Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Session 2: Autoimmunity

Session 2: Autoimmunity Human Antibodies 11 (2002) 9–12 IOS Press Monday 16 September 2002, Moderators: Y. Shoenfeld and K. Potter [14.15–14.35] Production of human monoclonal antibodies to desmoglein 3 from Pemphigus vulgaris patient Kailash C. Bhol and A. Razzaque Ahmed Harvard School of Dental Medicine, Boston, USA Pemphigus vulgaris is a potentially fatal autoimmune mucocutaneous disease associated with production of IgG autoantibodies to desmoglein 3, a 130 kDa epidermal protein. To further characterize the epitope(s) of Pemphigus vulgaris antigen we established two humanhuman hybridomas by fusion of the peripheral blood mononuclear cells with a human and mouse heterohybridoma. These hybridomas designated as Mab Dsg3:06 and Mab Dsg-3:10 are stable in culture and demonstrated yields of monoclonal antibodies specific for Pemphigus vulgaris. Immunofluorescence, immunoblot, ELISA assays demonstrated that both the monoclonal antibodies bind to the intercellular cement substance and to 130 kDa protein present in the skin and specifically binds to recombinant desmoglein 3 protein, but not to desmoglein1 protein. The epitope mapping experiment using 12 peptides spanning the extracellular domain of Pemphigus vulgaris antigen demonstrated that both the antibodies recognized the Bos 6 peptide and are of IgG1 subclass in nature. Both the antibodies were non-pathogenic as demonstrated in vitro by http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Human Antibodies IOS Press

Session 2: Autoimmunity

Human Antibodies , Volume 11 (1) – Jan 1, 2002

Loading next page...
 
/lp/ios-press/session-2-autoimmunity-4fH4LfZflC

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
IOS Press
Copyright
Copyright © 2002 by IOS Press, Inc
ISSN
1093-2607
eISSN
1875-869X
Publisher site
See Article on Publisher Site

Abstract

Human Antibodies 11 (2002) 9–12 IOS Press Monday 16 September 2002, Moderators: Y. Shoenfeld and K. Potter [14.15–14.35] Production of human monoclonal antibodies to desmoglein 3 from Pemphigus vulgaris patient Kailash C. Bhol and A. Razzaque Ahmed Harvard School of Dental Medicine, Boston, USA Pemphigus vulgaris is a potentially fatal autoimmune mucocutaneous disease associated with production of IgG autoantibodies to desmoglein 3, a 130 kDa epidermal protein. To further characterize the epitope(s) of Pemphigus vulgaris antigen we established two humanhuman hybridomas by fusion of the peripheral blood mononuclear cells with a human and mouse heterohybridoma. These hybridomas designated as Mab Dsg3:06 and Mab Dsg-3:10 are stable in culture and demonstrated yields of monoclonal antibodies specific for Pemphigus vulgaris. Immunofluorescence, immunoblot, ELISA assays demonstrated that both the monoclonal antibodies bind to the intercellular cement substance and to 130 kDa protein present in the skin and specifically binds to recombinant desmoglein 3 protein, but not to desmoglein1 protein. The epitope mapping experiment using 12 peptides spanning the extracellular domain of Pemphigus vulgaris antigen demonstrated that both the antibodies recognized the Bos 6 peptide and are of IgG1 subclass in nature. Both the antibodies were non-pathogenic as demonstrated in vitro by

Journal

Human AntibodiesIOS Press

Published: Jan 1, 2002

There are no references for this article.