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PINK BOLLWORM MORTALITY AND CRY1AC TOXIC PROTEIN IN NUCOTN 33B (BT) COTTON BOLLS COMPARED WITH DPL 5415 COTTON BOLLS, 2001

PINK BOLLWORM MORTALITY AND CRY1AC TOXIC PROTEIN IN NUCOTN 33B (BT) COTTON BOLLS COMPARED WITH... (M6) COTTON: Gossypium hirsutum L., DPL 5415, NuCOTN 33B (Bt) PINK BOLLWORM MORTALITY AND CRY1AC TOXIC PROTEIN IN NUCOTN 33B (BT) COTTON BOLLS COMPARED WITH DPL 5415 COTTON BOLLS, 2001 T. J. Henneberry, L. Forlow Jech, T. de la Torre, and J. Maurer Western Cotton Research Laboratory USDA-ARS, PWA 4135 E. Broadway Road Phoenix, AZ 85040-8803 Phone: (602) 437-0121 Fax: (602) 437-1274 E-mail: thenneberry@wcrl.ars.usda.gov Pink bollworm (PBW): Pectinophora gossypiella (Saunders) Significant reductions in Cry1Ac insect toxic protein levels in transgenic cottons have been reported with increasing plant age after planting. We investigated PBW larval mortality on cotton plants in early-, mid-, and late season in relation to Cry1Ac levels occurring in 21-day-old Bt and DPL 5415 cotton bolls. DPL 5415 and Bt cotton seeds were planted on 19 Apr 2001 in 7 rows wide by 60 ft long plots at the Western Cotton Research Laboratory, Phoenix, AZ. Plots were arranged in an RCB design with four replications. Plots were furrow irrigated every 14 days. Last irrigations occurred on 21 Sept and plots were defoliated on 1 Nov. The amounts of Cry1Ac protein in Bt cotton bolls were determined on days 83, 118, and 152 after planting to determine amounts of the material in aging cotton plants in relation to PBW larval mortalities feeding on the bolls. Materials, sample preparations, solutions, extractions, dilutions, and assays were exactly as described in the Envirologix, Inc., Cry1Ab/Cry1Ac plate kit (Envirologix, Portland, ME). Samples were 0.6-cm boll pieces that were weighed and placed in 1.5-cm microcentrifuge tubes. Boll pieces were homogenized in extraction buffer with a fitted pestle. ANOVAs were conducted for all data and contingent upon a significant F test; means were separated using LSD method (P ≤ 0.05). Total numbers of larvae found in DPL 5415 and Bt bolls were variable, but no live larvae were found in Bt bolls compared with 3.2-52% live larvae per boll found in DPL 5415 bolls. No larvae developed beyond the first instar in Bt bolls compared with 0.06 to 0.08, 0.01 to 0.04, and 0.01 to 0.45 live second, third, and fourth instar larvae, respectively, per boll found in DPL 5415 bolls. Amounts of Cry1Ac toxic protein found in 21-day-old cotton bolls decreased with plant age. PBW larval mortality was 100% in bolls at all Cry1Ac levels measured using the ELISA procedures. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Arthropod Management Tests Oxford University Press

PINK BOLLWORM MORTALITY AND CRY1AC TOXIC PROTEIN IN NUCOTN 33B (BT) COTTON BOLLS COMPARED WITH DPL 5415 COTTON BOLLS, 2001

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Publisher
Oxford University Press
Copyright
© Published by Oxford University Press.
eISSN
2155-9856
DOI
10.1093/amt/27.1.M6
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Abstract

(M6) COTTON: Gossypium hirsutum L., DPL 5415, NuCOTN 33B (Bt) PINK BOLLWORM MORTALITY AND CRY1AC TOXIC PROTEIN IN NUCOTN 33B (BT) COTTON BOLLS COMPARED WITH DPL 5415 COTTON BOLLS, 2001 T. J. Henneberry, L. Forlow Jech, T. de la Torre, and J. Maurer Western Cotton Research Laboratory USDA-ARS, PWA 4135 E. Broadway Road Phoenix, AZ 85040-8803 Phone: (602) 437-0121 Fax: (602) 437-1274 E-mail: thenneberry@wcrl.ars.usda.gov Pink bollworm (PBW): Pectinophora gossypiella (Saunders) Significant reductions in Cry1Ac insect toxic protein levels in transgenic cottons have been reported with increasing plant age after planting. We investigated PBW larval mortality on cotton plants in early-, mid-, and late season in relation to Cry1Ac levels occurring in 21-day-old Bt and DPL 5415 cotton bolls. DPL 5415 and Bt cotton seeds were planted on 19 Apr 2001 in 7 rows wide by 60 ft long plots at the Western Cotton Research Laboratory, Phoenix, AZ. Plots were arranged in an RCB design with four replications. Plots were furrow irrigated every 14 days. Last irrigations occurred on 21 Sept and plots were defoliated on 1 Nov. The amounts of Cry1Ac protein in Bt cotton bolls were determined on days 83, 118, and 152 after planting to determine amounts of the material in aging cotton plants in relation to PBW larval mortalities feeding on the bolls. Materials, sample preparations, solutions, extractions, dilutions, and assays were exactly as described in the Envirologix, Inc., Cry1Ab/Cry1Ac plate kit (Envirologix, Portland, ME). Samples were 0.6-cm boll pieces that were weighed and placed in 1.5-cm microcentrifuge tubes. Boll pieces were homogenized in extraction buffer with a fitted pestle. ANOVAs were conducted for all data and contingent upon a significant F test; means were separated using LSD method (P ≤ 0.05). Total numbers of larvae found in DPL 5415 and Bt bolls were variable, but no live larvae were found in Bt bolls compared with 3.2-52% live larvae per boll found in DPL 5415 bolls. No larvae developed beyond the first instar in Bt bolls compared with 0.06 to 0.08, 0.01 to 0.04, and 0.01 to 0.45 live second, third, and fourth instar larvae, respectively, per boll found in DPL 5415 bolls. Amounts of Cry1Ac toxic protein found in 21-day-old cotton bolls decreased with plant age. PBW larval mortality was 100% in bolls at all Cry1Ac levels measured using the ELISA procedures.

Journal

Arthropod Management TestsOxford University Press

Published: Jan 1, 2002

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