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Abstract
agriculture Review The Critical Role of Zinc in Plants Facing the Drought Stress 1 1 2 1 Muhammad Umair Hassan , Muhammad Aamer , Muhammad Umer Chattha , Tang Haiying , 3 4 5 6 7 Babar Shahzad , Lorenzo Barbanti , Muhammad Nawaz , Adnan Rasheed , Aniqa Afzal , 1 1 , Ying Liu and Huang Guoqin * Research Centre on Ecological Sciences, Jiangxi Agricultural University, Nanchang 330045, China; muhassanuaf@gmail.com (M.U.H.); muhammadaamer@jxau.edu.cn (M.A.); thy39661026@jxau.edu.cn (T.H.); liuying632084441@163.com (Y.L.) Department of Agronomy, University of Agriculture, Faisalabad 38040, Pakistan; drumer@uaf.edu.pk Tasmanian Institute of Agriculture, University of Tasmania, Hobart 7005, Australia; Babar.shahzad@utas.edu.au Department of Agricultural and Food Sciences, University of Bologna, 40127 Bologna, Italy; lorenzo.barbanti@unibo.it Department of Agricultural Engineering, Khwaja Fareed University of Engineering and Information Technology, Rahim Yar Khan 64200, Pakistan; dmnawaz@kfueit.edu.pk Key Laboratory of Crops Physiology, Ecology and Genetic Breeding, Ministry of Education/College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China; adnanbreeder@yahoo.com Department of Plant Pathology, University of Agriculture, Faisalabad 38040, Pakistan; aniqaafzaluaf@gmail.com * Correspondence: hgqjxauhgq@jxau.edu.cn or hgqjxes@sina.com Received: 30 July 2020; Accepted: 1 September 2020; Published: 7 September 2020 Abstract: Drought stress a ects plant growth and development by altering physiological and biochemical processes resulting in reduced crop productivity. Zinc (Zn) is an essential micronutrient that plays fundamental roles in crop resistance against the drought stress by regulating various physiological and molecular mechanisms. Under drought stress, Zn application improves seed germination, plant water relations, cell membrane stability, osmolyte accumulation, stomatal regulation, water use eciency and photosynthesis, thus resulting in significantly better plant performance. Moreover, Zn interacts with plant hormones, increases the expression of stress proteins and stimulates the antioxidant enzymes for counteracting drought e ects. To better appraise the potential benefits arising from optimum Zn nutrition, in the present review we discuss the role of Zn in plants under drought stress. Our aim is to provide a complete, updated picture in order to orientate future research directions on this topic. Keywords: aquaporins; cell signaling; drought stress; hormone; photosynthesis; ROS; Zn 1. Introduction Abiotic stresses a ect all living organisms present on Earth. Among them, drought stress has devastating impacts on agricultural production [1]. Drought stress is becoming more frequent due to unpredictable rainfalls and changes in climate patterns [2]. The rapid increase in atmospheric temperature has increased crop exposure to drought induced stress [3,4]. Its severity is unpredictable, as it depends upon many factors including the distribution and amount of rainfall, evapotranspiration and soil ability to store moisture [5]. Drought stress diminishes crop productivity by reducing water uptake, leaf water status and gas exchange rates [6]. Moreover, it reduces stomatal conductance, which in turn increases leaf temperature Agriculture 2020, 10, 0396; doi:10.3390/agriculture10090396 www.mdpi.com/journal/agriculture Agriculture 2020, 10, 0396 2 of 20 and leads to wilting [6,7]. The water deficit conditions have further impacts such as disturbing membrane permeability, nutrient uptake and chlorophyll synthesis, thus negatively a ecting plant photosynthetic eciency [8–11]. Although water deficit imposes severe impacts on plants, plants modulate drought-induced e ects by activating the antioxidant defense system that scavenges the reactive oxygen species (ROS), initiating a wide range of physio-chemical responses [12]. The ROS damage plant cell membranes and macro-molecules including proteins and lipids [13]. Agricultural areas around the globe are already facing a rapid reduction in the supply of irrigation water [14], thus it is urgent to adopt policies in order to protect the crops from extreme weather events including drought. The development of drought tolerant crops is one of the imperative approaches to cope with this adversity; however, this approach is time consuming and requires big investments. Conversely, there are other management approaches that can provide quick solutions to this problem. Among them, the use of the mineral nutrients is considered one of the most promising approaches to diminish the e ect of drought. The role of mineral nutrients, specifically Zn, is well understood in plant growth and development under normal conditions. Conversely, Zn role in drought tolerance is still poorly understood. Zinc is an indispensable micronutrient for crop growth, an important component of carbonic anhydrase and a stimulator of aldolase, which are involved in carbon metabolism [15]. Zn is also an integral component of several biomolecules such as lipids, proteins and co-factor of auxins, and, therefore, it plays an important role in plant nucleic acid metabolism [16]. Zn application has been proved beneficial in improving crop yield and quality [17,18], while its deficiency reduces yield and deteriorates crop quality [19]. Moreover, higher Zn contents have toxic e ects on plants and lead to the suppression of cell division and elongation, a ecting biomass production [15]. Fewer studies have shown that adequate supply of Zn can modulate drought tolerance in di erent crops as wheat, sunflower, tomato and red cabbage [20–22]. However, there is lack of information regarding Zn-induced mechanisms conferring drought tolerance in plants. Therefore, this review addresses di erent e ects of drought stress on plants and shows the pending research gaps in plant physiological, biochemical and molecular aspects and Zn cross talk with other molecules in Zn-induced drought stress tolerance. 2. E ects of Drought Stress on Plants Plants are sessile organisms exposed to biotic as well as abiotic stresses during their life cycles. Drought is a most imperative abiotic stress that has deleterious e ects on plant growth and development under several viewpoints [23,24]. Additionally, drought stress has negative e ects on productivity and quality of crops [25,26]. Plant growth and development depend on cell division, elongation and di erentiation, and drought stress a ects all these processes owing to reduction in turgor pressure, enzymatic activities and energy supply for photosynthesis [27,28]. Plant water potential (PWP) and turgor are decreased under water limited conditions, thus plants cannot function properly under these conditions [23,27,28]. The decrease in relative water content (RWC) is the initial consequence of drought stress [29]. The lower RWC reflects in substantial reductions of leaf water potential, which lead to closing of stomata. The increase in stomatal resistance reduces the rate of transpiration and, therefore, leads to increase in leaf temperature because the process of transpiration is the key mechanism that controls leaf temperature [30,31]. The higher leaf temperature denatures various proteins and enzymes and reduces membrane permeability and, resultantly, various facets of plant metabolism are a ected. These changes are the main reasons for the disturbance in photosynthesis, respiration, mineral nutrition and synthesis of proteins and amino acids [31,32]. The reduction in photosynthesis is the main impact determined by drought [33,34]. Many factors are responsible for this reduction, namely reduced leaf expansion, increased leaf temperature, premature leaf senescence and impaired photosynthetic machinery [35,36]. Moreover, stomatal and non-stomatal factors also inhibit photosynthesis in drought stress [31]. The CO limitation due to closing of 2 Agriculture 2020, 10, 0396 3 of 20 stomata particularly under light saturation leads to accretion of reduced photo-synthetic electron transport components that negatively a ect the photosynthetic eciency. The accretion of such components results in production of reactive oxygen species (ROS) that cause oxidative damages in the chloroplast [30,37]. The reduction in chlorophyll content due to water stress also decreases the photosynthetic rate [24,38]. The key enzyme involved in carbon metabolism in the Calvin cycle is ribulose-bisphosphate carboxylase/oxygenase (Rubisco) [29]. The activity and amount of Rubisco decrease dramatically under water deficiency, which negatively a ects photosynthesis [39]. Drought tolerance is a demanding ability, and the amount of energy required to cope with drought stress is enormous [35]. The major consumers of carbon (C) assimilated through photosynthesis under drought stress are plant roots, which use C for their growth and maintenance [29]. Additionally, environmental conditions a ect plant respiration, and changes in C metabolism may occur owing to diminished photosynthesis and active respiration. The growth rates of plants can be precisely determined as the ratio of CO assimilation to respiration [36]. Drought also disrupts mineral nutrition and ion homeostasis in plant cells [40]. The reduction in water supply under drought stress reduces soil nutrient availability as well as uptake by plant roots, resulting in reduced nutrient concentration in plant tissues [40]. The change in nutrient uptake and transport to shoots is the main consequence of drought stress in plants. The hormones play a role of paramount relevance in regulating several plant processes. Abscisic acid (ABA) is the most prominent plant hormone in response to water deficit conditions [35,41]. ABA is primarily synthesized in the vascular system, whence it is transported to the target tissues. The transport occurs in both xylem and phloem, permitting bi-directional transport between roots and shoots. However, under drought stress, pH significantly increases in the apoplast, determining great retention of ABA, which then functions as a root-to-shoot signal resulting in final reduction in transpiration [42]. The ABA induces the accumulation of late embryogenesis abundant (LEA) proteins, which play a significant role in plant adaptation to drought [26]. Moreover, drought rapidly reduces endogenous cytokinin and gibberellin levels. The cytokinins delay leaf senescence, improving plant resilience under drought stress [35]. Plants accumulate various substances including proteins and amino acids in response to drought stress [43]. Water deficiency also alters the amount of proteins [29], whose contents generally decrease under drought stress owing to reduction in their synthesis. The accumulation of osmolytes as proline provides osmotic adaptation that is the main mechanism for plant resistance against drought stress. Water deficiency increases proline content in plants in two di erent ways: (1) by directly increasing the synthesis of proline; and (2) by reducing the activities of enzymes involved in proline degradation [34]. The accumulation of proline and other osmolytes reduces cell water potential, and therefore increases water uptake under drought stress [34]. Additionally, osmolytes protect delicate cell components from oxidative stress, and the biosynthesis of proline in plants plays a vital role in the energy balance between chloroplasts and mitochondria [44]. In conclusion, drought disrupts various physiological, biochemical and molecular processes determining reductions in growth, yield and quality. 3. General Mechanisms of Plant Response to Drought Stress Plants cannot move from their place; therefore, they must tolerate the abiotic stress. Plants have developed di erent mechanisms of resistance against stress conditions. The plants are multicellular organisms whose response against abiotic stresses is very complex [45]. The mechanisms of plant resistance to drought stress rely on three main strategies: drought escape, avoidance and tolerance. The adaptation of plants to drought can be achieved through di erent physiological, morphological, bio-chemical and molecular responses. 4. Zinc Uptake and Transport in Plants Plant species have a broad range of Zn requirement and concentration, owing to variations in Zn uptake from soil, transport from roots to shoots and sequestration in plant organs [46]. Plant Agriculture 2020, 10, 0396 4 of 20 2+ roots take up Zn in divalent form (Zn ), although in few cases plant roots also absorb Zn in the form of organic ligand–Zn complexes. Moreover, plants use two di erent strategies to take up Zn in divalent form depending on the ligands secreted by the roots [46]. The first approach involves the euxes of reductants as organic acids (OA) and H ions that increase the solubility of Zn 2+ complexes (Zn phosphates and hydroxides), resulting in release of Zn ions for the absorption the root epidermal cells. The second strategy involves the eux of phyto-siderophores (lower molecular weight compounds) that make stable complexes with Zn fostering Zn influx into the root epidermal cells. However, this strategy is only found in the roots of cereal crops [46]. The passive uptake of Zn involves the participation of water molecules, and variations in Zn contents across the root cells plasma membranes (RCPM). The major driving energy involved in the 2+ uptake of Zn comes from hyper-polarization of RCPM, which is governed by the activities of RCPM + + H -ATPase system. The activities of this system help in pumping H ions extra-cellularly at the expense of ATP hydrolysis. The release of H ions in root rhizosphere results in hyper-polarization of RCPM and decreases soil pH, which in turn increases the rate of cation uptake. However, the charged 2+ Zn ions cannot cross the cell membrane freely [47,48], thus specific transporter proteins help in the 2+ transport of Zn cations [49,50] (Table 1). Many genes have been recognized that either encode the proteins involved in Zn transport or regulate the expression for optimum Zn transport. The most imperative proteins involved in the transport of Zn are: the ZIP family proteins (Zn, iron-permease family/ZRT-, IRT proteins), the heavy metal ATPase (HMA) family and the metal tolerant proteins (MTP) family. Among these families, the members of the ZIP family are involved in Zn influx into cell cytosol, while HMA are involved in Zn eux into the apoplast. Moreover, the MTP family is involved in Zn sequestration into the vacuoles and endoplasmic reticulum [46]. The transporter proteins have no close linking with the breakdown of ATP, which supports the passive more than the active Zn uptake 2+ mechanism. Moreover, uptake of Zn also occurs with the help of non-selective cation channels linked with passive fluxes of di erent cations groups [51]. The additional driving force involved in the uptake of metal cations can be due to lower cytoplasmic activities, which is a result of metal sequestration and binding to the intra-cellular sites including, Zn finger proteins, and enzymes [46]. Table 1. Zn transporter proteins in di erent plants. Protein Crop/Plant Function Reference Transport of Zn from ear ZmZLP1 Maize [52] to cytoplasm Involved in export of Zn AtHMA1-AtHMA4 A. thaliana [53] out of plastids Involved in metal Zn AhHMA4 A. halleri partitioning amid root [54] and shoot Enhances Zn OsYSL13, Rice translocation from [55] OsYSL14 senescing leaves to seed Involved in Zn AtMTP1 and A. thaliana partitioning [56] AtMTP3 among organs Involved in Zn transport AtPCR2 A. thaliana [57] and xylem loading OsVIT1 and Mediates Zn transport Rice [58] OsV1T2 from flag leaf to grain 2+ After passing the barriers of casparian strips in roots, Zn enters the living cells of the peri-cycle and 2+ xylem parenchyma symplastically. Moreover, another barrier to Zn transport also occurs at this stage known as xylem loading. The continuous activities of H -ATPase in the xylem parenchyma result in 2+ hyper-polarization of membranes that limit the movement of positive ions (Zn ) out of cell cytosol. 2+ Therefore, the Zn loading from the cells of xylem to apo-plastic xylem is an active process [59]. 2+ The active eux of symplastic Zn is governed by particular transporters (HMA family) which Agriculture 2020, 10, 0396 5 of 20 are present on the xylem parenchyma. The increased activities and high expression of membrane H -ATPase in parenchyma of xylem are also accountable for the acidic nature (pH: 5–6.5) of xylem sap. 2+ Moreover, in the xylem sap, the metal ions including Zn are transported as forming metal complexes with OA, nicotian-amine histidine and asparagine. After entering the phloem, Zn is further translocated into diverse plant organs and sinks governed by short as well as long distance pathways. Zn mobility is quite high in phloem compared to xylem, owing to greater concentration of solutes including the OA and peptides in phloem sap [46]. Moreover, Zn is transported in phloem tissues in ionic forms or as metal complexes. Generally, xylem has lower solute contents; however, it is fundamental for nutrient transport to di erent organs [46]. 5. Roles of Zn in Plants under Drought Stress 5.1. E ects of Zn on Plant Growth and Development under Drought Stress Seed germination is the first stage of plant life a ected by the drought stress. In turn, seed germination and seedling emergence influence plant density and final productivity [60]. The variation in seedling emergence due to environmental factors is of paramount importance in view of crop management. The interaction among environmental factors and internal seed mechanisms determine the level of germination and emergence under specific conditions [60]. Zn application (seed priming) improves germination and yield of maize, wheat and chickpea under a wide range of environmental conditions [61,62]. The drought stress reduces plumule length and dry weight, owing to reduced remobilization of nutrients from reserves to embryo. The application of Zn (priming) improves the synthesis of IAA and GA under drought conditions [63], and the synthesis of these hormones augments plumule length and weight under drought stress. Zn is a fundamental nutrient for plants as it plays a vital role as metal component and co-factor of many enzymes [64]. The cell membrane is the first target of abiotic stresses [65], and the maintenance of its stability under drought is the core part of drought tolerance [66]. Adequate Zn supply in drought stress maintains membrane permeability, the activity of antioxidant substances, photosynthetic eciency and WUE [67–69]. Moreover, Zn application results in appreciable increase in leaf area, the content of chlorophyll and other photosynthetic pigments, and stomatal conductance, thus resulting in improved growth and yield [70]. Similarly, Sultana et al. [71] noted that Zn countered the adverse impact of drought and remarkably increased wheat productivity. In another study, Chattha et al. [17] noted that Zn application improved maize yield and harvest index in drought stress. Moreover, Hera et al. [65] noted that foliar applied Zn diminished the negative impacts of water deficit and increased growth and yield of wheat. Therefore, Zn application counteracts drought by improving membrane stability, hormone synthesis, the photo-synthetic process and the scavenging of ROS (Table 2). 5.2. E ect of Zn on Plant Water Relations in Drought Stress Leaf water potential, relative water content (RWC), stomatal conductance and leaf temperature directly a ect the plant water relations. The RWC is a main physiological trait which determines plant drought tolerance ability [72,73]. Zn application improves chlorophyll, RWC and carotenoid contents, while it reduces the electrolyte leakage and water loss in drought condition [74]. The application of Zn enhances antioxidant enzyme activities, stomatal conductance and RWC while restraining electrolyte leakage in water deficit situations [75]. Adequate Zn nutrition improves vegetative growth and drought tolerance in alfalfa by enhancing root growth and RWC [76]. The maintenance of good leaf water status is a result of proper control exerted by the guard cells in diverse conditions, which improves the growth and enhances crop survival under stress conditions [77]. Therefore, from this viewpoint, the improvement in RWC mediated by Zn application results in better crop adaptation to water deficit conditions. Zn application supports the RWC owing to the role of Zn in improving tissue formation, osmolyte accumulation and prevention of leaf tissues from destructive e ects of water deficient conditions [78,79]. In conclusion, Zn application Agriculture 2020, 10, 0396 6 of 20 improves the activities of antioxidants and decreases electrolyte leakage, which in turn increases membrane stability and results in increased RWC under drought stress (Table 2). Table 2. E ect of Zn application on growth and physiological characteristics in drought stress. Crop Drought Stress E ects References Zn application increases plant biomass, stomatal conductance Maize 40% field capacity and quantum yield of [80] photosystem II under drought stress. Drought stress at Zn improves grain yield, RWC Maize pollination and [81] and chlorophyll content. seed filling Zn improves seed and oil yield, Drought stress at seed Sunflower chlorophyll and proline contents [82] filling stage and SOD activities. Zn application improves yield, Drought stress at grain Wheat chlorophyll content and activities [83] filling stage of SOD, POD and CAT. Zn application increases the activities of POD, CAT, quantum Water stress at heading Triticale yield of PS-II, chlorophyll content, [75] and booting stomatal conductance and reduces the electrical conductivity. Zn increases plant height, shoot Irrigation applied at biomass, chlorophyll and Common bean interval of 6, 12 and carotenoid contents, leaf NPK [84] 18 days content and decreases the MDA contents Zn supplementation improves the Tomato With-held irrigation stomatal aperture and [85] chlorophyll content. Zn leads to improvement in PS-II eciency, CO assimilation rate, Chickpea 35% field capacity proline content and activities of [86] SOD, APX, while reduces the MDA contents. Zn application increases chlorophyll and carotenoid Chickpea 35% field capacity [87] contents, seedling vigor and seed yield. Zn improves stomatal Drought stress at conductance, WUE, eciency of Wheat heading and [88] PS-II, chlorophyll content and an-thesis stage reduces energy dissipation 5.3. E ect of Zn on Cell Membrane Stability under Drought Stress The extent of membrane injury can be estimated by the measurement of electrolyte leakage from the cell. The amount of the leakage can be correlated with di erent physiological and bio-chemical processes including the synthesis of antioxidant enzymes, WUE, osmotic potential and stomatal regulation [89,90]. Thus, it is not surprising that low electrolyte leakage can be recommended as criteria to identify stress resistance in di erent crop species and genotypes [91]. Drought induces lipid peroxidation in plants, which results in leakage of essential solutes from the cells and organelles, causing damage to membrane functions and several metabolic processes [92]. Thus, the ability of plants to control the movements of ions in and out of cells can be used as test to check damage to plant tissues. Zn has special properties as it exists in divalent state without redox cycling and, therefore, remains stable in biological mediums [93]. Owing to these properties, Agriculture 2020, 10, 0396 7 of 20 Zn protects membrane lipids from ROS, in turn preventing the leakage of ions from the ion channels [90]. Moreover, drought stress enhances malondialdehyde (MDA) content and ion leakage owing to reduction in the activity of antioxidants [91]. The application of Zn improves the activity of antioxidant enzymes such as superoxide dismutase (SOD) and peroxidase (POD) and reduces the e ects of oxidative stress, the accumulation of MDA and electrolyte leakage. Therefore, Zn protects the cellular membranes from drought stress damages (Table 2). 5.4. Regulation of Osmolyte Accumulation by Zn under Drought Stress The osmotic adjustment is the principal mechanism linked with maintenance of higher turgor potential and water retention in plants, in response to drought [94]. The osmotic adjustment is related to accretion of di erent lower molecular weight substances, such as proline, glycine-betaine, soluble sugars (SS) and soluble proteins (SP). Moreover, as the result of osmolyte accumulation, the activity of organelles and cytoplasm takes place at the normal pace, ensuring sustained growth, photosynthetic eciency and assimilate partitioning [29]. The SS and SP diminish drought induced damages by favoring leaf turgor and stomatal conductance for ecient CO intake and promoting the ability of plant roots to take up more water [95,96]. Besides its role in regulating enzyme activity, Zn also improves protein synthesis and carbohydrate metabolism [97]. The application of Zn improves the expression of Zn finger proteins which in turn stimulate SS and proline accumulation under drought conditions [98–100]. Proline accumulation protects not only cells and the enzymes involved in osmo-regulation, but also plants from the osmotic stress [101]. The application of Zn also increases the leaf water potential under drought conditions [20,21]. Moreover, the accumulation of osmolytes augments root water uptake by regulating the leaf water potential, alleviates the oxidative damage and improves drought resistance [13]. Therefore, accumulation of compatible solutes under drought stress owing to Zn supplementation improves drought tolerance. Moreover, solute accumulation owing to Zn application increases water use eciency (WUE), chlorophyll content and dry matter production [100]. Zn application in drought conditions increases the accumulation of soluble carbohydrates, which in turn protect the plants from the deleterious e ects of drought stress [102,103]. Zinc also improves the synthesis of glycine-betaine (GB) in drought stress conditions, which contributes to the increased tolerance [104]. Amino acids are known for their important role of regulating the osmotic homeostasis under stress conditions [105]. Zn application improves the synthesis of the amino acids which protect the plants from drought stress consequences [106,107]. To summarize, Zn application improves the accumulation of compatible solutes which maintain a higher turgor potential and water retention in plants in response to drought. This contributes significantly to crop performance under stress conditions. However, there are still gaps of knowledge concerning the e ects of Zn application on the accumulation of proline, GB, polyamines and polyphenols under drought stress conditions. Therefore, future research should focus these gaps providing clues to a better understanding of the comprehensive role of Zn in higher plants. 5.5. E ect of Zn on Stomatal Regulation and Water Use Eciency under Drought Stress Water use eciency is significantly reduced under drought stress, owing to reduction in stomatal conductance. The application of Zn improves the stomatal regulation thanks to the maintenance of membrane integrity. Zn deficiency leads to reduction of K in guard cells, and this may be associated with the increase in K eux relative to influx, owing to membrane damage caused by Zn deficiency [108]. Nonetheless, the exact role of Zn in regulating the stomatal opening is still unclear and direly needs deeper investigation. Generally, stomata closing in response to drought improves WUE, due to the fact that a greater reduction in transpiration than in photosynthesis occurs [109]. Zn application in drought conditions improves stomatal conductance and favors accumulation of osmolytes, which in turn improve WUE and drought tolerance [109]. Plants consume less water under Agriculture 2020, 10, 0396 8 of 20 drought conditions, but water that is transpired is not eciently used in case of Zn deficiency [109,110]. Zn deficiency under stress conditions increases leaf temperature owing to closing stomata, and increases the proportion of carbon (C) lost in respiration [111]. Under such circumstance, the application of Zn maintains the stomatal regulation and leaf temperature, thus increasing WUE. In another study, Ahmed et al. [112] noticed that Zn application improved photosynthesis, stomatal conductance and transpiration, and, therefore, it resulted in marked WUE increase under drought stress. In conclusion, Zn application improves the K influx in guard cells, which ameliorates the stomatal conductance resulting in significant WUE increase in drought stress (Table 2). 5.6. E ect of Zn on Nutrient Uptake under Drought Stress The role of mineral nutrients in improving drought tolerance has been studied by many researchers. However, this is still insucient and somewhat intangible. Macronutrients form the structural components of plants, and their deficiency causes symptoms in plants that are readily observed [113]. Conversely, the micronutrients a ect the susceptibility of plants to diverse stress conditions by regulating the enzymatic activities, and modulating signal transductions and accumulation of compatible solutes [114]. There are many reports indicating the noxious impacts of nutrient deficiency on photosynthesis under drought stress. However, the studies related to the e ect of drought on mineral nutrient uptake and their e ect on plant physiology still have many dark areas [29,113]. Limited information is available about the e ects of Zn application on nutrient uptake in drought conditions. In the literature, the exogenously applied Zn markedly increased leaf Zn content [115], as well as the uptake of other nutrients, i.e., iron and manganese [116]. The e ect of Zn on nutrient uptake and its interaction with other nutrients are well documented under normal conditions. However, they are still unclear under drought stress and need to be elucidated. Therefore, future research should better focus the e ects of Zn application on nutrient uptake and its interaction with other nutrients under drought stress (Table 2). 5.7. E ect of Zn on Photosynthesis under Drought Stress The abiotic stresses faced by plants, a ect several physiological and biochemical mechanisms. Zn has many functions in plants as it is a structural catalytic and co-catalytic component of more than 300 enzymes including carbonic anhydrase, carboxy-peptidase, alcohol dehydrogenase, Cu/Zn superoxide dismutase, fructose 1,6 bisphosphatase and aldolase [113]. Zn deficiency reduces the activity of carbonic anhydrase a ecting photosynthesis. The application of Zn repairs the PS-II processes and integrates the Rubisco structure, improving photosynthesis under drought stress [15,117]. Furthermore, Zn supplementation under water deficit conditions improves chlorophyll content and photosynthetic rates, alleviating the e ects of drought [69]. Sub-stomatal CO concentration (Ci) increases under drought stress owing to low photosynthetic rate. The reduction in photosynthesis rate (Pn) associated with an increase in Ci has been frequently interpreted as a direct e ect of drought stress on plant photosynthetic capacity [118]. Zn application leads to reduction in Ci under drought conditions and, therefore, improves Pn. Zn application also improves photosynthesis under drought by its direct e ect on stomata opening, detoxification of ROS and activation of enzymes [103]. Drought reduces quantum yield owing to decline in chlorophyll content. However, Zn application under drought increases chlorophyll content and quantum yield, resulting in significant increase in photosynthesis under drought stress [104]. The application of Zn substantially increases chlorophyll content, Fv/Fm and photosynthetic characteristics under drought stress [70,119,120]. Moreover, Zn application improves chlorophyll synthesis owing to the fact that Zn is a structural component of di erent proteins and enzymes and a co-factor for normal biosynthesis of pigments [121]. In addition, Zn stabilizes membrane permeability and integrity under water stress, which results in increased photosynthesis [120,122]. Moreover, Zn a ects the concentration of nutrients involved in the synthesis of chlorophyll and availability of other nutrients including nitrogen and manganese, which serve as constituents in the molecular structure of Agriculture 2020, 10, 0396 9 of 20 chlorophyll [104,123]. Zn also triggers the enzymes involved in the chlorophyll biosynthetic pathway, and the activities of antioxidant enzymes which improve photosynthesis in drought stress [124]. Therefore, Zn application improves enzymatic activities, chlorophyll content and Pn; detoxifies ROS, and maintains the Rubisco structure. All this leads to significant improvements in the smoothness of the photosynthesis process under drought stress (Table 2). 5.8. Zn-Mediated Cross Talk with Hormones under Drought Stress Plant growth regulators (PGR), when applied exogenously, and phyto-hormones, when produced internally, substantially a ect plant physiological processes. Both terms are used inter-changeably, when referring to auxins, namely indol acetic acid (IAA), gibberellins (GA), cytokinis (CK), ethylene and abscisic acid (ABA) [125]. Phyto-hormones play an appreciable role in drought tolerance [29]. Zn application substantially influences the IAA level, and Zn is known to be co-enzyme for the synthesis of tryptophane that is a precursor to the formation of IAA [117,126]. The application of Zn under drought stress increases the IAA levels, which in turn improve root growth, facilitate water uptake, and therefore improve drought tolerance [117]. Water deficiency significantly reduces IAA and GA contents, and Zn application significantly improves the levels of IAA and GA reflecting in improved plant performance [127]. The application of Zn highly improves the metabolic pathways of tryptophan in drought conditions. Therefore, being the precursor of IAA and melatonin, higher concentration of tryptophan alleviates the e ects of drought by reducing the oxidative damages and regulating the osmotic balance [128]. The initial precursors of melatonin synthesis are tryptophan, which is catalyzed by tryptophan decarboxylase (TDC), and tryptamine 5-hydroxylase (T5H). Together, they synthesize the intermediates of melatonin, i.e., tryptamine and serotonin [129]. Then serotonin is converted into melatonin by serotonin N-acetyltransferase (SNAT) and N-acetylserotonin O-methyltransferase (ASMT)/ca eic acid O-methyltransferase (COMT). Additionally, tryptophan is a precursor for IAA production, which maintains plant growth and osmotic balance by regulating vacuole osmotic pressure under drought [130]. Melatonin and serotonin play a vital role against various abiotic stresses. The higher melatonin content regulates the nitro-oxidative balance, proline metabolism and the antioxidant defense. Hence, it contributes to biomass production and drought tolerance [131,132]. Zn improves melatonin content in drought stress by increasing the upstream substrate contents and the gene expression of enzymes involved in melatonin biosynthesis, which in turn improves the drought tolerance in plants [133]. However, the mechanisms underlying Zn e ects on ABA, GA, CK, brassinosteroids, jasmonates and ethylene contents in plants are still unknown. Therefore, future studies should be conducted to answer these questions. 5.9. Detoxification of Reactive Oxygen Species by Zn under Drought Stress Drought stress leads to over production of ROS resulting in cell membrane damages, MDA accumulation and cell death. Zn application under drought stress reduces MDA content, demonstrating the critical role of Zn in cell membrane protection from ROS in drought stress [100]. Plants have a sophisticated antioxidant defense system containing various enzymes protecting plants from the ROS under drought stress [134]. SOD contains Cu/Zn-SOD, Mn SOD and Fe-SOD, which constitute the first protective systems against O , and converts it into H O and O [135]. Zn plays a chief role in 2 2 2 2 Cu/Zn-SOD for the elimination of ROS adverse e ects [136]. Additionally, Zn binds with cysteine and histidine to restrict ROS accumulation [15]. The application of Zn improves the expression of zinc-finger proteins, increases the expression of these proteins, enhances the activities of the antioxidant enzymes (Figure 1) and decreases the MDA accumulation [100,137]. It was shown that Zn application under drought stress increases the activities of SOD, CAT and APX in cotton and rice, which suggests that application of Zn significantly enhances the activity of the antioxidant system, thus contributing to the alleviation of oxidative stress caused by drought stress [100,138]. Agriculture 2020, 10, x FOR PEER REVIEW 11 of 20 Agricultur many e 2020 que , 10 stio , 0396 ns that need to be answered, as the particular function of each isoform in response to 10 of 20 Zn. Moreover, other mechanisms of AQPs regulation at protein level need to be further elucidated. Figure 1. Mechanisms of drought tolerance induce by Zn. Zinc application improves the antioxidant Figure 1. Mechanisms of drought tolerance induce by Zn. Zinc application improves the antioxidant activities, increases osmolyte accumulation, hormonal cross talk and cell signaling, which in turn activities, increases osmolyte accumulation, hormonal cross talk and cell signaling, which in turn improve membrane stability, physiological processes including water uptake and ROS scavenging. improve membrane stability, physiological processes including water uptake and ROS scavenging. 6. Methods of Zinc Application to Crops The other lower molecular weight antioxidant substances including ascorbate (ASC), glutathione There are many ways in which Zn can be applied to plants: seed priming, seed coating, soil and (GSH) and phenolics can also improve the resistance against drought stress [139]. Zn supplementation foliar application [18,164]. Seed priming with Zn substantially improves the germination and stand increases ASC and GSH contents and protects plants from the oxidative stress caused by establishment [165]. Micronutrient application as seed treatment successfully delivers the nutrients drought [122,140]. Total phenolic contents (TPC) and total flavonoid contents (TFC) represent amounts aimed for growth and yield enhancement [165]. However, seed treatments with higher Zn (2 g Zn of secondary metabolites protecting plant cells from the attack of ROS. The Zn supplementation under −1 kg seed) concentration significant inhibit seed germination and subsequent growth [166]. Thus, in drought stress improves TPC, TFC and ASC in pistachio leaves and vine berries owing to increased view of a suitable scale of Zn application through seed priming, it is necessary to optimize the level antioxidants biosynthesis protecting the cells from ROS [141,142]. of application. Foliar spray of Zn (0.5%) is also considered a quick Zn application method in The ASC-GHS cycles and biosynthesis of phenolics are involved in the non-enzymatic antioxidant −1 conditions of low Zn availability to improve crop productivity [18]. Foliar application (1.5 kg ha ) is system. MDHAR, DHAR, GS and APX are the imperative enzymes involved in the ASC–GSH a very easy and economical method that markedly increased the yield of wheat and maize [167]. The cycle. The APX and MDAR enzymes play a prominent role in drought tolerance, and Zn application foliar application is most effective owing to the fact that Zn is directly applied onto plant leaves [168]. −1 The foliar applied Zn (140 g Zn L ) significantly increased the starch content, grain yield and Zn considerably increases the activity of these enzymes in drought stress [120,143]. content in the maize crop, which is prone to deficiency in this micronutrient [169]. In another study, 5.10. it E w ect as not of Zn ed on that Str fo ess liar Pr ap oteins pliedunder Zn (30 Dr 0 ppm Zn ought Str -ED essTA) significantly improved growth and yield −1 traits of mungbean [170]. Foliar applied Zn (3 mg L ) significantly improved the physiological traits, The synthesis of stress proteins in plants is a pervasive response against various stresses including yield and quality of beans (Phaseolus vulgaris L.) [171]. Soil application is also a common and the drought stress. Most of the stress proteins are water soluble; thus, they provide significant important method to supply the nutrients needed in larger quantity [172]. Micronutrients including −1 contribution to stress tolerance mechanism by the hydration of cellular structures [144]. The increase Zn can be easily supplied by soil application. Soil applied Zn (12 kg ha ) effectively improved the −1 in the expression of Zn finger proteins increases the accumulation of SS and proline and therefore, grain and biomass yield of maize [173]. Similarly, in another investigation, applied Zn (15 kg ha ) resulted in a significant increase of grain yield and Zn content in the wheat crop [174]. The combined improves drought tolerance [98]. −1 soil and foliar application is also useful to improve crop productivity. Soil (50 kg ZnSO4 ha ) + foliar There is paucity of information available about the e ects of Zn on stress proteins. Likewise, (0.5%) application of Zn significantly increased the yield and grain Zn content of wheat [17]. In C H Zn finger protein is an imperative Zn finger protein that considerably enhances the drought 2 2 resistance by improving the accumulation of osmotic substances. For instance, in rice the over expression of ZFP252 significantly improves the drought tolerance [99]. Likewise, the over expression of OsMSR15 and ZFP3 in Arabidopsis counters the damaging e ects of drought by increasing proline accumulation, reducing the electrolyte leakage and increasing the expression of stress-responsive genes [145,146]. C H Zn finger protein also boosts the drought tolerance by enhancing ROS scavenging. 2 2 Agriculture 2020, 10, 0396 11 of 20 ZFP245 Zn finger protein improves the drought resistance in rice by scavenging the ROS owing to increased activities of SOD and POD [147]. C H Zn finger protein also improves the drought tolerance 2 2 by means of ABA and other signaling pathways [148]. Therefore, the increase in the expression of Zn finger proteins counters the e ects of drought by increasing accretion of compatible solutes, scavenging ROS and a ecting the signaling pathways. There is another group of proteins known as heat shock proteins (HSPs), which are generally produced in plants in response to heat stress. However, some HSPs have been found to be induced under diverse stresses including the drought stress [29,149]. Similarly, drought changes the expression of late embryogenesis abundant (LEA) proteins that prevent the denaturation of plant proteins [150]. However, there is no single study available regarding the e ects of Zn application on the expression of HSPs and LEAs under stress. Therefore, future studies should be conducted to quantify the e ects of Zn application on the expression of these proteins under dry conditions. 5.11. E ect of Zn on the Activities of Aquaporins under Drought Stress Aquaporins (AQPs) are channel proteins located in the plasma and intra-cellular membranes. AQPs play a vital role in plant water relations by regulating membrane hydraulic conductivity, osmotic potential, and changes in water permeability [151]. In water limited conditions, the aquaporin gene expression can be regulated to help the plant in maintaining the water balance [152–154]. Plant AQPs can be sub-divided into the plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), NOD26-like intrinsic proteins (NIPs), small basic intrinsic proteins (SIPs) and uncharacterized intrinsic proteins (XIPs) [155,156]. AQPs are responsible for 75–95% of water transit in plants [157,158]. Zn application modulates the AQPs PIP2;1 and PIP2;5: the two AQP isoforms play a vital role in plant water dynamics as a part of a quick Zn response and osmotic stress prevention [159,160]. The over-expression these two AQPs isoforms favors water transport to the inner tissues of leaves, and they also prevent the decrease in leaf water potential and xylem tension [161,162]. As for the root AQPs, Zn application improves the expression of root AQPs including the PIP1 and PIP1;4 in Arabidopsis in response to drought stress [155]. After the application of Zn, PIP1;4 promotes water transport from roots to aerial parts in order to maintain the water status and gas exchange characteristics in Brassica rapa [160]. The application of Zn modifies the gene expression of AQPs present in the membranes of leaves and roots, and these proteins are involved in the shoot-to-root hydraulic communications enabling the water transport [163]. The application of Zn induces quick hydraulic signals from plant leaves to roots, resulting in changes in the hydraulic conductance regulating water movements [160]. Moreover, Zn application increases the abundance of isoforms such as PIP1, which 2+ in turn improve root hydraulic conductance, photosynthesis and Ca root uptake [160]. Thus, all these changes lead to improvement in growth under drought stress. To summarize, Zn application increases the expression of AQPs, which improve the shoot-to-root hydraulic communications thus favoring the water transport under drought stress. However, there are still many questions that need to be answered, as the particular function of each isoform in response to Zn. Moreover, other mechanisms of AQPs regulation at protein level need to be further elucidated. 6. Methods of Zinc Application to Crops There are many ways in which Zn can be applied to plants: seed priming, seed coating, soil and foliar application [18,164]. Seed priming with Zn substantially improves the germination and stand establishment [165]. Micronutrient application as seed treatment successfully delivers the nutrients aimed for growth and yield enhancement [165]. However, seed treatments with higher Zn (2 g Zn kg seed) concentration significant inhibit seed germination and subsequent growth [166]. Thus, in view of a suitable scale of Zn application through seed priming, it is necessary to optimize the level of application. Foliar spray of Zn (0.5%) is also considered a quick Zn application method in conditions of low Zn availability to improve crop productivity [18]. Foliar application (1.5 kg ha ) Agriculture 2020, 10, 0396 12 of 20 is a very easy and economical method that markedly increased the yield of wheat and maize [167]. The foliar application is most e ective owing to the fact that Zn is directly applied onto plant leaves [168]. The foliar applied Zn (140 g Zn L ) significantly increased the starch content, grain yield and Zn content in the maize crop, which is prone to deficiency in this micronutrient [169]. In another study, it was noted that foliar applied Zn (300 ppm Zn-EDTA) significantly improved growth and yield traits of mungbean [170]. Foliar applied Zn (3 mg L ) significantly improved the physiological traits, yield and quality of beans (Phaseolus vulgaris L.) [171]. Soil application is also a common and important method to supply the nutrients needed in larger quantity [172]. Micronutrients including Zn can be easily supplied by soil application. Soil applied Zn (12 kg ha ) e ectively improved the grain and biomass yield of maize [173]. Similarly, in another investigation, applied Zn (15 kg ha ) resulted in a significant increase of grain yield and Zn content in the wheat crop [174]. The combined soil and foliar application is also useful to improve crop productivity. Soil (50 kg ZnSO ha ) + foliar (0.5%) application of Zn significantly increased the yield and grain Zn content of wheat [17]. In accordance with this, Hassan et al. [18] noted that Zn seed priming (0.3 M ZnSO ), soil (50 kg ZnSO ha ) and 4 4 foliar (0.5% ZnSO ) applications markedly increased the growth, yield and grain Zn contents of wheat. 7. Conclusions and Future Prospects Drought is a major abiotic stress considerably limiting crop productivity across the globe. It causes a variety of adverse e ects in plants, including reduction in seed germination, seedling establishment, biomass and grain yield and nutrient and water uptake. Additionally, drought stress disturbs plant physiological and molecular processes, which in turn have negative impacts on overall plant performance. Nutrient management plays a pivotal role to develop plant resistance against drought stress. Wise nutrient management improves plant resilience through diverse mechanisms. Several researchers report the role of Zn in support of plant drought tolerance through di erent mechanisms: Zn alleviates the drought stress by improving the plant water relations, cell membrane stability, osmolytes accumulation, stomatal regulation and water uptake. Additionally, Zn application increases the endogenous hormones (auxins, gibberellins and melatonin) and improves the activities of aquaporins and the antioxidant system, which in turn support the photosynthetic eciency resulting in significant mitigation of drought stress e ects. The information discussed in this review highlights the important roles of Zn in enhancing drought tolerance in plants. However, there are still several questions which need further investigation. For instance, the role of Zn application on nutrient uptake and its interaction with other nutrients under drought stress is poorly understood. Therefore, future studies should address the interactions between Zn application and other nutrients’ uptake. Moreover, it would be useful to unfold the complexity of Zn signaling in response to drought conditions and to discover if Zn application directly or indirectly enhances the levels of endogenous hormones to counter the e ects of drought stress. Meanwhile, genomic and transcriptomic investigations are direly needed to detect the stress responsive proteins and their roles in drought tolerance. The e ects of Zn application on accumulations of osmolytes, especially proline, glycin-betaine, polyamines and polyphenols, are still not understood; therefore, future studies are required to quantify the e ects of Zn on accumulation of these osmolytes under drought stress. Moreover, there is a paucity of information related to plant ability to adjust osmotically that originates from accumulation of osmolytes, as well as the antioxidative defense resulting from non-enzymatic antioxidants. Similarly, future studies should be undertaken to better understand the e ects of Zn on the expression of both heat shock proteins and late embryogenesis abundant proteins under drought conditions. Lastly, more investigations are needed to elucidate the e ects of Zn on expression of plant aquaporins and their role in plant water relations under drought stress. Author Contributions: Conceptualization, M.U.H., M.U.C. and H.G.; resources, H.G.; data curation, Y.L., A.R., A.A.; writing—original draft preparation, M.U.H., M.A., M.U.C. and H.G.; writing—review and editing, L.B., B.S., T.H. and M.N.; supervision, H.G.; and funding acquisition, H.G. All authors have read and agreed to the published version of the manuscript. Agriculture 2020, 10, 0396 13 of 20 Funding: This work was supported by the National Key R&D Program of China (2016YFD0300208); National Natural Science Foundation of China (41661070); and Key disciplines (construction) of ecology in the 13th Five-Year Plan of Jiangxi Agricultural University. Acknowledgments: The National Key R&D Program of China (2016YFD0300208); National Natural Science Foundation of China (41661070) is gratefully acknowledged for providing funds for this work. Conflicts of Interest: All authors declare no conflict of interest or state. References 1. Qados, A.M.S.A. E ect of salt stress on plant growth and metabolism of bean plant Vicia faba. J. Saudi Soc. Agric. Sci. 2011, 10, 7–15. 2. Whitmore, T.C. The case of tropical rain forests. The sustainable development of forests: Aspiration and the reality. Naturzale 2000, 15, 13–15. 3. Fahad, S.; Bajwa, A.A.; Nazir, U.; Anjum, S.A.; Farooq, A.; Zohaib, A.; Sadia, S.; Nasim, W.; Adkins, S.; Saud, S.; et al. 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Agriculture – Multidisciplinary Digital Publishing Institute
Published: Sep 7, 2020