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Human Antibodies 11 (2002) 9â12 IOS Press Monday 16 September 2002, Moderators: Y. Shoenfeld and K. Potter [14.15â14.35] Production of human monoclonal antibodies to desmoglein 3 from Pemphigus vulgaris patient Kailash C. Bhol and A. Razzaque Ahmed Harvard School of Dental Medicine, Boston, USA Pemphigus vulgaris is a potentially fatal autoimmune mucocutaneous disease associated with production of IgG autoantibodies to desmoglein 3, a 130 kDa epidermal protein. To further characterize the epitope(s) of Pemphigus vulgaris antigen we established two humanhuman hybridomas by fusion of the peripheral blood mononuclear cells with a human and mouse heterohybridoma. These hybridomas designated as Mab Dsg3:06 and Mab Dsg-3:10 are stable in culture and demonstrated yields of monoclonal antibodies speciï¬c for Pemphigus vulgaris. Immunoï¬uorescence, immunoblot, ELISA assays demonstrated that both the monoclonal antibodies bind to the intercellular cement substance and to 130 kDa protein present in the skin and speciï¬cally binds to recombinant desmoglein 3 protein, but not to desmoglein1 protein. The epitope mapping experiment using 12 peptides spanning the extracellular domain of Pemphigus vulgaris antigen demonstrated that both the antibodies recognized the Bos 6 peptide and are of IgG1 subclass in nature. Both the antibodies were non-pathogenic as demonstrated in vitro by
Human Antibodies – IOS Press
Published: Jan 1, 2002
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