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On the Keto Acid Content of Tears

On the Keto Acid Content of Tears Ophthalmologica 164: 71-77 (1972) I. L. Kahan and Z. E rdei In the last years, enzyme activities of many proteins in biolog­ ical fluids became evident. Tear, a biological fluid poor in pro­ teins contains some newly-discovered enzymes [Liotet, 19671 and the enzyme lysozyme known for long [Fleming, 1922]. Enzyme activity of the latter is highly influenced by certain substances similar to depolymerisation end-products of its substrate, the wall- mucopeptide of Micrococcus lysodeicticus. Inhibitory effect of glucosamine | Butler and Rupley, 1967; Chipman and Schimmel, 1968; Chipman and Sharon, 1969; Lehrer and Fcisman, 1967; Neu- berger and Wilson, 1967; Raftery et al., 1968; Shinitzky et al., 1966] and enhancing effect of other substances e.g. (I,/-serine and neuraminic acid were described [Kalian et al., 1966; Juhdsz and Kalian, 1970], Effect on lysozyme activity of pyruvic acid, a substance of important role in many biochemical processes, con­ centration of tear pyruvic acid and origin of the latter from serine by enzymatic deamination of serine, is the topic of the present investigations. Materials and Methods Tears were collected from the conjunctival fornices with the aid of glass capillaries from If» healthy volunteers or from patients with normal conjunctiva (group 1) and from http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Ophthalmologica Karger

On the Keto Acid Content of Tears

Kahán, I.L.; Erdei, Z.
Ophthalmologica , Volume 164 (1): 7 – Jan 1, 2010
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Publisher
Karger
Copyright
© 1972 S. Karger AG, Basel
ISSN
0030-3755
eISSN
1423-0267
DOI
10.1159/000306706
Publisher site
See Article on Publisher Site

Abstract

Ophthalmologica 164: 71-77 (1972) I. L. Kahan and Z. E rdei In the last years, enzyme activities of many proteins in biolog­ ical fluids became evident. Tear, a biological fluid poor in pro­ teins contains some newly-discovered enzymes [Liotet, 19671 and the enzyme lysozyme known for long [Fleming, 1922]. Enzyme activity of the latter is highly influenced by certain substances similar to depolymerisation end-products of its substrate, the wall- mucopeptide of Micrococcus lysodeicticus. Inhibitory effect of glucosamine | Butler and Rupley, 1967; Chipman and Schimmel, 1968; Chipman and Sharon, 1969; Lehrer and Fcisman, 1967; Neu- berger and Wilson, 1967; Raftery et al., 1968; Shinitzky et al., 1966] and enhancing effect of other substances e.g. (I,/-serine and neuraminic acid were described [Kalian et al., 1966; Juhdsz and Kalian, 1970], Effect on lysozyme activity of pyruvic acid, a substance of important role in many biochemical processes, con­ centration of tear pyruvic acid and origin of the latter from serine by enzymatic deamination of serine, is the topic of the present investigations. Materials and Methods Tears were collected from the conjunctival fornices with the aid of glass capillaries from If» healthy volunteers or from patients with normal conjunctiva (group 1) and from

Journal

Ophthalmologica – Karger

Published: Jan 1, 2010

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