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Strawberry extract attenuates oxidative stress in 3T3-L1 cells

Strawberry extract attenuates oxidative stress in 3T3-L1 cells BACKGROUND:High levels of reactive oxygen species (ROS) within the adipose tissue promote a disturbed redox balance and influence its function, impairing adipogenesis, inducing insulin resistance and stimulating adipocyte hypertrophy. Supplementation with antioxidant rich foods can reverse some of these effects. Strawberry is well known as a good source of phytochemicals; however, whether strawberry suppresses increased oxidative stress in 3T3-L1 cells remain unclear.OBJECTIVE:The purpose of the present work was to determine the antioxidant potential of a strawberry extract in 3T3-L1 mouse embryo fibroblast cell line.METHODS:3T3-L1 pre-adipocytes were induced to differentiate into adipocytes in the presence or absence of different concentrations of the strawberry extract. At the end of the differentiation period, intracellular ROS production, thiobarbituric acid reactive substances (TBARS) content, as well as superoxide dismutase (SOD) and catalase (CAT) activities and gene expressions were evaluated.RESULTS:In this study, we confirmed that strawberry extract markedly inhibited increased-oxidative stress in 3T3L1 cells by suppressing intracellular ROS production and decreasing TBARS content. Likewise, SOD and CAT activities and gene expressions were increased.CONCLUSIONS:This paper provides evidence that strawberry extract is able to scavenging free radicals and activate endogenous defense systems, highlighting its potential capacity to modulate obesity induced- inflammatory states. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Berry Research IOS Press

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References (31)

Publisher
IOS Press
Copyright
Copyright © 2018 © 2018 – IOS Press and the authors. All rights reserved
ISSN
1878-5093
DOI
10.3233/JBR-180313
Publisher site
See Article on Publisher Site

Abstract

BACKGROUND:High levels of reactive oxygen species (ROS) within the adipose tissue promote a disturbed redox balance and influence its function, impairing adipogenesis, inducing insulin resistance and stimulating adipocyte hypertrophy. Supplementation with antioxidant rich foods can reverse some of these effects. Strawberry is well known as a good source of phytochemicals; however, whether strawberry suppresses increased oxidative stress in 3T3-L1 cells remain unclear.OBJECTIVE:The purpose of the present work was to determine the antioxidant potential of a strawberry extract in 3T3-L1 mouse embryo fibroblast cell line.METHODS:3T3-L1 pre-adipocytes were induced to differentiate into adipocytes in the presence or absence of different concentrations of the strawberry extract. At the end of the differentiation period, intracellular ROS production, thiobarbituric acid reactive substances (TBARS) content, as well as superoxide dismutase (SOD) and catalase (CAT) activities and gene expressions were evaluated.RESULTS:In this study, we confirmed that strawberry extract markedly inhibited increased-oxidative stress in 3T3L1 cells by suppressing intracellular ROS production and decreasing TBARS content. Likewise, SOD and CAT activities and gene expressions were increased.CONCLUSIONS:This paper provides evidence that strawberry extract is able to scavenging free radicals and activate endogenous defense systems, highlighting its potential capacity to modulate obesity induced- inflammatory states.

Journal

Journal of Berry ResearchIOS Press

Published: Jan 1, 2018

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