Access the full text.
Sign up today, get DeepDyve free for 14 days.
B. Mcrae, R. Semnani, M. Hayes, G. Seventer (1998)
Type I IFNs inhibit human dendritic cell IL-12 production and Th1 cell development.Journal of immunology, 160 9
H. Miller, B. Miller, T. Ishibashi, S. Ryan (1990)
Pathogenesis of laser-induced choroidal subretinal neovascularization.Investigative ophthalmology & visual science, 31 5
G. Jonak, E. Knight (1984)
Selective reduction of c-myc mRNA in Daudi cells by human beta interferon.Proceedings of the National Academy of Sciences of the United States of America, 81 6
T. Sakamoto, Hitomi Sakamoto, S. Sheu, K. Gabrielian, S. Ryan, D. Hinton (1994)
Intercellular gap formation induced by thrombin in confluent cultured bovine retinal pigment epithelial cells.Investigative ophthalmology & visual science, 35 2
K. Fukuzawa, T. Horikoshi (1992)
Inhibitory effect of human fibroblast interferon (HuIFN‐β) on the growth and invasive potential of cultured human melanoma cells in vitroBritish Journal of Dermatology, 126
(1994)
Protein kinase C: History and perspectives; in Kuo JF (ed): Protein Kinase C
H. Kishi, H. Mishima, U. Yamashita (2000)
INVOLVEMENT OF THE PROTEIN KINASE PATHWAY IN MELANIN SYNTHESIS BY CHICK RETINAL PIGMENT EPITHELIAL CELLSCell Biology International, 24
H. Kishi, H. Mishima, Uki Yamashita (1994)
Growth regulation of retinal pigment epithelial (RPE) cells in vitro.Current eye research, 13 9
T. Sakamoto, H. Sanui, T. Ishibashi, T. Kohno, K. Takahira, H. Inomata, L. Kohen, S. Ryan (1994)
Subretinal neovascularization in the rat induced by IRBP synthetic peptides.Experimental eye research, 58 2
Y. Nishizuka (1988)
The molecular heterogeneity of protein kinase C and its implications for cellular regulationNature, 334
S. Kuriyama, T. Ohuchi, Nagahisa Yoshimura, Yoshihito Honda (1991)
Growth factor-induced cytosolic calcium ion transients in cultured human retinal pigment epithelial cells.Investigative ophthalmology & visual science, 32 11
R. Gopalakrishna, Zhen Chen, U. Gundimeda (1993)
Nitric oxide and nitric oxide-generating agents induce a reversible inactivation of protein kinase C activity and phorbol ester binding.The Journal of biological chemistry, 268 36
Taiji Sakamoto, Hitomi Sakamoto, T. Murphy, C. Spee, D. Soriano, Tatsuro Ishibashi, D. Hinton, S. Ryan (1995)
Vessel formation by choroidal endothelial cells in vitro is modulated by retinal pigment epithelial cells.Archives of ophthalmology, 113 4
F. Falciani, P. Ghezzi, M. Terao, G. Cazzaniga, E. Garattini (1992)
Interferons induce xanthine dehydrogenase gene expression in L929 cells.The Biochemical journal, 285 ( Pt 3)
M. Clemens, I. Trayner, J. Menaya (1992)
The role of protein kinase C isoenzymes in the regulation of cell proliferation and differentiation.Journal of cell science, 103 ( Pt 4)
H. Kishi, H. Mishima, U. Yamashita (1994)
Effect of cytokines and prostaglandins on the growth of chick retinal pigment epithelial cells.Current eye research, 13 11
T. Murphy, Taiji Sakamoto, D. Hinton, C. Spee, U. Gundimeda, D. Soriano, R. Gopalakrishna, S. Ryan (1995)
Migration of retinal pigment epithelium cells in vitro is regulated by protein kinase C.Experimental eye research, 60 6
D. Leppert, E. Waubant, M. Bürk, J. Oksenberg, S. Hauser (1996)
Interferon beta‐1b inhibits gelatinase secretion and in vitro migration of human T cells: A possible mechanism for treatment efficacy in multiple sclerosisAnnals of Neurology, 40
T. Sakamoto, T. Ishibashi, H. Kimura, H. Yoshikawa, C. Spee, M. Harris, D. Hinton, S. Ryan (1995)
Effect of tecogalan sodium on angiogenesis in vitro by choroidal endothelial cells.Investigative ophthalmology & visual science, 36 6
Jianhua Xu, S. Rockow, Stevens Kim, W. Xiong, Wei Li (1994)
Interferons block protein kinase C-dependent but not-independent activation of Raf-1 and mitogen-activated protein kinases and mitogenesis in NIH 3T3 cellsMolecular and Cellular Biology, 14
Background: The aim of this study is to see the effect of interferon β (IFN-β) on cell proliferation and the protein kinase C (PKC) signaling pathway. Methods: Proliferation of cultured human retinal pigment epithelium (RPE) cells, with various concentrations of IFN-β, and with or without 3% fetal calf serum (FCS), was assessed by cell counting. Effects of short (3 h) or prolonged (48 h) exposure of RPE cells to natural human IFN-β were assessed by <sup>3</sup>H-thymidine uptake. Cytosolic and membranous PKC activity over time in cells treated with IFN-β and calphostin C was also measured.Results: IFN-β inhibited the increased proliferation by FCS in the prolonged-exposure assay. The PKC inhibitor calphostin C also showed an inhibitory effect on RPE cell growth and <sup>3</sup>H-thymidine uptake in the chronic exposure with FCS. Short treatment with IFN-β had no inhibitory or stimulatory effect on <sup>3</sup>H-thymidine uptake. Cytosolic and membranous PKC activity was strongly upregulated after short IFN-β exposure but returned to original levels after 1 h. PKC activity was downregulated both in the cytosol and membrane after 24 or 48 h. Conclusion: IFN-β inhibited RPE proliferation in vitro and the effect is mediated by upregulation of the PKC pathway.
Ophthalmologica – Karger
Published: Dec 1, 2001
Keywords: Retinal pigment epithelium (RPE); Interferon β (IFN-β); Subretinal neovascularization (SRN); Protein kinase C (PKC); Calphostin C
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.