometric mean diameter (dgw) and greater geometric standard deviation (sgw) than those analyzed without agent as expected (Table 4 and 5). Kalivoda et al. (2017) observed similar findings when comparing methods and suggested that the inclusion of a dispersing agent best facilitated the movement of fine material through sieves and reduced possible Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 agglomerations due to forces such as static charge. Thus, particle size targets and roller mill settings for both trials were based on the analysis with the inclusion of dispersing agent. For Exp. 1, the target particle sizes were achieved and remained similar when comparing conventional to Enogen feed corn (Table 4). For Exp. 2, the particle size targets were again achieved and remained similar across corn source with the exception of a lower than expected dgw in phase 1 for the highest particle size for Enogen feed corn (785 µm) when compared to the closer to target conventional corn (911 µm; Table 5). Complete diet nutrient analysis matched expected values for Exp. 1 and 2 (Table 6 and 7). Experiment 1 Interactive and main effects are reported in Tables 8 and 9. During phase 1 (d 0 to 14), there was no evidence for interaction or main effect differences for ADG and ADFI for ground corn particle size. However, there was a corn source × particle size interaction (linear, P = 0.027) for G:F. There were no changes in G:F for pigs fed decreasing particle size of conventional yellow dent corn, but a linear improvement in G:F was observed as particle size decreased for pigs fed Enogen Feed corn (P < 0.001). During phase 2 (d 14 to 35), there was no evidence for differences in ADG among pigs fed either corn source or different particle sizes. There was a tendency for a corn source × particle size interaction (quadratic, P = 0.071) for ADFI, with no differences in ADFI for pigs fed conventional yellow dent corn as particle size changed (P = 0.540), but an increase then decrease in ADFI as particle size increased in pigs fed Enogen Feed corn. There was also a tendency for a corn source × particle size interaction (linear, P = 0.095) for G:F. As particle size of conventional yellow dent corn was reduced from 900 to 600 micron we observed an improvement in G:F and Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 then decreased as the particle size was reduced from 600 to 300 micron. For pigs fed Enogen Feed corn, as particle size was reduced from 900 to 300 micron G:F was improved. There was no difference among pigs fed conventional yellow dent corn, but G:F improved as particle size decreased in pigs fed Enogen Feed corn. For overall performance (d 0 to 35), there was no evidence for differences in ADG due to corn source or particle size. There was a tendency for a corn source × particle size interaction (quadratic, P = 0.086) for ADFI. There was no change in ADFI in pigs fed conventional yellow dent corn regardless of corn particle size, whereas pigs fed Enogen Feed corn had an increase then decrease in ADFI as particle size increased. For overall G:F, there was also a corn source × particle size interaction (linear, P = 0.027). There was no difference in G:F when pigs were fed conventional yellow dent corn regardless of corn particle size, but for pigs fed Enogen Feed corn, G:F improved with decreasing particle size. Experiment 2 There was no evidence for a corn source × particle size interaction for any growth response criteria (Table 10). There was no evidence for difference in growth performance or carcass characteristics between corn sources, except for a tendency for greater (P = 0.064) ADFI from d 56 to 83 of the experiment for pigs fed Enogen Feed corn compared with pigs fed conventional yellow dent corn. For BW, there was a tendency for greater (P < 0.10) BW on d 0 and 28 for pigs fed conventional yellow dent corn compared to pigs fed Enogen Feed corn. This small initial difference disappeared by d 56 with no differences in BW between pigs fed either corn source for the remainder of the experiment. As expected, G:F improved (linear, P < 0.05) during each phase and overall as corn particle size decreased. Overall, ADFI decreased (linear, P = 0.043) as corn particle size decreased (Table 11) while ADG increased Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 (linear, P = 0.014) as particle size decreased. The improvement in ADG led to a tendency for increased (linear, P = 0.056) BW on d 83 as corn particle decreased. For carcass characteristics, there was a tendency for an increase (linear, P = 0.093) in HCW as corn particle size decreased in the diet. Carcass yield also increased (linear, P = 0.023) as corn particle size decreased. For stomach morphology (Table 12), there was a tendency for a corn source × particle size interaction (P = 0.055) for keratinization score. This was caused by a lower incidence of keratinization in stomachs of pigs fed Enogen Feed corn when ground to 300 or 900 µm than pigs fed conventional yellow dent corn at these particle sizes with similar keratinization score when both corn sources were ground to 600 µm. Neither corn source nor particle size affected stomach ulceration. DISCUSSION The United States is the world’s largest producer of corn, with an annual production of approximately 13 billion bushels in 2019 (NCGA, 2020). Corn in the United States is generally grown for 3 major uses: food production, livestock use, and the ethanol industry. A new corn hybrid, developed by Syngenta Seeds, Enogen Feed corn, was originally developed with the intent to be used in the ethanol industry. Enogen Feed corn has the ability to reduce the viscosity of its corn mash, thus eliminating the need to add a liquid form of the α-amylase enzyme. The chemical analysis of ground corn and complete experimental diets found an increase in starch concentration for Exp.1 for ground Enogen Feed corn and an increase in neutral detergent fiber (NDF) in both the Enogen Feed corn ground corn sample and complete experimental diets. Neutral detergent fiber is the general class of polysaccharides Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 that includes most pentosans but do not include certain soluble fibers such as arabino-xylan. Neutral detergent fibers are resistant to digestion by endogenous enzymes and are slowly but incompletely fermented to volatile fatty acids in the large intestine. An increase in dietary NDF decreases energy availability, causing increased feed intake to meet the nutrient requirements for the pig (Sauber and Owens, 2000). Starch is the major storage carbohydrate of cereal grains. Corn being the most prevalent of cereal grains has a starch content of 65% (NRC, 2012). The digestion of starch is first initiated when feed interacts with salivary amylase (Englyst and Hudson, 2000). Digestion by salivary amylase is short, due to the decrease of pH in the stomach as feed is ingested. Most of the starch digestion takes place in the small intestine, where the starch molecule is hydrolyzed into maltose, maltotriose, and isomaltose subunits by pancreatic α- amylase and isomaltase enzymes (Groff and Gropper, 2000). The digestion of starch is an efficient process, with approximately 95% of starch digestion occurring in the small intestine (Rojas and Stein, 2016). Enogen Feed corn contains an α-amylase enzyme trait (SYT-EFC). The SYT-EFC α-amylase enzyme is thought to help with the starch degradation and improve the digestibility of corn by converting starch into fermentable sugars. Although there is limited research on starch digestion of Enogen Feed corn in monogastrics, a study by Jolly- Breithaupt et al. (2016) observed when finishing cattle were feed corn with the SYT-EFC α- amylase enzyme, there was a numerical increase in post-ruminal starch digestibility compared to cattle fed conventional yellow dent corn. One of the main drivers of improved feed efficiency is an increase in the ability to utilize energy in the diet (Patience et al., 2016). With the increase of α-amylase in Enogen Feed corn, starch can potentially be converted to sugar more readily, allowing more energy Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 for the animal which in turn should improve feed efficiency. Ochonski et al. (2019) evaluated the use of Enogen Feed corn fed to finishing pigs. While they did not observe any difference in G:F, they observed a numerical increase in ADG when pigs were fed Enogen Feed corn compared to conventional yellow dent corn. In our study, we did not observe any significant differences for ADG, ADFI or G:F when comparing conventional yellow dent corn and Enogen Feed corn on overall finishing pig performance. Feed processing of cereal grains also can influence feed efficiency. The main reason cereal grains, like corn, are ground to fine particle sizes is to increase particle surface area and energy utilization (Wondra et al., 1995). A study conducted by Rojas and Stein (2015) evaluated the apparent ileal digestibility (AID) of starch and confirmed that decreased corn particle size from 865 to 339 µm linearly increased AID of starch from 89.0% to 96.6%. This is likely due to increased access to the starch granules for α-amylase, which increases starch digestibility in the small intestine. As digestibility increases, feed intake may also decrease because less feed is needed to meet the animal’s nutrient requirements. Although not statistically significant, in both experiments, we observed a numerical decrease in feed intake as corn particle size decreased. For nursery pigs in Exp. 1, as corn particle size was reduced from 900 to 300 µm for Enogen Feed corn, G:F improved linearly. Similarly, Bokelman et al. (2014) also observed improved G:F as diet particle size was reduced. However, a study conducted by DeJong et al. (2014) observed no improvement in G:F when the particle size of corn was reduced from approximately 900 µm to approximately 325 µm for nursery pigs. The reasons DeJong et al. (2014) observed no improvement in G:F was due to decreased ADG and ADFI when the corn was ground to very fine particle sizes. In our study we observed a tendency for a quadratic Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 effect in ADG, with pigs fed 300 and 600 µm corn having increased gain compared to the 900 µm, resulting in a linear improvement in G:F as corn particle size was decreased. When a pig is weaned, their enzyme activity is relatively low for enzymes like amylase and protease. Amylase is an endogenous enzyme that originates from saliva and brush border enzymes and is used to break down starch in the small intestine. In our study, we observed an improvement in ADG and G:F when pigs were fed Enogen Feed corn compared with pigs fed conventional yellow dent corn the 14 days post weaning. The increase in gain with pigs fed Enogen Feed corn could have been related to the increase in α-amylase in the corn. A study conducted by Yi et al., (2013) found an increase in weight gain when supplementing an enzyme complex of amylase, protease and xylanase to wean pigs. In our study, the improvement in gain, however, was not maintained during the subsequent period from d 14 to35 after weaning. Due to a large volume of feed consumed by finishing pigs over their life span, a small improvement in feed efficiency can result in large economic benefits. A study conducted by DeJong et al. (2013) with finishing pigs observed an improvement in G:F when reducing corn particle size from 596 to 320 µm. Other studies have shown that reducing the particle size of corn in the diet can decrease ADFI when fed to finishing pigs (DeJong et al., 2013, Nemechek et al., 2016). In Exp. 2, reducing corn particle size from 900 to 300 µm resulted in an increase in ADG, decrease in ADFI, and improvement in G:F. In the current study, a 1% improvement of G:F for every 100 µm decrease of ground corn was observed, which is consistent to the findings by Wondra et al. (1995) who saw an 8% improvement as corn particle size was reduced from 1,000 to 400 µm. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 While reducing corn particle size for finishing pigs has been shown to improve G:F, grinding corn to a fine particle has been shown to cause gastric lesions (Maxwell et al., 1970). In Exp. 2, there was no evidence for difference in corn source or particle size on ulcer score. However, pigs fed Enogen Feed corn had decreased keratinization score compared to pigs fed conventional yellow dent corn. As corn particle size was decreased from 900 to 300 µm, keratinization score increased for pigs fed conventional yellow dent corn. Keratinization, the process of keratin forming around the esophageal opening of the stomach, occurs prior to ulceration. Once the keratinized tissue is sloughed off, ulcerations are visible. Ulceration occurs with lower particle size due to reduced viscosity of the feed form in the stomach lowering the pH in the stomach. This difference in keratinization scores between corn sources warrants further investigation. In summary, Enogen Feed corn can be effectively used in nursery or finishing pig diets and result in less keratinization on stomach tissue than pigs fed conventional yellow dent corn. This data also confirms previous research that observed reducing particle size of corn improves G:F in both nursery and finishing pig diets. However, feeding Enogen Feed corn ground to coarser particle size did not yield the same response for G:F as conventional yellow dent corn at finer diet particle sizes as had been hypothesized. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 LITERATURE CITED ANKOM Technology. 2004. Rapid Determination of Oil/Fat Utilizing High Temperature Solvent Extraction. ANKOM Technology, Fairport, NY. ANKOM Technology. 2005. Method for Determining Crude Fiber. ANKOM Technology, Fairport, NY. st AOAC Int. 2019. Official methods of analysis AOAC international. 21 ed. Association of Official Analytical Chemists, Arlington, VA. ASADE Standards. 1995. S319.2: Method of determining and expressing fineness of feed materials by sieving. St. Joseph, MI: ASABE. 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W. Hong, M. R. Cabrera, R. H. Hines, and K. C. Behnke. 2002. Corn particle size affects nutritional value of simple and complex diets for nursery pigs and broiler chicks. Asian-Australs. J. Anim. Sci. 15, 872-877. doi:10.5713/ajas.2002.872. Kovar, J. L. 2003. Method 6.3 Inductively coupled plasma spectroscopy. University of Wisconsin-Extension, Madison, WI. Maxwell, C. V., E. M. Reimann, W.G. Hoekstra, T. Kowalcyzyk, N. J. Benevenga, and R. H. Grummer. 1970. Effect of dietary particle size on lesion development and on the contents of various regions of the swine stomach. J. Anim. Sci. 30-911-922. doi:10.2527/jas1970.306911x. National Corn Growers Association. 2019. Animal Ag facts. Date accessed: Aug. 17, 2020. Retrieved from: https://dt176nijwh14e.cloudfront.net/file/164/DYK-Animal%20Ag- 1.pdf. Nemechek, J. E., M. D. Tokach, S. S. Dritz, R. D. Goodband, J. M. DeRouchey, and J. C. Woodworth. 2016. Effects of diet form and corn particle size on growth performance and carcass characteristics of finishing pigs. Anim. Feed Sci. Technol. 214:136-141. doi:10.1016/j.anifeedsci.2016.02.002. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 th NRC. 2012. Nutrient Requirements of swine. 11 ed. Natl. Acad. Press, Washington, DC. doi:10.17226/13298. Ochonski, P., F. Wu, E. Arkfeld., J. M. Lattimer, J. M. DeRouchey, S. S. Dritz, R. D. Goodband, J. C. Woodworth, and M. D. Tokach. 2019. Evaluation of high amylase corn on growth performance and carcass characteristics of finishing pigs. Kansas Agricultural Experiment Station Research Reports: Vol. 5: Iss. 8. doi:10.4148/2378-5977.7850. Patience, J. F., M. C. Rossoni-Serao, and N. A. Gutierrez. 2016. A review of feed efficiency in swine: biology and application. J. Anim. Sci. Biotechn. 6:33. doi:10.1186/s40104-015- 0031-2. Rojas, O. J. and H. H. Stein. 2015. Effects of reducing the particle size of corn grain on the concentration of digestible and metabolizable energy and on the digestibility of energy and nutrients in corn grain fed to growing pigs. Livest Sci. 181:187-193. doi:10.1016/j.livsci.2015.09.013. Sauber, T. E. and F. N. Owens. 2000. Cereal Grains and By-Products for Swine. In: A. J. Lewis nd and L. L. Southern, editors, Swine Nutrition. 2 ed. CRC Press, Boca Raton, FL. p. 799- Spencer, J. D., G. L. Allee, and T. E. Sauber. 2000 Growing-finishing performance and carcass characteristics of pigs fed normal and genetically modified low-phytate corn. J. Anim. Sci. 78:1529-1536. doi:10.2527/2000.7861529x. Wondra, K. J., J. D. Hancock, K. C. Behnke, R. H. Hines, and C. R. Stark. 1995. Effects of particle size and pelleting on growth performance, nutrient digestibility, and Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 stomach morphology in finishing pigs. J. Anim. Sci. 73, 757-763. doi:10.2527/1995.733757x. Yi, J. Q., X. S. Piao, Z. C. Li, H. Y. Zhang, Y. Chen, Q. Y. Li, J. D. Liu, Q. Zhang, Y. J. Run, and B. Dong. 2013. The effects of enzyme complex on performance, intestinal health and nutrient digestibility of weaned pigs. Asian-Australas. J. Anim. Sci., 26, 1181-1188. doi:10.5713/ajas.2013.13129. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Table 1. Chemical analysis of corn varieties of Exp. 1 and 2 (as-fed basis) Experiment 1 Experiment 2 Conventional Enogen Feed Enogen Feed 2 3 Item, % corn Conventional corn Dry matter 87.38 87.73 87.72 87.80 Starch 59.68 64.15 60.60 59.76 Crude protein 7.74 7.45 7.68 7.40 Ether extract 4.10 4.10 3.88 4.16 Acid detergent fiber 1.70 1.91 1.55 1.73 Neutral detergent fiber 6.32 7.31 6.20 7.22 Ca 0.10 0.13 0.10 0.11 P 0.23 0.21 0.22 0.21 Corn samples were collected at time of feed manufacturing and pooled for analysis (Ward Laboratories, Inc., Kearney, NE). Each value represents the mean of six analyses per sample. Yellow dent corn. Enogen Feed corn, Syngenta Seeds, LLC, Downers Grove, IL. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Table 2. Diet composition, Exp. 1 (as-fed basis) 1 2 Item Phase 1 Phase 2 Ingredient, % Corn 56.40 66.45 Soybean meal, 46.5% CP 24.70 29.75 Dried whey 10.00 --- Enzymatically treated soybean meal 5.00 --- Calcium carbonate 0.80 0.80 Monocalcium phosphate, 21% P 0.90 0.90 Sodium chloride 0.50 0.60 L-Lysine HCl 0.45 0.50 DL-Methionine 0.22 0.20 L-Threonine 0.18 0.23 L-Tryptophan 0.03 0.04 L-Valine 0.11 0.13 Trace mineral 0.15 0.15 Vitamin premix 0.25 0.25 Zinc oxide 0.25 --- Phytase 0.04 0.04 Total 100 100 Calculated analysis Standardized ileal digestible (SID) amino acids, % Lysine 1.35 1.30 Isoleucine:lysine 58 55 Leucine:lysine 116 115 Methionine:lysine 37 36 Methionine and cysteine:lysine 58 58 Threonine:lysine 63 64 Tryptophan:lysine 19.0 19.2 Valine:lysine 70 70 Histidine:lysine 36 37 Total lysine, % 1.49 1.44 NE, kcal/kg 2,445 2,560 Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 SID lysine:NE, g/Mcal 5.52 5.34 Crude protein, % 21.2 20.5 Ca, % 0.71 0.66 P, % 0.62 0.58 Analyzed Ca:analyzed P 1.14 1.13 STTD P, % 0.50 0.45 Phase 1 diets were fed from approximately 6.8 to 11.3 kg. Phase 2 diets were fed from approximately 11.3 to 24.9 kg. Enogen Feed corn replaced conventional yellow dent corn on an equal weight basis. HP300 (Hamlet Protein, Findlay, OH). Provided per kg of premix: 73g Zn from Zn sulfate; 73 g Fe from iron sulfate; 22 g Mn from manganese oxide; 11 g Cu from copper sulfate; 0.2 g I from calcium iodate; 0.2 g Se from sodium selenite. Provided per kg of premix: 3,527,399 IU vitamin A; 881,850 IU vitamin D; 17,637 IU vitamin E; 1,764 mg vitamin K; 15.4 mg vitamin B12; 33,069 mg niacin; 11,023 mb pantothenic acid; 3,307 mg riboflavin. HiPhos 2700 (DSM Nutritional Products, Parisppany, NJ) provided an estimated release of 0.10% STTD P. NE = net energy. STTD P = standardized total tract digestible phosphorus. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Table 3. Diet composition, Exp. 2 (as-fed basis) 1 2 3 Item Phase 1 Phase 2 Phase 3 Ingredient, % Corn 75.45 81.90 85.25 Soybean meal, 46.5 % CP 21.80 15.65 12.35 Calcium carbonate 0.93 0.85 0.85 Monocalcium phosphate, 21% P 0.55 0.40 0.35 Sodium chloride 0.50 0.50 0.50 L-Lysine HCl 0.30 0.30 0.30 DL-Methionine 0.07 0.03 0.02 L-Threonine 0.09 0.10 0.11 L-Tryptophan 0.01 0.02 0.02 Trace mineral 0.15 0.13 0.10 Vitamin premix 0.15 0.13 0.10 Phytase 0.02 0.02 0.02 Total 100 100 100 Calculated analysis Standardized ileal digestible (SID) amino acids, % Lysine 0.95 0.80 0.72 Isoleucine:lysine 62 61 60 Leucine:lysine 139 148 154 Methionine:lysine 32 31 30 Methionine and cysteine:lysine 58 58 58 Threonine:lysine 63 65 68 Tryptophan:lysine 18.6 18.5 18.7 Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Valine:lysine 69 70 70 Histidine:lysine 42 43 43 Total lysine, % 1.07 0.90 0.82 Net energy, kcal/kg 2,487 2,529 2,551 SID lysine:net energy, g/Mcal 3.83 3.16 2.82 Crude protein, % 18.4 14.6 13.3 Ca, % 0.60 0.51 0.48 P, % 0.47 0.41 0.38 Analyzed Ca:analyzed P 1.27 1.25 1.26 STTD P, % 0.33 0.28 0.26 Phase 1 diets were fed from d 0 to 14. Phase 2 diets were fed from d 14 to 42. Phase 3 diets were fed from d 42 to 83. Enogen Feed corn replaced conventional corn on an equal weight basis. HiPhos 2700 (DSM Nutritional Products, Parisppany, NJ) provided an estimated release of 0.10% STTD P. Standardized total tract digestible phosphorus. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 1,2 Table 4. Particle size analysis of ground corn, Exp. 1 Conventional yellow dent corn Enogen Feed corn Item With flow agent Without flow agent With flow agent Without flow agent Particle size phase 1, µm 300 345 407 345 403 600 576 675 554 564 900 954 1,058 883 972 Particle size phase 2, µm 300 376 454 328 417 600 606 725 525 646 900 1,069 1,169 926 1,460 Ground corn samples were collected the day of feed manufacturing, values represent the mean of 2 samples. Ground corn samples were split using a riffle splitter to produce 2, 100 g samples. A dispersing agent (0.5 g of powdered synthetic amorphous silicon dioxide, Gilson Company, Inc., Lewis Center, OH) was added to one of the samples. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 1,2 Table 5. Particle size analysis of ground corn, Exp. 2 Conventional yellow dent corn Enogen Feed corn Without flow Item With flow agent agent With flow agent Without flow agent Particle size phase 1, µm 300 343 423 287 419 600 510 673 414 577 900 911 975 785 931 Particle size phase 2, µm 300 338 434 309 417 600 561 704 567 646 900 932 1,096 983 1,123 Particle size phase 3, µm 300 374 469 350 476 600 602 743 618 750 900 974 1,167 975 1,202 Ground corn samples were collected the day of feed manufacturing, values represent the mean of Ground corn samples were split using a riffle splitter to produce 2, 100 g samples. A dispersing agent (0.5 g of powdered synthetic amorphous silicon dioxide) was added to one of the samples. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Table 6. Chemical analysis of diets, Exp. 1, (as-fed basis) Phase 1 Phase 2 3 4 Item, % Conventional Enogen Feed corn Conventional Enogen Feed corn Dry matter 90.32 90.78 89.41 89.42 Crude protein 20.95 20.55 20.35 19.85 Acid detergent fiber 3.10 3.00 3.10 3.30 Neutral detergent fiber 6.50 6.80 5.70 6.60 Ca 0.76 0.81 0.73 0.78 P 0.54 0.52 0.48 0.51 Feed samples were collected approximately 2 days after each feed delivery, pooled within corn source for each phase, and analyzed. (Ward Laboratories, Inc., Kearney, NE). The experimental diets were fed in 2 phases: d 0 to 14, and d 14 to 35. Yellow dent corn. Enogen Feed corn, Syngenta Seeds, LLC, Downers Grove, IL. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Table 7. Chemical analysis of diets, Exp. 2, (as-fed basis) Phase 1 Phase 2 Phase 3 Conventiona 3 4 Item, % Conventional Enogen Feed corn l Enogen Feed corn Conventional Enogen Feed corn Dry matter 89.15 88.62 88.22 88.53 88.04 88.69 Crude protein 17.25 16.3 14.35 13.65 12.3 12.6 Acid detergent fiber 2.55 2.75 2.65 2.45 2.15 2.35 Neutral detergent fiber 5.55 5.65 4.90 5.75 5.25 5.65 Ca 0.72 0.73 0.76 0.71 0.59 0.59 P 0.42 0.40 0.37 0.34 0.33 0.33 Feed samples were collected approximately 2 days after each fe ed delivery, pooled within corn source fo r each phase, and analyzed. (Ward Laboratories, Inc., Kearney, NE). The experimental diets were fed in 3 phases: d 0 to 14, d 14 to 42, and d 42 to 83. Yellow dent corn. Enogen Feed corn, Syngenta Seeds, LLC, Downers Grove, IL. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Table 8. Effects of corn source and particle size on growth performance of weanling pigs, Exp. 1 2 3 Conventional, µm Enogen Feed corn, µm Probability, P = Source × particle size, Source × particle size, Item 300 600 900 300 600 900 SEM linear quadratic BW , kg d 0 6.6 6.6 6.6 6.6 6.6 6.6 0.05 0.754 0.856 d 14 11.7 11.7 11.7 12.1 12.3 12.0 0.20 0.911 0.467 d 35 23.9 23.8 23.5 23.9 24.2 23.3 0.33 0.749 0.283 d 0 to 14 ADG , g 366 363 356 394 405 386 12.3 0.939 0.497 ADFI, g 448 440 440 459 483 482 14.4 0.237 0.472 G:F, g/kg 818 825 810 860 841 800 11.6 0.027 0.945 d 14 to 35 ADG, g 571 577 562 560 569 540 9.4 0.530 0.584 ADFI, g 907 898 900 882 939 902 19.3 0.408 0.071 G:F, g/kg 631 644 625 636 607 600 9.8 0.095 0.125 d 0 to 35 ADG, g 489 490 477 494 503 478 8.9 0.801 0.462 Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 ADFI , g 722 712 711 713 757 734 15.3 0.203 0.086 G:F , g/kg 677 689 672 693 666 652 8.0 0.027 0.127 A total of 360 pigs (Line 200 × 400, DNA, Columbus, NE initially 6.6 kg) with 12 pens per treatment and 5 pigs per pen. Yellow dent corn. Enogen Feed corn, Syngenta Seeds, LLC, Downers Grove, IL. BW = body weight. The experimental diets were fed in 2 phases: d 0 to 14, and d 14 to 35. ADG = average daily gain; ADFI = average daily feed intake; G:F = gain to feed. Conventional yellow dent corn when particle size of ground corn was reduced (linear, P = 0.640), Enogen Feed corn when particle size was reduced (quadratic, P = 0.030). Convetional yellow dent corn when particle size of ground corn was reduced (linear, P = 0.610), Enogen Feed corn when particle size was reduced (linear, P = <0.001). Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Table 9. Main effects of corn source and particle size on growth performance of weanling pigs, Exp. 1 Source Particle size, µm Probability, P = 2 3 Conventional Enogen Feed corn SEM Probability, P = 300 600 900 SEM Linear Quadratic Item BW , kg d 0 6.6 6.6 0.05 0.778 6.6 6.6 6.6 0.05 0.531 0.612 d 14 11.7 12.1 0.15 0.001 11.9 12.0 11.8 0.17 0.498 0.394 d 35 23.7 23.8 0.25 0.731 23.9 24.0 23.4 0.27 0.091 0.131 d 0 to 14 ADG , g 362 395 7.9 0.001 380 384 371 9.2 0.424 0.391 ADFI, g 442 475 10.0 0.003 453 461 461 11.3 0.563 0.712 G:F, g/kg 818 834 6.7 0.094 839 833 805 8.2 0.006 0.268 d 14 to 35 ADG, g 570 556 6.2 0.063 566 573 551 7.1 0.101 0.064 ADFI, g 902 908 11.4 0.641 895 919 901 15.3 0.708 0.160 G:F, g/kg 633 614 5.9 0.016 634 626 613 6.7 0.036 0.757 d 0 to 35 Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 ADG, g 485 492 6.3 0.309 491 497 478 7.1 0.090 0.074 ADFI, g 715 734 11.3 0.062 718 734 723 12.5 0.681 0.190 G:F, g/kg 679 671 4.6 0.143 685 678 662 5.7 0.005 0.573 A total of 360 pigs (Line 200 × 400, DNA, Columbus, NE, initially 6.6 kg) with 12 pens per treatment and 5 pigs per pen. Yellow dent corn. Enogen Feed corn, Syngenta Seeds, LLC, Downers Grove, IL. BW = body weight. The experimental diets were fed in 2 phases: d 0 to 14, and d 14 to 35. ADG = average daily gain; ADFI = average daily feed intake; G:F = gain to feed. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Table 10. Effects of corn source and particle size on growth performance and carcass characteristics of finishing pigs, Exp. 2 2 3 Conventional, µm Enogen Feed corn, µm Probability, P = Source × particle size, Source × particle size, Item 300 600 900 300 600 900 SEM linear quadratic BW , kg d 0 50.6 50.3 49.8 49.8 48.7 49.7 1.00 0.554 0.228 d 28 78.9 78.6 77.8 78.1 78.3 77.9 0.31 0.314 0.339 d 56 107.1 106.4 104.9 106.6 107.4 105.4 0.63 0.380 0.849 d 83 134.7 132.0 133.1 135.4 134.2 132.1 1.33 0.669 0.600 d 0 to 28 ADG , g 1,040 1,034 1,006 1,012 1,019 1,004 16.8 0.388 0.883 ADFI, g 2,217 2,250 2,294 2,199 2,323 2,288 41.4 0.731 0.484 G:F, g/kg 468 460 439 460 440 439 6.6 0.681 0.250 d 28 to 56 ADG, g 1,004 994 967 1,020 1,039 981 21.2 0.960 0.366 ADFI, g 2,886 2,950 2,948 2,946 2,984 3,025 44.7 0.837 0.622 G:F, g/kg 348 337 328 346 348 324 5.2 0.821 0.140 Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 d 56 to 83 ADG, g 969 939 945 983 973 941 22.9 0.645 0.397 ADFI, g 2,965 2,965 3,079 3,048 3,118 3,090 55.0 0.485 0.248 G:F, g/kg 327 317 308 322 312 305 5.3 0.913 0.915 d 0 to 83 ADG, g 1,006 990 974 1,006 1,011 976 11.7 0.922 0.327 ADFI, g 2,682 2,714 2,766 2,719 2,799 2,793 34.3 0.957 0.491 G:F, g/kg 375 365 352 370 361 350 3.4 0.895 0.801 Carcass characteristics HCW , kg 100.0 98.1 97.8 100.7 99.7 97.6 1.70 0.754 0.600 0.00 Carcass yield, % 74.1 74.2 73.6 74.3 74.1 73.8 2 0.960 0.259 0.49 Backfat depth, mm 16.3 16.3 16.4 16.2 16.4 16.5 8 0.871 0.930 0.93 Loin depth, mm 67.4 65.2 65.1 66.0 65.1 66.4 7 0.171 0.989 0.29 Lean, % 54.8 54.4 54.4 54.6 54.4 54.6 5 0.519 0.891 A total of 323 mixed gender pigs (Line 241 × 600, DNA, Columbus, NE, initially 50.0 ± 0.3 kg) were used with 9 pigs per pen and 6 pens per Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 treatment. Conventional yellow dent. Enogen Feed corn, Syngenta Seeds, LLC, Downers Grove, IL. BW = body weight. ADG = average daily gain; ADFI = average daily feed intake; G:F = gain to feed. HCW = hot carcass weight. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Table 11. Main effects of corn source and particle size on growth performance and carcass characteristics of finishing pigs, Exp. 2 Source Particle size, µm Probability, P = Enogen Probability, P Conventional SEM 300 600 900 SEM Linear Quadratic Item Feed corn = BW , kg d 0 50.2 49.4 0.90 0.091 50.2 49.5 49.8 0.93 0.457 0.324 d 28 78.9 77.7 1.04 0.064 78.9 78.1 77.9 1.09 0.164 0.702 d 56 106.6 106.1 1.01 0.494 107.2 106.6 105.1 1.08 0.031 0.564 d 83 133.6 133.5 1.17 0.933 135.4 132.8 132.6 1.32 0.057 0.328 d 0 to 28 ADG , g 1,028 1,010 10.6 0.185 1,027 1,025 1,005 12.7 0.187 0.497 ADFI, g 2,266 2,260 32.8 0.875 2,220 2,279 2,290 38.2 0.133 0.541 G:F, g/kg 454 448 5.5 0.231 462 451 439 6.2 0.002 0.929 d 28 to 56 ADG, g 988 1014 13.1 0.128 1,012 1,017 974 15.6 0.067 0.177 Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 ADFI, g 2,929 2,984 25.9 0.131 2,917 2,966 2,987 32.0 0.125 0.702 G:F, g/kg 338 340 3.0 0.615 347 343 326 3.7 0.001 0.166 d 56 to 83 ADG, g 949 967 13.4 0.349 975 957 943 16.7 0.187 0.932 ADFI, g 3,001 3,086 31.8 0.064 3,004 3,043 3,085 39.4 0.151 0.975 G:F, g/kg 317 313 3.1 0.512 324 315 306 3.9 0.003 0.979 d 0 to 83 ADG, g 990 998 6.7 0.398 1,005 1,001 975 8.3 0.014 0.289 ADFI, g 2,724 2,767 20.4 0.145 2,701 2,756 2,779 39.4 0.043 0.691 G:F, g/kg 364 360 3.1 0.368 372 363 350 3.9 0.001 0.419 Carcass characteristics HCW , kg 98.6 99.3 1.233 0.576 100.3 98.9 97.7 1.355 0.093 0.919 Carcass yield, % 74.0 74.1 0.001 0.437 74.2 74.1 73.7 0.002 0.023 0.253 Backfat depth, mm 16.4 16.3 0.304 0.965 16.5 16.4 16.2 0.357 0.645 0.949 Loin depth, mm 65.9 65.8 0.615 0.921 66.7 65.7 65.2 0.728 0.317 0.193 Lean, % 54.5 54.5 0.175 0.955 54.7 54.4 54.5 0.213 0.509 0.482 A total of 323 mixed gender pigs (Line 241 × 600, DNA, Columbus, NE, initially 50.0 ± 0.3 kg) with 9 pigs per pen and 6 pens per treatment. Yellow dent corn. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Enogen Feed corn, Syngenta Seeds, LLC, Downers Grove, IL. BW = body weight. ADG = average daily gain; ADFI = average daily gain; G:F = gain to feed. HCW = hot carcass weight. Accepted Manuscript Downloaded from https://academic.oup.com/tas/advance-article/doi/10.1093/tas/txab120/6322567 by DeepDyve user on 20 July 2021 Table 12. Effects of particle size on stomach ulceration and keratinization for Exp. 2 2 3 Conventional Enogen Feed corn Probability Source × particle Item 300 600 900 300 600 900 size Source Particle size Ulcer score 1.42 1.42 1.67 1.92 1.50 1.42 0.178 0.438 0.840 Keratinization score 2.92 2.00 1.58 1.42 1.92 1.17 0.055 0.002 0.015 On d 79, 2 pigs per pen, 1 barrow and 1 gilt of equal weight, were selected and transported to Natural Food Holdings, Sioux Center, Iowa to collect stomachs. The stomachs were taken to the Kansas State University diagnostic lab where a scoring system was used to determine the severity of ulceration and keratinization of the esophageal opening of the stomach. Yellow dent corn. Enogen Feed corn, Syngenta Seeds, LLC, Downers Grove, IL. Stomachs were scored on a scale of 1 to 4 with 1 = no ulceration, 2 = <25% ulceration, 3 = 25-75% ulceration and 4 > 75%. This scoring criteria used was the same for keratinization. Accepted Manuscript

Journal

Translational Animal Science – Oxford University Press

Published: Jul 16, 2021

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