Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Nicotine Quantification In Vitro: A Consistent Dosimetry Marker for e-Cigarette Aerosol and Cigarette Smoke Generation

Nicotine Quantification In Vitro: A Consistent Dosimetry Marker for e-Cigarette Aerosol and... AbstractThe e-cigarette category is evolving rapidly, providing consumers with a variety of formats, ranging from cig-a-like products to larger, high-powered modular devices. When generating an in vitro assessment approach across such diverse products, dosimetry considerations are paramount. In this article, we have compared nicotine quantification techniques in two studies using a Vitrocell VC 10 Smoking Robot to generate aerosols from different e-cigarettes. In Study 1, a 3R4F reference cigarette and four different commercially available e-cigarettes were compared: puff-by-puff nicotine concentration was quantified at the same e-cigarette puffing regime (CRM No81) or with different puff durations, (2 or 3 seconds), comparing 3R4F puff-by-puff yields following ISO and HCI smoking regimes. In Study 2, 3R4F and one e-cigarette were assessed for puff-by-puff nicotine concentration in different locations (China and United Kingdom) comparing different nicotine quantification methods with gas chromatography–mass spectrometry and UPLC-MS/MS used in the two laboratories. Study 1 showed that 3R4F cigarette delivers different nicotine concentrations across the different regimes and puff number, supporting the nicotine methodology; e-cigarettes tested generated different amounts of nicotine across the devices tested, but showed consistent puff-by-puff delivery per device. Study 2 showed positive agreement between results across two different laboratories utilizing different methods for nicotine quantification; statistical analysis, combining all interlaboratory variables, indicated that laboratory differences and the interaction of laboratory and puff number were not significant (p = 0.067 and 0.960, respectively). These studies will add further knowledge to support the in vitro assessment of novel nicotine products, providing reliability and assurance in the area of in vitro dosimetry. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied In Vitro Toxicology Mary Ann Liebert

Nicotine Quantification In Vitro: A Consistent Dosimetry Marker for e-Cigarette Aerosol and Cigarette Smoke Generation

Nicotine Quantification In Vitro: A Consistent Dosimetry Marker for e-Cigarette Aerosol and Cigarette Smoke Generation

Applied In Vitro Toxicology , Volume 3 (1): 14 – Mar 1, 2017

Abstract

AbstractThe e-cigarette category is evolving rapidly, providing consumers with a variety of formats, ranging from cig-a-like products to larger, high-powered modular devices. When generating an in vitro assessment approach across such diverse products, dosimetry considerations are paramount. In this article, we have compared nicotine quantification techniques in two studies using a Vitrocell VC 10 Smoking Robot to generate aerosols from different e-cigarettes. In Study 1, a 3R4F reference cigarette and four different commercially available e-cigarettes were compared: puff-by-puff nicotine concentration was quantified at the same e-cigarette puffing regime (CRM No81) or with different puff durations, (2 or 3 seconds), comparing 3R4F puff-by-puff yields following ISO and HCI smoking regimes. In Study 2, 3R4F and one e-cigarette were assessed for puff-by-puff nicotine concentration in different locations (China and United Kingdom) comparing different nicotine quantification methods with gas chromatography–mass spectrometry and UPLC-MS/MS used in the two laboratories. Study 1 showed that 3R4F cigarette delivers different nicotine concentrations across the different regimes and puff number, supporting the nicotine methodology; e-cigarettes tested generated different amounts of nicotine across the devices tested, but showed consistent puff-by-puff delivery per device. Study 2 showed positive agreement between results across two different laboratories utilizing different methods for nicotine quantification; statistical analysis, combining all interlaboratory variables, indicated that laboratory differences and the interaction of laboratory and puff number were not significant (p = 0.067 and 0.960, respectively). These studies will add further knowledge to support the in vitro assessment of novel nicotine products, providing reliability and assurance in the area of in vitro dosimetry.

Loading next page...
 
/lp/mary-ann-liebert/nicotine-quantification-in-vitro-a-consistent-dosimetry-marker-for-e-zJmGDGm0oS
Publisher
Mary Ann Liebert
Copyright
© Jason Adamson et al., 2017; Published by Mary Ann Liebert, Inc.
ISSN
2332-1512
eISSN
2332-1539
DOI
10.1089/aivt.2016.0025
Publisher site
See Article on Publisher Site

Abstract

AbstractThe e-cigarette category is evolving rapidly, providing consumers with a variety of formats, ranging from cig-a-like products to larger, high-powered modular devices. When generating an in vitro assessment approach across such diverse products, dosimetry considerations are paramount. In this article, we have compared nicotine quantification techniques in two studies using a Vitrocell VC 10 Smoking Robot to generate aerosols from different e-cigarettes. In Study 1, a 3R4F reference cigarette and four different commercially available e-cigarettes were compared: puff-by-puff nicotine concentration was quantified at the same e-cigarette puffing regime (CRM No81) or with different puff durations, (2 or 3 seconds), comparing 3R4F puff-by-puff yields following ISO and HCI smoking regimes. In Study 2, 3R4F and one e-cigarette were assessed for puff-by-puff nicotine concentration in different locations (China and United Kingdom) comparing different nicotine quantification methods with gas chromatography–mass spectrometry and UPLC-MS/MS used in the two laboratories. Study 1 showed that 3R4F cigarette delivers different nicotine concentrations across the different regimes and puff number, supporting the nicotine methodology; e-cigarettes tested generated different amounts of nicotine across the devices tested, but showed consistent puff-by-puff delivery per device. Study 2 showed positive agreement between results across two different laboratories utilizing different methods for nicotine quantification; statistical analysis, combining all interlaboratory variables, indicated that laboratory differences and the interaction of laboratory and puff number were not significant (p = 0.067 and 0.960, respectively). These studies will add further knowledge to support the in vitro assessment of novel nicotine products, providing reliability and assurance in the area of in vitro dosimetry.

Journal

Applied In Vitro ToxicologyMary Ann Liebert

Published: Mar 1, 2017

There are no references for this article.