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‘NUCOTN 33B’ (BT) AND ‘DELTAPINE (DPL) 5415’ COTTONS: PINK BOLLWORM OVIPOSITION AND MATING, 2003

‘NUCOTN 33B’ (BT) AND ‘DELTAPINE (DPL) 5415’ COTTONS: PINK BOLLWORM OVIPOSITION AND MATING, 2003 (M2) COTTON: Gossypium hirsutum L., 'Deltapine 5415', 'NuCOTN 33B' 'NUCOTN 33B' (BT) AND 'DELTAPINE (DPL) 5415' COTTONS: PINK BOLLWORM OVIPOSITION AND MATING, 2003 T. J. Henneberry USDA-ARS, Western Cotton Research Laboratory 4135 E. Broadway Road Phoenix, AZ 85040-8803 Phone: (602) 437-0121 Fax: (602) 437-1274 E-mail: thenneberry@wcrl.ars.usda.gov L. Forlow Jech T. de la Torre Pink bollworm (PBW): Pectinophora gossypiella (Saunders) We conducted preliminary laboratory studies to investigate oviposition of non-Bt resistant PBW in choice and no-choice tests with 'NuCOTN 33B' (Bt) and 'Deltapine (DPL) 5415' bolls. All cottons used in the studies were grown in pots under greenhouse conditions at Phoenix, AZ. Cotton flowers were tagged to obtain known age bolls. PBW moths were from the Western Cotton Research Laboratory colony that has been reared on artificial diet for more than 250 generations. Mating-oviposition containers were paraffin- coated cardboard 8-oz cylindrical containers. Container lids had 2.5-cm screen covered holes in the center for ventilation. Lids also had approximately 0.63-cm holes that accommodated small vials containing 10% sugar solutions for adult food. To obtain known age and gender moths, PBW larvae in the last larval instar were sexed and 300 males and 300 females were placed in separate 1.0-oz containers with strips of artificial diet until pupation. Pupae were collected and held to moth emergence. Experiments were conducted using RCB designs with 12 to 14 replications in 26.7°C controlled temperature cabinets. For all experiments, data were analyzed using ANOVA and means separated using F protected LSD (P £ 0.05). Some comparisons between Bt and 'DPL 5415' boll differences were also made using Student t-tests (P £ 0.05). For experiment 1, a cultivar choice test, a 7-, 21- and 35-day-old harvested boll of each cultivar was paired with its same age counterpart in each of 50 mating-oviposition containers. Three pairs of 3- to 4-day-old moths were placed in each mating-oviposition container with the pairs of 7-, 21-, or 35-day old Bt and 'DPL 5415' bolls. After 48 h, containers were placed in a refrigerator and chilled moths were placed in 70% alcohol solutions. Eggs on bolls were counted using a stereomicroscope. All female moths were dissected and examined for the presence of spermatophores. For experiment 2, a boll age choice test, one boll of each age for each cultivar (7, 21, and 35 days) was placed in each individual container. Moth pairs, egg counting and dissections of females to detect and record spermatophores were as described for experiment one. Experiment 3 was a no choice test, with one Bt or one 'DPL 5415', 21 to 28-day-old boll placed individually in each of 10, 8- oz containers. Five three-day-old moth pairs were placed in each container. After 72 h, containers were placed in the refrigerator and moths after chilling were stored in 70% alcohol prior to dissection and examination for the presence of a spermataophore. Eggs on bolls were counted with the aid of a stereomicroscope. Data were analyzed using Student's t-tests and presented in the text only. For experiment one, mated PBW female moths in the choice test laid similar numbers of eggs on 'DPL 5415' and Bt cotton bolls of all ages tested (Table 1). Boll age also had no effect on average PBW mating percentages. Percentages of female moths mated were 72.0 ± 5.0, 73.3 ± 4.7, and 70.7 ± 5.2 in mating- oviposition containers with 7-, 21-, or 35-day-old bolls, respectively. For experiment 2, there were no significant differences in oviposition for cultivar or boll age (Table 2). Also, 94.4% and 100.0% of the female moths confined with Bt and 'DPL 5415' bolls, respectively, mated at least one time. For experiment 3, female mating percentages in containers with Bt or 'DPL 5415' bolls were 84 and 86%, respectively. The difference was not significantly different . Numbers of eggs laid on Bt bolls averaged 27 and 22 per female on 'DPL 5415' bolls. The difference was not significantly different. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Arthropod Management Tests Oxford University Press

‘NUCOTN 33B’ (BT) AND ‘DELTAPINE (DPL) 5415’ COTTONS: PINK BOLLWORM OVIPOSITION AND MATING, 2003

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Oxford University Press
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© Published by Oxford University Press.
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2155-9856
DOI
10.1093/amt/29.1.M2
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Abstract

(M2) COTTON: Gossypium hirsutum L., 'Deltapine 5415', 'NuCOTN 33B' 'NUCOTN 33B' (BT) AND 'DELTAPINE (DPL) 5415' COTTONS: PINK BOLLWORM OVIPOSITION AND MATING, 2003 T. J. Henneberry USDA-ARS, Western Cotton Research Laboratory 4135 E. Broadway Road Phoenix, AZ 85040-8803 Phone: (602) 437-0121 Fax: (602) 437-1274 E-mail: thenneberry@wcrl.ars.usda.gov L. Forlow Jech T. de la Torre Pink bollworm (PBW): Pectinophora gossypiella (Saunders) We conducted preliminary laboratory studies to investigate oviposition of non-Bt resistant PBW in choice and no-choice tests with 'NuCOTN 33B' (Bt) and 'Deltapine (DPL) 5415' bolls. All cottons used in the studies were grown in pots under greenhouse conditions at Phoenix, AZ. Cotton flowers were tagged to obtain known age bolls. PBW moths were from the Western Cotton Research Laboratory colony that has been reared on artificial diet for more than 250 generations. Mating-oviposition containers were paraffin- coated cardboard 8-oz cylindrical containers. Container lids had 2.5-cm screen covered holes in the center for ventilation. Lids also had approximately 0.63-cm holes that accommodated small vials containing 10% sugar solutions for adult food. To obtain known age and gender moths, PBW larvae in the last larval instar were sexed and 300 males and 300 females were placed in separate 1.0-oz containers with strips of artificial diet until pupation. Pupae were collected and held to moth emergence. Experiments were conducted using RCB designs with 12 to 14 replications in 26.7°C controlled temperature cabinets. For all experiments, data were analyzed using ANOVA and means separated using F protected LSD (P £ 0.05). Some comparisons between Bt and 'DPL 5415' boll differences were also made using Student t-tests (P £ 0.05). For experiment 1, a cultivar choice test, a 7-, 21- and 35-day-old harvested boll of each cultivar was paired with its same age counterpart in each of 50 mating-oviposition containers. Three pairs of 3- to 4-day-old moths were placed in each mating-oviposition container with the pairs of 7-, 21-, or 35-day old Bt and 'DPL 5415' bolls. After 48 h, containers were placed in a refrigerator and chilled moths were placed in 70% alcohol solutions. Eggs on bolls were counted using a stereomicroscope. All female moths were dissected and examined for the presence of spermatophores. For experiment 2, a boll age choice test, one boll of each age for each cultivar (7, 21, and 35 days) was placed in each individual container. Moth pairs, egg counting and dissections of females to detect and record spermatophores were as described for experiment one. Experiment 3 was a no choice test, with one Bt or one 'DPL 5415', 21 to 28-day-old boll placed individually in each of 10, 8- oz containers. Five three-day-old moth pairs were placed in each container. After 72 h, containers were placed in the refrigerator and moths after chilling were stored in 70% alcohol prior to dissection and examination for the presence of a spermataophore. Eggs on bolls were counted with the aid of a stereomicroscope. Data were analyzed using Student's t-tests and presented in the text only. For experiment one, mated PBW female moths in the choice test laid similar numbers of eggs on 'DPL 5415' and Bt cotton bolls of all ages tested (Table 1). Boll age also had no effect on average PBW mating percentages. Percentages of female moths mated were 72.0 ± 5.0, 73.3 ± 4.7, and 70.7 ± 5.2 in mating- oviposition containers with 7-, 21-, or 35-day-old bolls, respectively. For experiment 2, there were no significant differences in oviposition for cultivar or boll age (Table 2). Also, 94.4% and 100.0% of the female moths confined with Bt and 'DPL 5415' bolls, respectively, mated at least one time. For experiment 3, female mating percentages in containers with Bt or 'DPL 5415' bolls were 84 and 86%, respectively. The difference was not significantly different . Numbers of eggs laid on Bt bolls averaged 27 and 22 per female on 'DPL 5415' bolls. The difference was not significantly different.

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Arthropod Management Tests – Oxford University Press

Published: Jan 1, 2004

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