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Arthropod Management Tests, 2016, 1–2 doi: 10.1093/amt/tsv181 (G10) CHRYSANTHEMUM: Chrysanthemum indicum (L.), “Venus Purple,” “Eureka Yellow” Insecticidal Control of the American Serpentine Leafminer, 2014* 1,2 3 4 5 James Sanchez, O. Milo Lewis, Peter Krauter, Carlos Bogran, and Kevin M. Heinz 1 2 2475 TAMU, College Station, TX 77843, Phone: (979) 862-3407 (james2793@tamu.edu), Corresponding author, e-mail: 3 4 5 james2793@tamu.edu, E-mail: milo.lewis@tamu.edu, E-mail: p-krauter@tamu.edu, P.O. Box 51230, Mainland, PA 19451 (cbogran@ohp.com), and TAMU 2475, College Station, TX 77845, Phone: (979) 862-3407 (kmheinz@tamu.edu) Subject Editor: Carlos Bogran American Serpentine Leafminer; Liriomyza trifoli (Burgess) L. trifolii damages several ornamental and vegetable crops by feed- Treatments were assigned to experimental units (single plants) ing and ovipositing on leaves. Larval development by L. trifolii lar- under a RCB design with 10 replicates (Table 1). Each block con- vae within the leaves creates significant crop injury and results in sisted of three infested plants with approximately the same number characteristic “mines” on infested foliage. The objective of this of mines per plant. Insecticide treatments were applied using a study was to assess efficacy of spirotetramat and azadirachtin 750 ml hand-held sprayer; treated plants were laid on their sides on against leafminer larvae infesting Chrysanthemum morifolium 18 inch plastic plant saucers to allow leafminer prepuae to fall freely Ramat. The experiment was conducted in a greenhouse at the Texas from the plants and facilitate collection. Fallen pupae were collected A&M Biological Control Facility in College Station, Texas. L. trifo- from the saucers, counted and placed into capped plastic vials. Vials lii used for this experiment were reared under laboratory conditions were held in the laboratory at room temperature until all emerged on bean plants (Phaseolus vulgaris L.) and Gerbera Daisies (Gerbera adults had died. Adult count data were analyzed in JMP Pro 11 jamesonii Bolus) supplemented with honey under a 14:10 (L:D) pho- (SAS Institute Inc., Cary, NC). Due to non-normally distributed re- toperiod. Chrysanthemums (a mix of cv. “Sunset Orange” and siduals, the data were subjected to the non-parametric Kruskal– “Calisto Pink”, Ball Seed, Chicago, IL) were grown from cuttings in Wallis test and means testing was carried out when appropriate us- Sunshine mix #1 soil (Sun Gro Horticulture, Vancouver, British ing the Wilcoxon Each pair test. Colombia, Canada) in four inch pots for 5 weeks, before the study While no differences were found in the number of L. trifolii pu- began. Test plants were exposed to approximately 800 L. trifolii pae collected (Kruskal–Wallis, P> 0.05), differences between treat- adults to allow oviposition for a period of 48 h then shaken to re- ments were found in the number of eclosed adults (Kruskal–Wallis, move the insects. Plants were then moved into a cage to exclude L. P< 0.05). Vials from Azatin O and Kontos treatments had al- trifolii adults and held for 7 days, until the majority of larvae were most half the number of adults than the check vials, indicating both second instars and infested plants were ready to be used in the insec- insecticides may be used to control leafminers in a commercial ticide test. setting. * This study was funded by industry gifts of pesticide funding. V C The Author 2016. Published by Oxford University Press on behalf of the Entomological Society of America. 1 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com 2 Arthropod Management Tests, 2016, Vol. 41, No. 1 Table 1 Treatment Rate per 100 gal. Method of application Mean pupae collected Mean adults eclosed Azatin O 12 oz. Spray 7.2 2.5a Kontos 3.4 oz. Spray 5.3 2.5a No Spray check n/a n/a 7.7 4.9b Kruskal–Wallis P value 0.1773 0.0381 Means followed by the same letter in the same column are not significantly different (Wilcoxon each pair, P> 0.05).
Arthropod Management Tests – Oxford University Press
Published: Jan 1, 2016
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