CONTROL OF TWOSPOTTED SPIDER MITE ON GREENHOUSE MARIGOLD WITH AZA-DIRECT AND MPEDE, 2012
CONTROL OF TWOSPOTTED SPIDER MITE ON GREENHOUSE MARIGOLD WITH AZA-DIRECT AND MPEDE, 2012
Price, James F.; Nagle, Curtis A.
2013-01-01 00:00:00
Arthropod Management Tests 2013, Vol. 38 doi: 10.4182/amt.2013.G13 (G13) MARIGOLD: Tagetes patula L., ‘Petite’ CONTROL OF TWOSPOTTED SPIDER MITE ON GREENHOUSE MARIGOLD WITH AZA-DIRECT AND M- PEDE, 2012 James F. Price University of Florida-IFAS Gulf Coast Research and Education Center 14625 CR 672 Wimauma, FL 33598 Phone: (813) 633-4123 Fax: (813) 634-0001 Email: jfprice@ufl.edu Curtis A. Nagle Email: cnagle@ufl.edu Twospotted spider mite (TSM): Tetranychus urticae Koch The efficacy of a single application of different rates of Aza-Direct, M-Pede and their combination was evaluated and compared to a non-treated check for twospotted spider mite control on greenhouse ornamentals at the Gulf Coast Research and Education Center, Wimauma, Fla. in Apr-May 2012. Two seeds of ‘Petite’, mixed colors marigold, per 2-inch cell, were planted into polystyrene trays on 13 Mar. The resulting seedlings were transplanted, one each, to 6-inch pots on 6 Apr. Each plant was pinched to four nodes (13 Apr) and infested with about 25 motile TSM from a laboratory colony (18 Apr). Plots consisted of three pots. Treatments were replicated six times in a RCB design. The plants were spaced so that those from different plots would not touch. Pre-treatment samples were taken on 23 Apr and treatments were applied 1 day later using a hand-held sprayer with a spray wand outfitted with a nozzle containing a 45° core and a no. 4 disc. The sprayer was pressurized by CO to 40 psi and calibrated to wet the leaf surfaces thoroughly without excessive run off. Sampling continued periodically from 1 through 21 DAT. Samples consisted of three leaves per plot (one from each plant) and were transported to the laboratory where the numbers of motile and egg forms of TSM on the lower leaf surfaces were recorded with the aid of a dissecting microscope. Distinctions could not be made between viable and non-viable eggs, thus total eggs were recorded. Data were transformed log (x+1) prior to ANOVA and means were separated by Fisher’s Protected LSD test (P≤0.05). Non-transformed means were reported. Average motile TSM densities ranged from 19.8 to 37.2 per 3 leaves 1 day before treatments were applied (Table 1). One DAT, all chemically treated plots possessed densities that were lower than those in the non-treated checks. By 7 and 10 DAT, the lowest motile TSM densities occurred with all treatments which contained the higher rate of M-Pede. By 14 DAT, the effects of treatments were less clear as motile densities in plots treated with Aza-Direct at 12 fl oz (lower rate), M-Pede at 256 fl oz (higher rate) or a combination which contained either of these, possessed the lowest motile TSM densities. By 21 DAT, plots treated with the higher rate of Aza-Direct, the lower rate of M-Pede or that combination possessed densities not different from the non-treated check (Table 1). There were no significant differences in TSM egg densities among any treatments until 3 DAT (Table 2). At that time least egg densities occurred with all chemical treatments except the combination of the low rates of Aza-Direct and M-Pede. By 21 DAT the lowest egg densities were observed in plots treated with the higher rate of M-Pede, the lower rate of Aza-Direct or a combination which contained either of these (Table 2). No phytotoxicity was observed. This research was supported by industry gift(s) of pesticide and/or research funding. 1 Arthropod Management Tests 2013, Vol. 38 doi: 10.4182/amt.2013.G13 Table 1. No. motile TSM per three leaves Treatment/ Rate amt Pre- /100 gal treatment 1 DAT 3 DAT 7 DAT 10 DAT 14 DAT 21 DAT Formulation Non-treated --- 24.5a 25.2a 37.8a 48.0a 73.7a 131.3a 169.7a Aza-Direct 12 fl oz 19.8a 3.0b 6.0bc 11.2ab 7.0cd 8.8e 34.5cd Aza-Direct 16 fl oz 32.7a 7.8b 5.0bc 17.2ab 63.3ab 83.3ab 124.8ab M-Pede 128 fl oz 23.3a 3.3b 1.8cd 7.8bc 13.7cd 38.3b-d 88.2a-c M-Pede 256 fl oz 21.7a 7.3b 2.5b-d 5.3b-d 3.7 de 10.3 de 40.3b-d Aza-Direct 12 fl oz + M-Pede 128 fl oz 24.8a 4.5b 7.7b 10.2bc 14.8cd 17.5c-e 71.8b-d Aza-Direct 16 fl oz + M-Pede 128 fl oz 24.7a 4.3b 1.8b-d 13.3bc 23.5bc 40.8bc 70.3a-c Aza-Direct 12 fl oz + M-Pede 256 fl oz 37.2a 2.5b 1.2cd 2.0 d 6.2 de 4.8e 20.7e Aza-Direct 16 fl oz + M-Pede 256 fl oz 34.5a 7.0b 0.5 d 4.5cd 0.7e 5.2e 23.3 de F 0.52 2.63 6.74 3.59 8.11 8.01 5.79 8,40 P-value 0.8374 0.0203 <0.0001 0.0032 <0.0001 <0.0001 <0.0001 Means within a column followed by the same letter are not significantly different by Fisher’s Protected LSD (P≤0.05). Data were transformed log (x+1) prior to ANOVA; non-transformed means were presented. A ‘+’ sign indicates the products were combined. Table 2. No. egg TSM per three leaves Treatment/ Rate amt Pre- Formulation /100 gal treatment 1 DAT 3 DAT 7 DAT 10 DAT 14 DAT 21 DAT Non-treated --- 45.5 a 34.2 a 136.8 a 173.0 a 117.2 a 158.2 a 434.7 a Aza-Direct 12 fl oz 65.3 a 15.3 a 19.2 bc 21.8 bc 15.5 cd 35.0 b-d 56.3 c-e Aza-Direct 16 fl oz 41.7 a 16.5 a 13.5 bc 93.8 ab 94.0 ab 82.2 ab 334.7 ab M-Pede 128 fl oz 13.3 a 17.2 a 15.5 bc 20.0 bc 45.5 a-c 83.8 a-c 161.3 b-d M-Pede 256 fl oz 39.7 a 8.5 a 8.5 c 5.7 d 10.3 cd 43.2 a-d 48.7 de Aza-Direct 12 fl oz + M-Pede 128 fl oz 68.7 a 19.2 a 44.0 b 29.0 b 28.8 b-d 68.0 a-d 116.7 c-e Aza-Direct 16 fl oz + M-Pede 128 fl oz 32.7 a 16.2 a 14.3 bc 32.3 bc 43.8 a-d 80.8 a-c 178.0 a-c Aza-Direct 12 fl oz + M-Pede 256 fl oz 35.3 a 34.7 a 14.7 bc 6.2 cd 14.0 d 16.2 d 73.2 e Aza-Direct 16 fl oz + M-Pede 256 fl oz 36.3 a 16.5 a 6.7 c 5.7 d 12.2 d 70.7 cd 59.8 e F 0.43 1.03 5.10 6.04 3.80 2.54 4.95 8,40 P-value 0.8989 0.4271 0.0002 <0.0001 0.0021 0.0244 0.0003 Means within a column followed by the same letter are not significantly different by Fisher’s Protected LSD (P≤0.05). Data were transformed log (x+1) prior to ANOVA; non-transformed means were presented. A ‘+’ sign indicates the products were combined.
http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.pngArthropod Management TestsOxford University Presshttp://www.deepdyve.com/lp/oxford-university-press/control-of-twospotted-spider-mite-on-greenhouse-marigold-with-aza-ZRz936ku50
CONTROL OF TWOSPOTTED SPIDER MITE ON GREENHOUSE MARIGOLD WITH AZA-DIRECT AND MPEDE, 2012
Arthropod Management Tests 2013, Vol. 38 doi: 10.4182/amt.2013.G13 (G13) MARIGOLD: Tagetes patula L., ‘Petite’ CONTROL OF TWOSPOTTED SPIDER MITE ON GREENHOUSE MARIGOLD WITH AZA-DIRECT AND M- PEDE, 2012 James F. Price University of Florida-IFAS Gulf Coast Research and Education Center 14625 CR 672 Wimauma, FL 33598 Phone: (813) 633-4123 Fax: (813) 634-0001 Email: jfprice@ufl.edu Curtis A. Nagle Email: cnagle@ufl.edu Twospotted spider mite (TSM): Tetranychus urticae Koch The efficacy of a single application of different rates of Aza-Direct, M-Pede and their combination was evaluated and compared to a non-treated check for twospotted spider mite control on greenhouse ornamentals at the Gulf Coast Research and Education Center, Wimauma, Fla. in Apr-May 2012. Two seeds of ‘Petite’, mixed colors marigold, per 2-inch cell, were planted into polystyrene trays on 13 Mar. The resulting seedlings were transplanted, one each, to 6-inch pots on 6 Apr. Each plant was pinched to four nodes (13 Apr) and infested with about 25 motile TSM from a laboratory colony (18 Apr). Plots consisted of three pots. Treatments were replicated six times in a RCB design. The plants were spaced so that those from different plots would not touch. Pre-treatment samples were taken on 23 Apr and treatments were applied 1 day later using a hand-held sprayer with a spray wand outfitted with a nozzle containing a 45° core and a no. 4 disc. The sprayer was pressurized by CO to 40 psi and calibrated to wet the leaf surfaces thoroughly without excessive run off. Sampling continued periodically from 1 through 21 DAT. Samples consisted of three leaves per plot (one from each plant) and were transported to the laboratory where the numbers of motile and egg forms of TSM on the lower leaf surfaces were recorded with the aid of a dissecting microscope. Distinctions could not be made between viable and non-viable eggs, thus total eggs were recorded. Data were transformed log (x+1) prior to ANOVA and means were separated by Fisher’s Protected LSD test (P≤0.05). Non-transformed means were reported. Average motile TSM densities ranged from 19.8 to 37.2 per 3 leaves 1 day before treatments were applied (Table 1). One DAT, all chemically treated plots possessed densities that were lower than those in the non-treated checks. By 7 and 10 DAT, the lowest motile TSM densities occurred with all treatments which contained the higher rate of M-Pede. By 14 DAT, the effects of treatments were less clear as motile densities in plots treated with Aza-Direct at 12 fl oz (lower rate), M-Pede at 256 fl oz (higher rate) or a combination which contained either of these, possessed the lowest motile TSM densities. By 21 DAT, plots treated with the higher rate of Aza-Direct, the lower rate of M-Pede or that combination possessed densities not different from the non-treated check (Table 1). There were no significant differences in TSM egg densities among any treatments until 3 DAT (Table 2). At that time least egg densities occurred with all chemical treatments except the combination of the low rates of Aza-Direct and M-Pede. By 21 DAT the lowest egg densities were observed in plots treated with the higher rate of M-Pede, the lower rate of Aza-Direct or a combination which contained either of these (Table 2). No phytotoxicity was observed. This research was supported by industry gift(s) of pesticide and/or research funding. 1 Arthropod Management Tests 2013, Vol. 38 doi: 10.4182/amt.2013.G13 Table 1. No. motile TSM per three leaves Treatment/ Rate amt Pre- /100 gal treatment 1 DAT 3 DAT 7 DAT 10 DAT 14 DAT 21 DAT Formulation Non-treated --- 24.5a 25.2a 37.8a 48.0a 73.7a 131.3a 169.7a Aza-Direct 12 fl oz 19.8a 3.0b 6.0bc 11.2ab 7.0cd 8.8e 34.5cd Aza-Direct 16 fl oz 32.7a 7.8b 5.0bc 17.2ab 63.3ab 83.3ab 124.8ab M-Pede 128 fl oz 23.3a 3.3b 1.8cd 7.8bc 13.7cd 38.3b-d 88.2a-c M-Pede 256 fl oz 21.7a 7.3b 2.5b-d 5.3b-d 3.7 de 10.3 de 40.3b-d Aza-Direct 12 fl oz + M-Pede 128 fl oz 24.8a 4.5b 7.7b 10.2bc 14.8cd 17.5c-e 71.8b-d Aza-Direct 16 fl oz + M-Pede 128 fl oz 24.7a 4.3b 1.8b-d 13.3bc 23.5bc 40.8bc 70.3a-c Aza-Direct 12 fl oz + M-Pede 256 fl oz 37.2a 2.5b 1.2cd 2.0 d 6.2 de 4.8e 20.7e Aza-Direct 16 fl oz + M-Pede 256 fl oz 34.5a 7.0b 0.5 d 4.5cd 0.7e 5.2e 23.3 de F 0.52 2.63 6.74 3.59 8.11 8.01 5.79 8,40 P-value 0.8374 0.0203 <0.0001 0.0032 <0.0001 <0.0001 <0.0001 Means within a column followed by the same letter are not significantly different by Fisher’s Protected LSD (P≤0.05). Data were transformed log (x+1) prior to ANOVA; non-transformed means were presented. A ‘+’ sign indicates the products were combined. Table 2. No. egg TSM per three leaves Treatment/ Rate amt Pre- Formulation /100 gal treatment 1 DAT 3 DAT 7 DAT 10 DAT 14 DAT 21 DAT Non-treated --- 45.5 a 34.2 a 136.8 a 173.0 a 117.2 a 158.2 a 434.7 a Aza-Direct 12 fl oz 65.3 a 15.3 a 19.2 bc 21.8 bc 15.5 cd 35.0 b-d 56.3 c-e Aza-Direct 16 fl oz 41.7 a 16.5 a 13.5 bc 93.8 ab 94.0 ab 82.2 ab 334.7 ab M-Pede 128 fl oz 13.3 a 17.2 a 15.5 bc 20.0 bc 45.5 a-c 83.8 a-c 161.3 b-d M-Pede 256 fl oz 39.7 a 8.5 a 8.5 c 5.7 d 10.3 cd 43.2 a-d 48.7 de Aza-Direct 12 fl oz + M-Pede 128 fl oz 68.7 a 19.2 a 44.0 b 29.0 b 28.8 b-d 68.0 a-d 116.7 c-e Aza-Direct 16 fl oz + M-Pede 128 fl oz 32.7 a 16.2 a 14.3 bc 32.3 bc 43.8 a-d 80.8 a-c 178.0 a-c Aza-Direct 12 fl oz + M-Pede 256 fl oz 35.3 a 34.7 a 14.7 bc 6.2 cd 14.0 d 16.2 d 73.2 e Aza-Direct 16 fl oz + M-Pede 256 fl oz 36.3 a 16.5 a 6.7 c 5.7 d 12.2 d 70.7 cd 59.8 e F 0.43 1.03 5.10 6.04 3.80 2.54 4.95 8,40 P-value 0.8989 0.4271 0.0002 <0.0001 0.0021 0.0244 0.0003 Means within a column followed by the same letter are not significantly different by Fisher’s Protected LSD (P≤0.05). Data were transformed log (x+1) prior to ANOVA; non-transformed means were presented. A ‘+’ sign indicates the products were combined.
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