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ACELEPRYN AND DPX-HGW86 LIQUID APPLICATIONS FOR RESIDUAL SYSTEMIC BLACK CUTWORM CONTROL IN TURFGRASS, 2012

ACELEPRYN AND DPX-HGW86 LIQUID APPLICATIONS FOR RESIDUAL SYSTEMIC BLACK CUTWORM CONTROL IN... Arthropod Management Tests 2013, Vol. 38 doi: 10.4182/amt.2013.G1 (G1) BENTGRASS (CREEPING): Agrostis palustris Huds. ‘Penn G6’ ACELEPRYN AND DPX-HGW86 LIQUID APPLICATIONS FOR RESIDUAL SYSTEMIC BLACK CUTWORM CONTROL IN TURFGRASS, 2012 Eric J. Rebek Oklahoma State University Dept. of Entomology and Plant Pathology 127 Noble Research Center Stillwater, OK 74078-3033 Phone: (405) 744-4846 E-mail: eric.rebek@okstate.edu Black cutworm (BCW): Agrotis ipsilon (Hugnagel) This study was conducted in summer 2012 to evaluate residual activity of several systemic insecticides for control of black cutworm (Agrotis ipsilon) in creeping bentgrass. Treatments were applied to an experimental block of creeping bentgrass maintained under putting green standards and located at the Oklahoma State University Turfgrass Research Center in Stillwater, Oklahoma. Plots measured 3 ft by 3 ft (9 ft ) and were mowed at 0.155 inch and irrigated daily. Treatments consisted of the following insecticides: (1) Acelepryn (chlorantraniliprole) at 4.0 fl oz per acre; (2) Acelepryn at 8.0 fl oz per acre; (3) DPX-HGW86 20SC (cyantraniliprole) at 4.0 fl oz per acre; (4) DPX-HGW86 20SC at 8.0 fl oz per acre; (5) Acelepryn 1.67SC and DPX-HGW86 20SC at 4.0 fl oz per acre for each compound; (6) Meridian 25WG (thiamethoxam) at 17.0 oz per acre; and (7) Meridian 25WG and DPX-HGW86 20SC at 8.6 oz and 10.3 fl oz per acre, respectively. All treatments were compared against a non-treated check. All treatments and the non-treated check were replicated four times in a randomized complete block design. Treatments were applied mid-morning on May 8, 2012 with a CO -pressurized (18 psi) wheelbarrow sprayer equipped with two TX8008 flat fan nozzles and calibrated to deliver 2.0 gal/1000 ft . All plots were irrigated with approximately 1/10 inch of water following insecticide application. Each plot was infested artificially with 50 BCW eggs purchased from Benzon Research, Inc. (Carlisle, PA) on four occasions: May 22, June 18, July 23, and August 20, 2012. Eggs were placed into cages consisting of PVC cylinders measuring 8 inches in diameter. The bottom edge of each cylinder was beveled to facilitate driving them into the ground. Approximately 6 inches of each cage were exposed above ground. Each cage was fastened with a fine-mesh screen to allow for adequate light penetration and ventilation and to prevent bird predation on BCW larvae. Cages were rotated to a new plot quadrant for each infestation date so larvae were never exposed to previously infested turfgrass. Larvae were left in cages for a period of 2 weeks following infestation at which time larval counts were determined by pouring a solution of flushing agent (2 tbsp lemon-scented dish detergent per gal water) into each cylinder. Larval sampling occurred on June 5 (28 DAT), July 3 (56 DAT), August 6 (90 DAT), and September 4 (119 DAT) of 2012. Many plots had zero BCW larvae during sampling at 28 DAT and 56 DAT. Thus, data were not normally distributed and could not be analyzed with parametric statistics. Nonparametric Wilcoxon rank sum tests (PROC NPAR1WAY, SAS 9.2) were used to determine global differences among means for each sample date. Pairwise comparisons of treatments were analyzed using Kruskal- Wallis chi-square tests (PROC NPAR1WAY, SAS 9.2). Differences among treatments were highly significant at 28 and 56 DAT (P ≤ 0.0005) (Table 1). With the exception of Meridian 25WG, all treatments were significantly different from the non-treated check and no live BCW larvae were recovered from those plots at 28 DAT. Therefore, 100% control was achieved 4 weeks following treatment with DPX- HGW86 20SC, Acelepryn 1.67SC, and combinations of these two compounds at the rates tested. Additionally, treating with a combination of Meridian 25WG and DPX-HGW86 20SC resulted in 100% control even though Meridian 25WG as a stand-alone treatment did not control BCW. At 56 DAT, only the Acelepryn 1.67SC treatments and the combination of Acelepryn 1.67SC and DPX-HGW86 20SC resulted in 100% control of BCW larvae. These treatments and the combination of Meridian 25WG and DPX-HGW86 20SC were significantly different from the non-treated check. In contrast, both DPX- HGW86 20SC treatments and the Meridian 25WG treatment were not significantly different from the non-treated check. At 90 and 119 DAT, no treatment differences were found (Table 1). Overall, Acelepryn 1.67SC and DPX-HGW86 20SC provided complete control of BCW at 28 DAT when applied at rates of 4 and 8 fl oz per acre or when tank mixed at 4 fl oz per acre each. Combining Meridian 25WG with DPX-HGW86 also provided complete control of BCW, although the latter appears to be the key compound responsible for controlling BCW 1 Arthropod Management Tests 2013, Vol. 38 doi: 10.4182/amt.2013.G1 because Meridian 25WG did not provide any control when applied as a stand-alone treatment. At 56 DAT, only Acelepryn 1.67SC applied at either rate provided complete control of BCW. Although the combination of Acelepryn 1.67SC and DPX-HGW86 20SC also provided complete control, the former appears to be the key compound responsible for controlling BCW because DPX-HGW86 20SC did not provide any control when applied as a stand-alone treatment. However, data from the combination of Meridian 25WG and DPX-HGW86 20SC suggest that higher application rates of the latter compound (i.e., 10.3 fl oz per acre) may be necessary to achieve some control of BCW. None of the treatments provided sufficient control of BCW at 90 DAT and later. Thus, two applications of Acelepryn 1.67SC at rates of 4 or 8 fl oz per acre may be needed under heavy insect pressure to protect bentgrass putting greens from BCW in Oklahoma. Phytotoxicity was not observed for any treatments during the trial. Table 1. Mean (±S.E.) BCW per Cage Treatment/formulation Rate (lb ai/acre) 28 DAT 56 DAT 90 DAT 119 DAT Non-treated check --- 15.5 (1.9)a 12.5 (2.2)ab 7.0 (1.4)a 14.5 (0.7)a Meridian 25WG 0.266 17.3 (1.3)a 7.5 (3.0)bc 8.8 (3.7)a 11.3 (1.9)a DPX-HGW86 20SC 0.052 0 (0)b 18.0 (1.9)a 6.0 (1.3)a 12.0 (2.9)a DPX-HGW86 20SC 0.104 0 (0)b 9.3 (2.9)bc 8.3 (1.9)a 13.5 (3.5)a Meridian 25WG + 0.134 0 (0)b 4.3 (1.3)c 7.8 (3.1)a 13.3 (2.8)a DPX-HGW86 20SC 0.134 Acelepryn 1.67SC 0.052 0 (0)b 0 (0)d 10.8 (2.3)a 15.3 (1.4)a Acelepryn 1.67SC 0.104 0 (0)b 0 (0)d 3.8 (2.4)a 15.3 (5.4)a Acelepryn 1.67SC + 0.052 0 (0)b 0 (0)d 12.8 (2.3)a 18.5 (2.1)a DPX-HGW86 20SC 0.052 Means within columns followed by the same letter are not significantly different (Kruskal-Wallis chi-square, P > 0.05). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Arthropod Management Tests Oxford University Press

ACELEPRYN AND DPX-HGW86 LIQUID APPLICATIONS FOR RESIDUAL SYSTEMIC BLACK CUTWORM CONTROL IN TURFGRASS, 2012

Rebek, Eric J.
Arthropod Management Tests , Volume 38 (1) – Jan 1, 2013
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Oxford University Press
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© Published by Oxford University Press.
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10.4182/amt.2013.G1
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Abstract

Arthropod Management Tests 2013, Vol. 38 doi: 10.4182/amt.2013.G1 (G1) BENTGRASS (CREEPING): Agrostis palustris Huds. ‘Penn G6’ ACELEPRYN AND DPX-HGW86 LIQUID APPLICATIONS FOR RESIDUAL SYSTEMIC BLACK CUTWORM CONTROL IN TURFGRASS, 2012 Eric J. Rebek Oklahoma State University Dept. of Entomology and Plant Pathology 127 Noble Research Center Stillwater, OK 74078-3033 Phone: (405) 744-4846 E-mail: eric.rebek@okstate.edu Black cutworm (BCW): Agrotis ipsilon (Hugnagel) This study was conducted in summer 2012 to evaluate residual activity of several systemic insecticides for control of black cutworm (Agrotis ipsilon) in creeping bentgrass. Treatments were applied to an experimental block of creeping bentgrass maintained under putting green standards and located at the Oklahoma State University Turfgrass Research Center in Stillwater, Oklahoma. Plots measured 3 ft by 3 ft (9 ft ) and were mowed at 0.155 inch and irrigated daily. Treatments consisted of the following insecticides: (1) Acelepryn (chlorantraniliprole) at 4.0 fl oz per acre; (2) Acelepryn at 8.0 fl oz per acre; (3) DPX-HGW86 20SC (cyantraniliprole) at 4.0 fl oz per acre; (4) DPX-HGW86 20SC at 8.0 fl oz per acre; (5) Acelepryn 1.67SC and DPX-HGW86 20SC at 4.0 fl oz per acre for each compound; (6) Meridian 25WG (thiamethoxam) at 17.0 oz per acre; and (7) Meridian 25WG and DPX-HGW86 20SC at 8.6 oz and 10.3 fl oz per acre, respectively. All treatments were compared against a non-treated check. All treatments and the non-treated check were replicated four times in a randomized complete block design. Treatments were applied mid-morning on May 8, 2012 with a CO -pressurized (18 psi) wheelbarrow sprayer equipped with two TX8008 flat fan nozzles and calibrated to deliver 2.0 gal/1000 ft . All plots were irrigated with approximately 1/10 inch of water following insecticide application. Each plot was infested artificially with 50 BCW eggs purchased from Benzon Research, Inc. (Carlisle, PA) on four occasions: May 22, June 18, July 23, and August 20, 2012. Eggs were placed into cages consisting of PVC cylinders measuring 8 inches in diameter. The bottom edge of each cylinder was beveled to facilitate driving them into the ground. Approximately 6 inches of each cage were exposed above ground. Each cage was fastened with a fine-mesh screen to allow for adequate light penetration and ventilation and to prevent bird predation on BCW larvae. Cages were rotated to a new plot quadrant for each infestation date so larvae were never exposed to previously infested turfgrass. Larvae were left in cages for a period of 2 weeks following infestation at which time larval counts were determined by pouring a solution of flushing agent (2 tbsp lemon-scented dish detergent per gal water) into each cylinder. Larval sampling occurred on June 5 (28 DAT), July 3 (56 DAT), August 6 (90 DAT), and September 4 (119 DAT) of 2012. Many plots had zero BCW larvae during sampling at 28 DAT and 56 DAT. Thus, data were not normally distributed and could not be analyzed with parametric statistics. Nonparametric Wilcoxon rank sum tests (PROC NPAR1WAY, SAS 9.2) were used to determine global differences among means for each sample date. Pairwise comparisons of treatments were analyzed using Kruskal- Wallis chi-square tests (PROC NPAR1WAY, SAS 9.2). Differences among treatments were highly significant at 28 and 56 DAT (P ≤ 0.0005) (Table 1). With the exception of Meridian 25WG, all treatments were significantly different from the non-treated check and no live BCW larvae were recovered from those plots at 28 DAT. Therefore, 100% control was achieved 4 weeks following treatment with DPX- HGW86 20SC, Acelepryn 1.67SC, and combinations of these two compounds at the rates tested. Additionally, treating with a combination of Meridian 25WG and DPX-HGW86 20SC resulted in 100% control even though Meridian 25WG as a stand-alone treatment did not control BCW. At 56 DAT, only the Acelepryn 1.67SC treatments and the combination of Acelepryn 1.67SC and DPX-HGW86 20SC resulted in 100% control of BCW larvae. These treatments and the combination of Meridian 25WG and DPX-HGW86 20SC were significantly different from the non-treated check. In contrast, both DPX- HGW86 20SC treatments and the Meridian 25WG treatment were not significantly different from the non-treated check. At 90 and 119 DAT, no treatment differences were found (Table 1). Overall, Acelepryn 1.67SC and DPX-HGW86 20SC provided complete control of BCW at 28 DAT when applied at rates of 4 and 8 fl oz per acre or when tank mixed at 4 fl oz per acre each. Combining Meridian 25WG with DPX-HGW86 also provided complete control of BCW, although the latter appears to be the key compound responsible for controlling BCW 1 Arthropod Management Tests 2013, Vol. 38 doi: 10.4182/amt.2013.G1 because Meridian 25WG did not provide any control when applied as a stand-alone treatment. At 56 DAT, only Acelepryn 1.67SC applied at either rate provided complete control of BCW. Although the combination of Acelepryn 1.67SC and DPX-HGW86 20SC also provided complete control, the former appears to be the key compound responsible for controlling BCW because DPX-HGW86 20SC did not provide any control when applied as a stand-alone treatment. However, data from the combination of Meridian 25WG and DPX-HGW86 20SC suggest that higher application rates of the latter compound (i.e., 10.3 fl oz per acre) may be necessary to achieve some control of BCW. None of the treatments provided sufficient control of BCW at 90 DAT and later. Thus, two applications of Acelepryn 1.67SC at rates of 4 or 8 fl oz per acre may be needed under heavy insect pressure to protect bentgrass putting greens from BCW in Oklahoma. Phytotoxicity was not observed for any treatments during the trial. Table 1. Mean (±S.E.) BCW per Cage Treatment/formulation Rate (lb ai/acre) 28 DAT 56 DAT 90 DAT 119 DAT Non-treated check --- 15.5 (1.9)a 12.5 (2.2)ab 7.0 (1.4)a 14.5 (0.7)a Meridian 25WG 0.266 17.3 (1.3)a 7.5 (3.0)bc 8.8 (3.7)a 11.3 (1.9)a DPX-HGW86 20SC 0.052 0 (0)b 18.0 (1.9)a 6.0 (1.3)a 12.0 (2.9)a DPX-HGW86 20SC 0.104 0 (0)b 9.3 (2.9)bc 8.3 (1.9)a 13.5 (3.5)a Meridian 25WG + 0.134 0 (0)b 4.3 (1.3)c 7.8 (3.1)a 13.3 (2.8)a DPX-HGW86 20SC 0.134 Acelepryn 1.67SC 0.052 0 (0)b 0 (0)d 10.8 (2.3)a 15.3 (1.4)a Acelepryn 1.67SC 0.104 0 (0)b 0 (0)d 3.8 (2.4)a 15.3 (5.4)a Acelepryn 1.67SC + 0.052 0 (0)b 0 (0)d 12.8 (2.3)a 18.5 (2.1)a DPX-HGW86 20SC 0.052 Means within columns followed by the same letter are not significantly different (Kruskal-Wallis chi-square, P > 0.05).

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Arthropod Management Tests – Oxford University Press

Published: Jan 1, 2013

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